Curated Information
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Home > Curated Information Page > Cst Curation Set #13061
Mulhern D (2011) CST Curation Set: 13061; Year: 2011; Biosample/Treatment: cell line, HCT116/untreated; Disease: colorectal carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: m2K
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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K116-m2 - Calmodulin (human)
Modsite: VMtNLGEkLtDEEVD SwissProt Entrez-Gene
Orthologous residues
Calmodulin (human): K116‑m2, Calmodulin (mouse): K116‑m2, Calmodulin (rat): K116‑m2, Calmodulin (chicken): K116‑m2, Calmodulin (sheep): K116‑m2, Calmodulin (cow): K116‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K64-m2 - CRIP1 (human)
Modsite: yAAMFGPkGFGrGGA SwissProt Entrez-Gene
Orthologous residues
CRIP1 (human): K64‑m2, CRIP1 (mouse): K64‑m2, CRIP1 (rat): K64‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K55-m2 - EEF1A1 (human)
Modsite: EMGkGSFkyAWVLDk SwissProt Entrez-Gene
Orthologous residues
EEF1A1 (human): K55‑m2, EEF1A1 (mouse): K55‑m2, EEF1A1 (rat): K55‑m2, EEF1A1 (rabbit): K55‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K79-m2 - EEF1A1 (human)
Modsite: TIDIsLWkFEtSkyy SwissProt Entrez-Gene
Orthologous residues
EEF1A1 (human): K79‑m2, EEF1A1 (mouse): K79‑m2, EEF1A1 (rat): K79‑m2, EEF1A1 (rabbit): K79‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K84-m2 - EEF1A1 (human)
Modsite: LWkFEtSkyyVtIID SwissProt Entrez-Gene
Orthologous residues
EEF1A1 (human): K84‑m2, EEF1A1 (mouse): K84‑m2, EEF1A1 (rat): K84‑m2, EEF1A1 (rabbit): K84‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K165-m2 - EEF1A1 (human)
Modsite: tEPPysQkryEEIVk SwissProt Entrez-Gene
Orthologous residues
EEF1A1 (human): K165‑m2, EEF1A1 (mouse): K165‑m2, EEF1A1 (rat): K165‑m2, EEF1A1 (rabbit): K165‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K318-m2 - EEF1A1 (human)
Modsite: NVkNVsVkDVRRGNV SwissProt Entrez-Gene
Orthologous residues
EEF1A1 (human): K318‑m2, EEF1A1 (mouse): K318‑m2, EEF1A1 (rat): K318‑m2, EEF1A1 (rabbit): K318‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K55-m2 - EEF1A2 (human)
Modsite: EMGkGSFkyAWVLDk SwissProt Entrez-Gene
Orthologous residues
EEF1A2 (human): K55‑m2, EEF1A2 (mouse): K55‑m2, EEF1A2 (rat): K55‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K79-m2 - EEF1A2 (human)
Modsite: TIDISLWkFETTkyy SwissProt Entrez-Gene
Orthologous residues
EEF1A2 (human): K79‑m2, EEF1A2 (mouse): K79‑m2, EEF1A2 (rat): K79‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K165-m2 - EEF1A2 (human)
Modsite: TEPAYSEkrYDEIVk SwissProt Entrez-Gene
Orthologous residues
EEF1A2 (human): K165‑m2, EEF1A2 (mouse): K165‑m2, EEF1A2 (rat): K165‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K318-m2 - EEF1A2 (human)
Modsite: NVkNVSVkDIRRGNV SwissProt Entrez-Gene
Orthologous residues
EEF1A2 (human): K318‑m2, EEF1A2 (mouse): K318‑m2, EEF1A2 (rat): K318‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K525-m2 - EEF2 (human)
Modsite: PkLVEGLkrLAksDP SwissProt Entrez-Gene
Orthologous residues
EEF2 (human): K525‑m2, EEF2 (mouse): K525‑m2, EEF2 (rat): K525‑m2, EEF2 (rabbit): K524‑m2, EEF2 (cow): K525‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K505-m2 - EZH2 (human)
Modsite: LWAAHCRkIQLkkDG SwissProt Entrez-Gene
Orthologous residues
EZH2 (human): K505‑m2, EZH2 iso2 (human): K510‑m2, EZH2 (mouse): K505‑m2, EZH2 (rat): K505‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K510-m2 - EZH2 (human)
Modsite: CRkIQLkkDGSSNHV SwissProt Entrez-Gene
Orthologous residues
EZH2 (human): K510‑m2, EZH2 iso2 (human): K515‑m2, EZH2 (mouse): K510‑m2, EZH2 (rat): K510‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K585-m2 - GRP78 (human)
Modsite: DkEkLGGkLssEDkE SwissProt Entrez-Gene
Orthologous residues
GRP78 (human): K585‑m2, GRP78 (mouse): K586‑m2, GRP78 (rat): K585‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K27-m2 - H3 (human)
Modsite: LATkAArksAPATGG SwissProt Entrez-Gene
Orthologous residues
H3 (human): K27‑m2, H3 (mouse): K27‑m2, H3 iso2 (mouse): K27‑m2, H3 iso3 (mouse): K27‑m2, H3 (rat): K27‑m2, H3 iso3 (rat): K27‑m2, H3 (pig): K27‑m2, H3 (chicken): K27‑m2, H3 (cow): K27‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K36-m2 - H3 (human)
Modsite: APATGGVkkPHryrP SwissProt Entrez-Gene
Orthologous residues
H3 (human): K36‑m2, H3 (mouse): K36‑m2, H3 iso2 (mouse): K36‑m2, H3 iso3 (mouse): K36‑m2, H3 (rat): K36‑m2, H3 iso3 (rat): K36‑m2, H3 (pig): K36‑m2, H3 (chicken): K36‑m2, H3 (cow): K36‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K37-m2 - H3 (human)
Modsite: PATGGVkkPHryrPG SwissProt Entrez-Gene
Orthologous residues
H3 (human): K37‑m2, H3 (mouse): K37‑m2, H3 iso2 (mouse): K37‑m2, H3 iso3 (mouse): K37‑m2, H3 (rat): K37‑m2, H3 iso3 (rat): K37‑m2, H3 (pig): K37‑m2, H3 (chicken): K37‑m2, H3 (cow): K37‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K79-m2 - H3 (human)
Modsite: REIAQDFktDLrFQs SwissProt Entrez-Gene
Orthologous residues
H3 (human): K79‑m2, H3 (mouse): K79‑m2, H3 iso2 (mouse): K79‑m2, H3 iso3 (mouse): K79‑m2, H3 (rat): K79‑m2, H3 iso3 (rat): K79‑m2, H3 (pig): K79‑m2, H3 (chicken): K79‑m2, H3 (cow): K79‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K27-m2 - H3F3A (human)
Modsite: LATkAARksAPsTGG SwissProt Entrez-Gene
Orthologous residues
H3F3A (human): K27‑m2, H3F3A (mouse): K27‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K36-m2 - H3F3A (human)
Modsite: APsTGGVkkPHryrP SwissProt Entrez-Gene
Orthologous residues
H3F3A (human): K36‑m2, H3F3A (mouse): K36‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K37-m2 - H3F3A (human)
Modsite: PsTGGVkkPHryrPG SwissProt Entrez-Gene
Orthologous residues
H3F3A (human): K37‑m2, H3F3A (mouse): K37‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K79-m2 - H3F3A (human)
Modsite: REIAQDFktDLrFQs SwissProt Entrez-Gene
Orthologous residues
H3F3A (human): K79‑m2, H3F3A (mouse): K79‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K80-m2 - HIST2H3A/C/D (human)
Modsite: REIAQDFktDLrFQS SwissProt Entrez-Gene
Orthologous residues
HIST2H3A/C/D (human): K80‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K28-m2 - HIST3H3 (human)
Modsite: LATkVARksAPAtGG SwissProt Entrez-Gene
Orthologous residues
HIST3H3 (human): K28‑m2, HIST3H3 (mouse): K28‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K37-m2 - HIST3H3 (human)
Modsite: APAtGGVkkPHryrP SwissProt Entrez-Gene
Orthologous residues
HIST3H3 (human): K37‑m2, HIST3H3 (mouse): K37‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K38-m2 - HIST3H3 (human)
Modsite: PAtGGVkkPHryrPG SwissProt Entrez-Gene
Orthologous residues
HIST3H3 (human): K38‑m2, HIST3H3 (mouse): K38‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K561-m2 - HSC70 (human)
Modsite: EDEkLQGkINDEDkQ SwissProt Entrez-Gene
Orthologous residues
HSC70 (human): K561‑m2, HSC70 iso2 (human): , HSC70 (mouse): K561‑m2, HSC70 (rat): K561‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K561-m2 - HSP70 (human)
Modsite: EDEGLkGkISEADkk SwissProt Entrez-Gene
Orthologous residues
HSP70 (human): K561‑m2, HSP70 iso2 (human): K506‑m2, HSP70 (mouse): K561‑m2, HSP70 (rat): K561‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K1214-m2 - LCOR iso3 (human)
Modsite: PGDVPPVkHPLQkYA SwissProt Entrez-Gene
Orthologous residues
LCOR (human): , LCOR iso3 (human): K1214‑m2, LCOR (mouse):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K1219-m2 - LCOR iso3 (human)
Modsite: PVkHPLQkYAPSSLY SwissProt Entrez-Gene
Orthologous residues
LCOR (human): , LCOR iso3 (human): K1219‑m2, LCOR (mouse):
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K1425-m2 - PRPF8 (human)
Modsite: rINTLFQkDRHTLAy SwissProt Entrez-Gene
Orthologous residues
PRPF8 (human): K1425‑m2, PRPF8 (mouse): K1425‑m2, PRPF8 (rat): K1425‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K1170-m2 - SETDB1 (human)
Modsite: sTRGFALkSTHGIAI SwissProt Entrez-Gene
Orthologous residues
SETDB1 (human): K1170‑m2, SETDB1 iso3 (human): K1170‑m2, SETDB1 (mouse): K1186‑m2, SETDB1 (rat): K1181‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K1178-m2 - SETDB1 (human)
Modsite: STHGIAIkStNMAsV SwissProt Entrez-Gene
Orthologous residues
SETDB1 (human): K1178‑m2, SETDB1 iso3 (human): K1178‑m2, SETDB1 (mouse): K1194‑m2, SETDB1 (rat): K1189‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K52-m2 - SLC25A4 (human)
Modsite: ISAEKQYkGIIDCVV SwissProt Entrez-Gene
Orthologous residues
SLC25A4 (human): K52‑m2, SLC25A4 (mouse): K52‑m2, SLC25A4 (rat): K52‑m2, SLC25A4 (pig): K53‑m2, SLC25A4 (cow): K52‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K52-m2 - SLC25A5 (human)
Modsite: ITADkQYkGIIDCVV SwissProt Entrez-Gene
Orthologous residues
SLC25A5 (human): K52‑m2, SLC25A5 (mouse): K52‑m2, SLC25A5 (rat): K52‑m2, SLC25A5 (pig): K52‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K315-m2 - VCP (human)
Modsite: AIAPkREktHGEVER SwissProt Entrez-Gene
Orthologous residues
VCP (human): K315‑m2, VCP (mouse): K315‑m2, VCP (rat): K315‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K238-m2 - ZNF326 (human)
Modsite: VAAArGIkrkMMQPF SwissProt Entrez-Gene
Orthologous residues
ZNF326 (human): K238‑m2, ZNF326 iso3 (human): K32‑m2, ZNF326 (mouse): K238‑m2, ZNF326 (rat): K217‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody

K26-m2 - ZNF364 (human)
Modsite: RFFCHFCkGEVSPkL SwissProt Entrez-Gene
Orthologous residues
ZNF364 (human): K26‑m2, ZNF364 (mouse): K26‑m2
Characterization
Methods used to characterize site in vivo mass spectrometry
Relevant cell lines - cell types - tissues:  HCT116 (intestinal)
Cellular systems studied:  cell lines
Species studied:  human
Comments:  di-methyl lysine antibody