Curated Information
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Home > Curated Information Page > PubMed Id: 7889942
Gille H, et al. (1995) ERK phosphorylation potentiates Elk-1-mediated ternary complex formation and transactivation. EMBO J 14, 951-62 7889942
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S325-p - ELK1 (mouse)
Modsite: RDLELPLsPSLLGGQ SwissProt Entrez-Gene
Orthologous residues
ELK1 (human): S324‑p, ELK1 (mouse): S325‑p, ELK1 (rat): S323‑p
Characterization
Methods used to characterize site in vivo 2D analysis, [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK1 (mouse)

T337-p - ELK1 (mouse)
Modsite: GGQGPERtPGSGTSS SwissProt Entrez-Gene
Orthologous residues
ELK1 (human): T336‑p, ELK1 (mouse): T337‑p, ELK1 (rat): T335‑p
Characterization
Methods used to characterize site in vivo 2D analysis, [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK1 (mouse)

S384-p - ELK1 (mouse)
Modsite: IHFWSTLsPIAPRsP SwissProt Entrez-Gene
Orthologous residues
ELK1 (human): S383‑p, ELK1 (mouse): S384‑p, ELK1 (rat): S382‑p
Characterization
Methods used to characterize site in vivo 2D analysis, [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK1 (mouse)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum increase
EGF increase

S390-p - ELK1 (mouse)
Modsite: LsPIAPRsPAKLSFQ SwissProt Entrez-Gene
Orthologous residues
ELK1 (human): S389‑p, ELK1 (mouse): S390‑p, ELK1 (rat): S388‑p
Characterization
Methods used to characterize site in vivo 2D analysis, [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK1 (mouse)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
serum increase
EGF increase

S423-p - ELK1 (mouse)
Modsite: LStPVVLsPGPQKP_ SwissProt Entrez-Gene
Orthologous residues
ELK1 (human): S422‑p, ELK1 (mouse): S423‑p, ELK1 (rat): S421‑p
Characterization
Methods used to characterize site in vivo 2D analysis, [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  3T3 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ERK1 (mouse)