Gelkop S, et al. (2012) Development of unique antibodies directed against each of the six different phosphotyrosine residues within the T cell receptor CD3ζ chain. J Immunol Methods 375, 129-37 22020291

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

Download

Y72-p - CD3Z (mouse) ▲

Modsite: LQDPNQLyNELNLGR SwissProt Entrez-Gene Orthologous residues CD3Z (human): Y72‑p, CD3Z iso3 (human): Y72‑p, CD3Z (mouse): Y72‑p, CD3Z iso2 (mouse): Y72‑p, CD3Z (rat): Y72‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE Lck (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Lck (mouse) transfection of constitutively active enzyme

Y83-p - CD3Z (mouse) ▲

Modsite: NLGRREEyDVLEKKR SwissProt Entrez-Gene Orthologous residues CD3Z (human): Y83‑p, CD3Z iso3 (human): Y83‑p, CD3Z (mouse): Y83‑p, CD3Z iso2 (mouse): Y83‑p, CD3Z (rat): Y83‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE Lck (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Lck (mouse) transfection of constitutively active enzyme

Y111-p - CD3Z (mouse) ▲

Modsite: RNPQEGVyNALQKDK SwissProt Entrez-Gene Orthologous residues CD3Z (human): Y111‑p, CD3Z iso3 (human): Y110‑p, CD3Z (mouse): Y111‑p, CD3Z iso2 (mouse): Y111‑p, CD3Z (rat): Y111‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE Lck (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Lck (mouse) transfection of constitutively active enzyme

Y123-p - CD3Z (mouse) ▲

Modsite: KDKMAEAysEIGTKG SwissProt Entrez-Gene Orthologous residues CD3Z (human): Y123‑p, CD3Z iso3 (human): Y122‑p, CD3Z (mouse): Y123‑p, CD3Z iso2 (mouse): Y123‑p, CD3Z (rat): Y123‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE Lck (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Lck (mouse) transfection of constitutively active enzyme

Y142-p - CD3Z (mouse) ▲

Modsite: GKGHDGLyQGLsTAT SwissProt Entrez-Gene Orthologous residues CD3Z (human): Y142‑p, CD3Z iso3 (human): Y141‑p, CD3Z (mouse): Y142‑p, CD3Z iso2 (mouse): Y142‑p, CD3Z (rat): Y142‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE Lck (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Lck (mouse) transfection of constitutively active enzyme

Y153-p - CD3Z (mouse) ▲

Modsite: sTATKDtyDALHMQT SwissProt Entrez-Gene Orthologous residues CD3Z (human): Y153‑p, CD3Z iso3 (human): Y152‑p, CD3Z (mouse): Y153‑p, CD3Z iso2 (mouse): ‑, CD3Z (rat): Y153‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE Lck (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE Lck (mouse) transfection of constitutively active enzyme