Tucker M (2009) CST Curation Set: 6046; Year: 2009; Biosample/Treatment: tissue, skin/untreated; Disease: melanoma skin cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb Cat#: 10001, PTMScan(R) Phospho-Akt Substrate Motif (RXRXXS*/T*) Immunoaffinity Beads Cat#: 1979

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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S5642-p - AHNAK2 (human) ▲

Modsite: DKPESKKsGLLWFWL SwissProt Entrez-Gene Orthologous residues AHNAK2 (human): S5642‑p, AHNAK2 iso2 (human): S640‑p, AHNAK2 iso3 (human): S5542‑p, AHNAK2 (mouse): ‑, AHNAK2 iso2 (mouse): A623‑p, AHNAK2 (rat): S6885‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

S272-p - amphiphysin (human) ▲
Modsite: EEPsPLPsPTAsPNH SwissProt Entrez-Gene Orthologous residues amphiphysin (human): S272‑p, amphiphysin (mouse): S272‑p, amphiphysin (rat): S272‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

S293-p - amphiphysin (human) ▲
Modsite: PAPARPRsPsQTRKG SwissProt Entrez-Gene Orthologous residues amphiphysin (human): S293‑p, amphiphysin (mouse): S293‑p, amphiphysin (rat): S293‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

Y15-p - CDK1 (human) ▲

Modsite: EkIGEGtyGVVykGR SwissProt Entrez-Gene Orthologous residues CDK1 (human): Y15‑p, CDK1 iso2 (human): Y15‑p, CDK1 (mouse): Y15‑p, CDK1 (rat): Y15‑p, CDK1 (chicken): Y15‑p, CDK1 (fruit fly): Y15‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

Y15-p - CDK2 (human) ▲
Modsite: EkIGEGtyGVVykAR SwissProt Entrez-Gene Orthologous residues CDK2 (human): Y15‑p, CDK2 iso2 (human): Y15‑p, CDK2 (mouse): Y15‑p, CDK2 (rat): Y15‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

Y15-p - CDK3 (human) ▲
Modsite: EkIGEGtyGVVyKAK SwissProt Entrez-Gene Orthologous residues CDK3 (human): Y15‑p, CDK3 (mouse): Y15‑p, CDK3 (cow): Y15‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

Y279-p - GSK3A (human) ▲
Modsite: RGEPNVsyICsRyyR SwissProt Entrez-Gene Orthologous residues GSK3A (human): Y279‑p, GSK3A (mouse): Y279‑p, GSK3A (rat): Y279‑p, GSK3A (cow): Y279‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

Y216-p - GSK3B (human) ▲

Modsite: RGEPNVsyICsRyyR SwissProt Entrez-Gene Orthologous residues GSK3B (human): Y216‑p, GSK3B iso2 (human): Y216‑p, GSK3B (mouse): Y216‑p, GSK3B (rat): Y216‑p, GSK3B (rabbit): Y216‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

T257-p - LARP7 (human) ▲

Modsite: KMKRsRPtsEGsDIE SwissProt Entrez-Gene Orthologous residues LARP7 (human): T257‑p, LARP7 iso2 (human): ‑, LARP7 iso3 (human): T264‑p, LARP7 (mouse): A252‑p, LARP7 (rat): A253‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

S258-p - LARP7 (human) ▲

Modsite: MKRsRPtsEGsDIEs SwissProt Entrez-Gene Orthologous residues LARP7 (human): S258‑p, LARP7 iso2 (human): ‑, LARP7 iso3 (human): S265‑p, LARP7 (mouse): S253‑p, LARP7 (rat): S254‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

S261-p - LARP7 (human) ▲

Modsite: sRPtsEGsDIEstEP SwissProt Entrez-Gene Orthologous residues LARP7 (human): S261‑p, LARP7 iso2 (human): ‑, LARP7 iso3 (human): S268‑p, LARP7 (mouse): S256‑p, LARP7 (rat): S257‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

S802-p - MYPT1 (human) ▲

Modsite: sQLNRPNsLVGITSA SwissProt Entrez-Gene Orthologous residues MYPT1 (human): S802‑p, MYPT1 iso2 (human): S802‑p, MYPT1 iso3 (human): S746‑p, MYPT1 iso4 (human): S743‑p, MYPT1 (mouse): S801‑p, MYPT1 (rat): S804‑p, MYPT1 (pig): S802‑p, MYPT1 (chicken): S800‑p, MYPT1 (cow): S800‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

S271-p - NEURL1B (human) ▲

Modsite: RERPRPAssPALLEA SwissProt Entrez-Gene Orthologous residues NEURL1B (human): S271‑p, NEURL1B iso2 (human): S89‑p, NEURL1B iso3 (human): ‑, NEURL1B (mouse): S262‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

Y182-p - P38A (human) ▲

Modsite: tDDEMtGyVAtRWYR SwissProt Entrez-Gene Orthologous residues P38A (human): Y182‑p, P38A iso2 (human): Y182‑p, P38A (mouse): Y182‑p, P38A iso3 (mouse): Y182‑p, P38A (rat): Y182‑p, P38A (salmonid): Y183‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

Y849-p - PRP4 (human) ▲
Modsite: ADNDItPyLVsRFyR SwissProt Entrez-Gene Orthologous residues PRP4 (human): Y849‑p, PRP4 (mouse): Y849‑p, PRP4 (rat): Y849‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

T112-p - Pumilio-1 (human) ▲

Modsite: NskHRWPtGDNIHAE SwissProt Entrez-Gene Orthologous residues Pumilio‑1 (human): T112‑p, Pumilio‑1 iso2 (human): T112‑p, Pumilio‑1 iso3 (human): T112‑p, Pumilio‑1 iso4 (human): T148‑p, Pumilio‑1 (mouse): T112‑p, Pumilio‑1 (rat): T112‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

S754-p - SCYL1 (human) ▲
Modsite: sTQPRPDsWGEDNWE SwissProt Entrez-Gene Orthologous residues SCYL1 (human): S754‑p, SCYL1 iso2 (human): S737‑p, SCYL1 (mouse): S752‑p, SCYL1 (rat): S753‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

S297-p - SPRED2 (human) ▲
Modsite: TQPSRGKsRRRKEDG SwissProt Entrez-Gene Orthologous residues SPRED2 (human): S297‑p, SPRED2 iso2 (human): S294‑p, SPRED2 (mouse): S289‑p, SPRED2 (rat): S289‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

S297-p - SRm300 (human) ▲

Modsite: ALAGRsPsPAsGRRG SwissProt Entrez-Gene Orthologous residues SRm300 (human): S297‑p, SRm300 iso2 (human): S297‑p, SRm300 (mouse): P297‑p, SRm300 iso2 (mouse): P201‑p, SRm300 iso3 (mouse): P297‑p, SRm300 (rat): P297‑p, SRm300 (cow): S297‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

T1080-p - VIRMA (human) ▲
Modsite: QGVNEKLtISEETLA SwissProt Entrez-Gene Orthologous residues VIRMA (human): T1080‑p, VIRMA (mouse): T1079‑p, VIRMA (rat): T1130‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human