Ma P, et al. (2012) A newly identified complex of spinophilin and the tyrosine phosphatase, SHP-1, modulates platelet activation by regulating G protein-dependent signaling. Blood 119, 1935-45 22210881

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Y536-p - SHP-1 (mouse) ▲

Modsite: QKGQEsEyGNItyPP SwissProt Entrez-Gene Orthologous residues SHP‑1 (human): Y536‑p, SHP‑1 iso2 (human): Y497‑p, SHP‑1 iso3 (human): Y538‑p, SHP‑1 iso4 (human): Y536‑p, SHP‑1 (mouse): Y536‑p, SHP‑1 (rat): Y538‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  CHO (fibroblast), platelet-blood Cellular systems studied:  cell lines, primary cultured cells Species studied:  hamster, human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes thrombin increase PP2 thrombin inhibit treatment-induced increase Downstream Regulation Effect of modification (function):  enzymatic activity, induced

S591-p - SHP-1 (mouse) ▲
Modsite: DKEKNKGsLKRK___ SwissProt Entrez-Gene Orthologous residues SHP‑1 (human): S591‑p, SHP‑1 iso2 (human): S552‑p, SHP‑1 iso3 (human): S593‑p, SHP‑1 iso4 (human): ‑, SHP‑1 (mouse): S591‑p, SHP‑1 (rat): S593‑p

Y398-p - Spinophilin (mouse) ▲

Modsite: DLVDVSAySGLGEDS SwissProt Entrez-Gene Orthologous residues Spinophilin (human): Y398‑p, Spinophilin (mouse): Y398‑p, Spinophilin (rat): Y398‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  CHO (fibroblast), platelet-blood Cellular systems studied:  cell lines, primary cultured cells Species studied:  hamster, human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes PAR1-activating_peptide decrease U46619 decrease NSC-87877 PAR1-activating_peptide inhibit treatment-induced decrease SHP-1 (human) decrease thrombin SHP-1 (human) augment treatment-induced decrease Downstream Regulation Effect of modification (function):  molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays SHP-1 (human) Induces co-immunoprecipitation

Y483-p - Spinophilin (mouse) ▲

Modsite: PMAASAEyELEKRVE SwissProt Entrez-Gene Orthologous residues Spinophilin (human): Y483‑p, Spinophilin (mouse): Y483‑p, Spinophilin (rat): Y483‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  CHO (fibroblast), platelet-blood Cellular systems studied:  cell lines, primary cultured cells Species studied:  hamster, human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes PAR1-activating_peptide decrease U46619 decrease NSC-87877 PAR1-activating_peptide inhibit treatment-induced decrease SHP-1 (human) decrease thrombin SHP-1 (human) augment treatment-induced decrease