Curated Information
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Home > Curated Information Page > PubMed Id: 21940452
Fang WQ, et al. (2011) Cdk5-mediated phosphorylation of Axin directs axon formation during cerebral cortex development. J Neurosci 31, 13613-24 21940452
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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S473-p - Akt1 (human)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  neuron-'brain, hippocampus'
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
axin 1 (mouse) no change compared to control

T481-p - axin 1 (human)
Modsite: HVQRVLRtPGRQsPG SwissProt Entrez-Gene
Orthologous residues
axin 1 (human): T481‑p, axin 1 iso2 (human): T481‑p, axin 1 (mouse): T480‑p, axin 1 (rat): T480‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  neuron-'brain, hippocampus'
Cellular systems studied:  primary cells
Species studied:  mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK5 (human)

T480-p - axin 1 (mouse)
Modsite: HVQRVMRtPGCQsPG SwissProt Entrez-Gene
Orthologous residues
axin 1 (human): T481‑p, axin 1 iso2 (human): T481‑p, axin 1 (mouse): T480‑p, axin 1 (rat): T480‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  neuron-'brain, hippocampus'
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CDK5 (mouse) phospho-antibody, genetic knockout/knockin of upstream enzyme, pharmacological inhibitor of upstream enzyme, mutation in upstream enzyme recognition motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
wortmannin no change compared to control
BDNF increase
seliciclib BDNF inhibit treatment-induced increase
neurotrophin-3 increase
seliciclib neurotrophin-3 inhibit treatment-induced increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  cytoskeletal reorganization
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
GSK3B (mouse) Induces enzymatic activity, inhibited, intracellular localization cytoskeletal reorganization co-immunoprecipitation
Comments:  axon formation

T514-p - CRMP-2 (mouse)
Modsite: sVtPktVtPAssAkt SwissProt Entrez-Gene
Orthologous residues
CRMP‑2 (human): T514‑p, CRMP‑2 iso3 (human): T619‑p, CRMP‑2 (mouse): T514‑p, CRMP‑2 (rat): T514‑p, CRMP‑2 (chicken): T514‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  neuron-'brain, hippocampus'
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
axin 1 (mouse) decrease axin 1 shRNA increase
axin 1 (mouse) axin 1 (mouse) increase L392P mutant

S9-p - GSK3B (mouse)
Modsite: SGRPRttsFAEsCKP SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S9‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  neuron-'brain, hippocampus'
Cellular systems studied:  primary cells
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
axin 1 (mouse) increase axin 1 shRNA inhibits
axin 1 (mouse) axin 1 (mouse) decrease L392P mutant
wortmannin decrease
BDNF increase
seliciclib BDNF inhibit treatment-induced increase
neurotrophin-3 increase
seliciclib neurotrophin-3 inhibit treatment-induced increase