Curated Information
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Home > Curated Information Page > PubMed Id: 20534585
Darwech I, Otero JE, Alhawagri MA, Abu-Amer Y (2010) Tyrosine phosphorylation is required for IkappaB kinase-beta (IKKbeta) activation and function in osteoclastogenesis. J Biol Chem 285, 25522-30 20534585
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S177-p - IKKB (mouse)
Modsite: AKELDQGsLCTsFVG SwissProt Entrez-Gene
Orthologous residues
IKKB (human): S177‑p, IKKB (mouse): S177‑p, IKKB (rat): S177‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  macrophage, monocyte, osteoclast
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
RANKL increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Effect of modification (process):  cell differentiation, induced
Comments:  osteoclast differentiation

S181-p - IKKB (mouse)
Modsite: DQGsLCTsFVGTLQy SwissProt Entrez-Gene
Orthologous residues
IKKB (human): S181‑p, IKKB (mouse): S181‑p, IKKB (rat): S181‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  macrophage, monocyte, osteoclast
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
RANKL increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Effect of modification (process):  cell differentiation, induced
Comments:  osteoclast differentiation

Y188-p - IKKB (mouse)
Modsite: sFVGTLQyLAPELLE SwissProt Entrez-Gene
Orthologous residues
IKKB (human): Y188‑p, IKKB (mouse): Y188‑p, IKKB (rat): Y188‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  macrophage, monocyte, osteoclast
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
RANKL increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Effect of modification (process):  cell differentiation, induced
Comments:  osteoclast differentiation

Y199-p - IKKB (mouse)
Modsite: ELLEQQKyTVTVDYW SwissProt Entrez-Gene
Orthologous residues
IKKB (human): Y199‑p, IKKB (mouse): Y199‑p, IKKB (rat): Y199‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  macrophage, monocyte, osteoclast
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
RANKL increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Effect of modification (process):  cell differentiation, induced
Comments:  osteoclast differentiation