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Home > Curated Information Page > PubMed Id: 20484410
Blancquaert S, et al. (2010) cAMP-dependent activation of mammalian target of rapamycin (mTOR) in thyroid cells. Implication in mitogenesis and activation of CDK4. Mol Endocrinol 24, 1453-68 20484410
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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T172-p - CDK4 (human)
Modsite: YSYQMALtPVVVTLW SwissProt Entrez-Gene
Orthologous residues
CDK4 (human): T172‑p, CDK4 (mouse): T172‑p, CDK4 (rat): T172‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  PC CL 3 (thyroid cell)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  dog, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TSH increase
rapamycin TSH inhibit treatment-induced increase
insulin increase
rapamycin insulin inhibit treatment-induced increase

T246-p - PRAS40 (human)
Modsite: LPRPRLNtsDFQkLK SwissProt Entrez-Gene
Orthologous residues
PRAS40 (human): T246‑p, PRAS40 iso2 (human): T116‑p, PRAS40 iso3 (human): T266‑p, PRAS40 (mouse): T247‑p, PRAS40 (rat): T247‑p, PRAS40 (cow): T246‑p

T252-p - p70S6K (rat)
Modsite: HDGTVTHtFCGTIEY SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T252‑p, p70S6K iso2 (human): T229‑p, p70S6K (mouse): T252‑p, p70S6K (rat): T252‑p, p70S6K iso2 (rat): T229‑p, p70S6K (fruit fly): T238‑p
Characterization
Methods used to characterize site in vivo 2D analysis, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  PC CL 3 (thyroid cell)
Cellular systems studied:  cell lines
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
TSH increase
rapamycin insulin, TSH inhibit treatment-induced increase

T412-p - p70S6K (rat)
Modsite: NQVFLGFtYVAPSVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
Methods used to characterize site in vivo 2D analysis, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  PC CL 3 (thyroid cell)
Cellular systems studied:  cell lines
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TSH increase
NKH_477 increase
insulin increase
wortmannin insulin, NKH_477 inhibit treatment-induced increase
TSH, insulin increase
rapamycin insulin, TSH inhibit treatment-induced increase
PD98059 no change compared to control
amino_acid_starvation insulin, NKH_477 inhibit treatment-induced increase
amino_acids increase
amino_acids insulin, NKH_477 augment treatment-induced increase
acadesine increase
compound_C insulin augment treatment-induced increase
compound_C NKH_477 no effect upon treatment-induced increase
lithium insulin augment treatment-induced increase
lithium NKH_477 no effect upon treatment-induced increase

T444-p - p70S6K (rat)
Modsite: RFIGsPRtPVsPVKF SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T444‑p, p70S6K iso2 (human): T421‑p, p70S6K (mouse): T444‑p, p70S6K (rat): T444‑p, p70S6K iso2 (rat): T421‑p, p70S6K (fruit fly): S429‑p
Characterization
Methods used to characterize site in vivo 2D analysis, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  PC CL 3 (thyroid cell)
Cellular systems studied:  cell lines
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
TSH increase
rapamycin insulin, TSH inhibit treatment-induced increase

S447-p - p70S6K (rat)
Modsite: GsPRtPVsPVKFsPG SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): S447‑p, p70S6K iso2 (human): S424‑p, p70S6K (mouse): S447‑p, p70S6K (rat): S447‑p, p70S6K iso2 (rat): S424‑p, p70S6K (fruit fly): S430‑p
Characterization
Methods used to characterize site in vivo 2D analysis, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  PC CL 3 (thyroid cell)
Cellular systems studied:  cell lines
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
TSH increase
rapamycin insulin, TSH inhibit treatment-induced increase

T247-p - PRAS40 (rat)
Modsite: LPRPRLNtSDFQKLK SwissProt Entrez-Gene
Orthologous residues
PRAS40 (human): T246‑p, PRAS40 iso2 (human): T116‑p, PRAS40 iso3 (human): T266‑p, PRAS40 (mouse): T247‑p, PRAS40 (rat): T247‑p, PRAS40 (cow): T246‑p
Characterization
Methods used to characterize site in vivo [32P] ATP in vitro, phospho-antibody, phosphopeptide mapping, western blotting
Relevant cell lines - cell types - tissues:  PC CL 3 (thyroid cell)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  dog, rat
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (rat)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
NKH_477 increase
insulin increase
6-MB-cAMP insulin augment treatment-induced increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
TORC1 (rat) Disrupts co-immunoprecipitation

S772-p - Rb (rat)
Modsite: STRPPTLsPIPHIPR SwissProt Entrez-Gene
Orthologous residues
Rb (human): S780‑p, Rb (mouse): S773‑p, Rb (rat): S772‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  PC CL 3 (thyroid cell)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  dog, rat

T818-p - Rb (rat)
Modsite: LPtPtKMtPRSRILV SwissProt Entrez-Gene
Orthologous residues
Rb (human): T826‑p, Rb (mouse): T819‑p, Rb (rat): T818‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  PC CL 3 (thyroid cell)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  dog, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
rapamycin insulin, TSH inhibit treatment-induced increase
insulin increase
TSH increase

S235-p - S6 (rat)
Modsite: IAKRRRLssLRAsts SwissProt Entrez-Gene
Orthologous residues
S6 (human): S235‑p, S6 (mouse): S235‑p, S6 (rat): S235‑p, S6 (fruit fly): A234‑p, S6 (cow): S235‑p
Characterization
Methods used to characterize site in vivo 2D analysis, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  PC CL 3 (thyroid cell)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  dog, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
TSH increase

S236-p - S6 (rat)
Modsite: AKRRRLssLRAstsK SwissProt Entrez-Gene
Orthologous residues
S6 (human): S236‑p, S6 (mouse): S236‑p, S6 (rat): S236‑p, S6 (fruit fly): S235‑p, S6 (cow): S236‑p
Characterization
Methods used to characterize site in vivo 2D analysis, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  PC CL 3 (thyroid cell)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  dog, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
TSH increase

T1466-p - TSC2 (rat)
Modsite: GLRPRGYtISDSAPS SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): T1462‑p, TSC2 iso2 (human): T1419‑p, TSC2 iso3 (human): T1418‑p, TSC2 iso4 (human): T1439‑p, TSC2 (mouse): T1465‑p, TSC2 iso6 (mouse): T1443‑p, TSC2 (rat): T1466‑p, TSC2 iso2 (rat): T1423‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  PC CL 3 (thyroid cell)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  dog, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
NKH_477 no change compared to control
TSH no change compared to control

Y1573-p - TSC2 (rat)
Modsite: NEHGSYRyTEFLTGL SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): Y1571‑p, TSC2 iso2 (human): Y1528‑p, TSC2 iso3 (human): Y1527‑p, TSC2 iso4 (human): Y1548‑p, TSC2 (mouse): Y1572‑p, TSC2 iso6 (mouse): Y1550‑p, TSC2 (rat): Y1573‑p, TSC2 iso2 (rat): Y1530‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  PC CL 3 (thyroid cell)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  dog, rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase