Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 22158866
Maeno Y, et al. (2012) Inhibition of Insulin Signaling in Endothelial Cells by Protein Kinase C-induced Phosphorylation of p85 Subunit of Phosphatidylinositol 3-Kinase (PI3K). J Biol Chem 287, 4518-30 22158866
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

T202-p - ERK1 (human)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  BAEC (endothelial)
Cellular systems studied:  cell lines
Species studied:  cow
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
phorbol_ester increase
phorbol_ester insulin augment treatment-induced increase
GF109203X no change compared to control
GF109203X insulin no change compared to control
GF109203X GF109203X, phorbol_ester inhibit treatment-induced increase
phorbol_ester, GF109203X insulin decrease

Y204-p - ERK1 (human)
Modsite: HtGFLtEyVAtRWyr SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  BAEC (endothelial)
Cellular systems studied:  cell lines
Species studied:  cow
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
phorbol_ester increase
phorbol_ester insulin augment treatment-induced increase
GF109203X no change compared to control
GF109203X insulin no change compared to control
GF109203X GF109203X, phorbol_ester inhibit treatment-induced increase
phorbol_ester, GF109203X insulin decrease

T185-p - ERK2 (human)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  BAEC (endothelial)
Cellular systems studied:  cell lines
Species studied:  cow
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
phorbol_ester increase
phorbol_ester insulin augment treatment-induced increase
GF109203X no change compared to control
GF109203X insulin no change compared to control
GF109203X GF109203X, phorbol_ester inhibit treatment-induced increase
phorbol_ester, GF109203X insulin decrease

Y187-p - ERK2 (human)
Modsite: HtGFLtEyVAtRWyr SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  BAEC (endothelial)
Cellular systems studied:  cell lines
Species studied:  cow
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
phorbol_ester increase
phorbol_ester insulin augment treatment-induced increase
GF109203X no change compared to control
GF109203X insulin no change compared to control
GF109203X GF109203X, phorbol_ester inhibit treatment-induced increase
phorbol_ester, GF109203X insulin decrease

S302-p - IRS1 (rat)
Modsite: TRRSRTEsITATsPA SwissProt Entrez-Gene
Orthologous residues
IRS1 (human): S307‑p, IRS1 (mouse): S302‑p, IRS1 (rat): S302‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  BAEC (endothelial)
Cellular systems studied:  cell lines
Species studied:  cow
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin no change compared to control

Y608-p - IRS1 (rat)
Modsite: NLHtDDGyMPMsPGV SwissProt Entrez-Gene
Orthologous residues
IRS1 (human): Y612‑p, IRS1 (mouse): Y608‑p, IRS1 (rat): Y608‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  BAEC (endothelial)
Cellular systems studied:  cell lines
Species studied:  cow

S612-p - IRS1 (rat)
Modsite: DDGyMPMsPGVAPVP SwissProt Entrez-Gene
Orthologous residues
IRS1 (human): S616‑p, IRS1 (mouse): S612‑p, IRS1 (rat): S612‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  BAEC (endothelial)
Cellular systems studied:  cell lines
Species studied:  cow
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin no change compared to control

S891-p - IRS1 (rat)
Modsite: LHPPEPKsPGEyVNI SwissProt Entrez-Gene
Orthologous residues
IRS1 (human): S892‑p, IRS1 (mouse): S887‑p, IRS1 (rat): S891‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  BAEC (endothelial)
Cellular systems studied:  cell lines
Species studied:  cow
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin no change compared to control

T308-p - Akt1 (cow)
Modsite: KDGATMKtFCGTPEY SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  BAEC (endothelial)
Cellular systems studied:  cell lines
Species studied:  cow
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
phorbol_ester no change compared to control
phorbol_ester insulin inhibit treatment-induced increase
insulin IRS1 (rat) augment treatment-induced increase
phorbol_ester IRS1 (rat) no change compared to control
insulin IRS1 (rat) inhibit treatment-induced increase

S473-p - Akt1 (cow)
Modsite: RPHFPQFsYSASATA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  BAEC (endothelial)
Cellular systems studied:  cell lines
Species studied:  cow
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
phorbol_ester no change compared to control
phorbol_ester insulin inhibit treatment-induced increase
GF109203X increase
GF109203X insulin augment treatment-induced increase
phorbol_ester GF109203X no effect upon treatment-induced increase
phorbol_ester, GF109203X insulin augment treatment-induced increase
insulin IRS1 (rat) augment treatment-induced increase
phorbol_ester IRS1 (rat) no change compared to control
insulin, phorbol_ester IRS1 (rat) increase
insulin IRS2 (rat) no effect upon treatment-induced increase IRS2 siRNA no effect
IRS2 (rat) no change compared to control IRS2 siRNA no change
phorbol_ester IRS2 (rat) no effect upon treatment-induced decrease IRS2 siRNA no effect
insulin IRS2 (human) no effect upon treatment-induced increase IRS2 -/- no effect
phorbol_ester IRS2 (rat) no change compared to control IRS2 -/- no change
insulin, phorbol_ester IRS2 (rat) no effect upon treatment-induced decrease IRS2 -/- no effect
PIK3CA (human) increase myr-PIK3CA
phorbol_ester PIK3R1 (cow) no effect upon treatment-induced decrease PIK3R1 siRNA no effect (slight increase)
phorbol_ester PIK3R1 (cow) inhibit treatment-induced decrease PIK3R1 siRNA + PIK3R1 deletion mutant
VEGF increase
phorbol_ester VEGF inhibit treatment-induced increase
phorbol_ester PIK3R1 (cow) no effect upon treatment-induced decrease PIK3R1 siRNA-no effect
phorbol_ester PIK3R1 (cow) inhibit treatment-induced decrease PIK3R1 deletion mutant + siRNA
IRS1 (rat) no change compared to control
insulin IRS1 (rat) increase
phorbol_ester insulin IRS1 (rat) inhibit treatment-induced increase
angiotensin IRS1 (rat) decrease
insulin angiotensin IRS1 (rat) no effect upon treatment-induced decrease
GF109203X angiotensin IRS1 (rat) inhibit treatment-induced decrease

S1181-p - eNOS (cow)
Modsite: RIRtQsFsLQERHLR SwissProt Entrez-Gene
Orthologous residues
eNOS (human): S1179‑p, eNOS (mouse): S1178‑p, eNOS (rat): S1178‑p, eNOS (rabbit): S1185‑p, eNOS (pig): S1181‑p, eNOS (cow): S1181‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  BAEC (endothelial)
Cellular systems studied:  cell lines
Species studied:  cow
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin no change compared to control
phorbol_ester no change compared to control
phorbol_ester insulin no change compared to control
insulin IRS1 (rat) increase
phorbol_ester IRS1 (rat) no change compared to control
insulin, phorbol_ester IRS1 (rat) inhibit treatment-induced increase

T86-p - PIK3R1 (cow)
Modsite: RKKIsPPtPKPRPPR SwissProt Entrez-Gene
Orthologous residues
PIK3R1 (human): T86‑p, PIK3R1 iso2 (human): , PIK3R1 iso4 (human): T86‑p, PIK3R1 (mouse): T86‑p, PIK3R1 (rat): T86‑p, PIK3R1 (cow): T86‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  BAEC (endothelial)
Cellular systems studied:  cell lines
Species studied:  cow
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKCA (cow) pharmacological inhibitor of upstream enzyme, pharmacological activator of upstream enzyme, phospho-antibody
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
phorbol_ester increase
GF109203X inhibit treatment-induced increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
IRS1 (rat) Disrupts activity, inhibited co-immunoprecipitation