Tucker M (2009) CST Curation Set: 6044; Year: 2009; Biosample/Treatment: tissue, skin/untreated; Disease: melanoma skin cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Akt Substrate (RXRXXS*/T*) (23C8D2) Rabbit mAb Cat#: 10001, PTMScan(R) Phospho-Akt Substrate Motif (RXRXXS*/T*) Immunoaffinity Beads Cat#: 1979

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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S121-p - ARHGEF2 (human) ▲

Modsite: TtIRERPssAIyPsD SwissProt Entrez-Gene Orthologous residues ARHGEF2 (human): S121‑p, ARHGEF2 iso2 (human): S121‑p, ARHGEF2 iso3 (human): S94‑p, ARHGEF2 (mouse): T121‑p, ARHGEF2 (rat): T121‑p, ARHGEF2 (pig): S94‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

Y321-p - DYRK1A (human) ▲

Modsite: LGQRIyQyIQsRFYR SwissProt Entrez-Gene Orthologous residues DYRK1A (human): Y321‑p, DYRK1A iso2 (human): Y312‑p, DYRK1A iso4 (human): Y321‑p, DYRK1A (mouse): Y321‑p, DYRK1A (rat): Y321‑p, DYRK1A (rabbit): ‑ Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

Y273-p - DYRK1B (human) ▲
Modsite: LGQRIyQyIQsRFYR SwissProt Entrez-Gene Orthologous residues DYRK1B (human): Y273‑p, DYRK1B iso2 (human): Y273‑p, DYRK1B (mouse): Y273‑p, DYRK1B (rat): Y273‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

Y204-p - ERK1 (human) ▲

Modsite: HtGFLtEyVAtRWyr SwissProt Entrez-Gene Orthologous residues ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

T185-p - ERK2 (human) ▲

Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene Orthologous residues ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

Y187-p - ERK2 (human) ▲

Modsite: HtGFLtEyVAtRWyr SwissProt Entrez-Gene Orthologous residues ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

Y279-p - GSK3A (human) ▲
Modsite: RGEPNVsyICsRyyR SwissProt Entrez-Gene Orthologous residues GSK3A (human): Y279‑p, GSK3A (mouse): Y279‑p, GSK3A (rat): Y279‑p, GSK3A (cow): Y279‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

Y216-p - GSK3B (human) ▲

Modsite: RGEPNVsyICsRyyR SwissProt Entrez-Gene Orthologous residues GSK3B (human): Y216‑p, GSK3B iso2 (human): Y216‑p, GSK3B (mouse): Y216‑p, GSK3B (rat): Y216‑p, GSK3B (rabbit): Y216‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

T257-p - LARP7 (human) ▲

Modsite: KMKRsRPtsEGsDIE SwissProt Entrez-Gene Orthologous residues LARP7 (human): T257‑p, LARP7 iso2 (human): ‑, LARP7 iso3 (human): T264‑p, LARP7 (mouse): A252‑p, LARP7 (rat): A253‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

S258-p - LARP7 (human) ▲

Modsite: MKRsRPtsEGsDIEs SwissProt Entrez-Gene Orthologous residues LARP7 (human): S258‑p, LARP7 iso2 (human): ‑, LARP7 iso3 (human): S265‑p, LARP7 (mouse): S253‑p, LARP7 (rat): S254‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

S261-p - LARP7 (human) ▲

Modsite: sRPtsEGsDIEstEP SwissProt Entrez-Gene Orthologous residues LARP7 (human): S261‑p, LARP7 iso2 (human): ‑, LARP7 iso3 (human): S268‑p, LARP7 (mouse): S256‑p, LARP7 (rat): S257‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

S506-p - LRCH4 (human) ▲
Modsite: GsIQRPNsFLFRsss SwissProt Entrez-Gene Orthologous residues LRCH4 (human): S506‑p, LRCH4 (mouse): S506‑p, LRCH4 (rat): S509‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

S237-p - Mnk1 (human) ▲
Modsite: ICDFDLGsGMKLNNS SwissProt Entrez-Gene Orthologous residues Mnk1 (human): S237‑p, Mnk1 iso2 (human): S196‑p, Mnk1 (mouse): S196‑p, Mnk1 (rat): S184‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

Y182-p - P38A (human) ▲

Modsite: tDDEMtGyVAtRWYR SwissProt Entrez-Gene Orthologous residues P38A (human): Y182‑p, P38A iso2 (human): Y182‑p, P38A (mouse): Y182‑p, P38A iso3 (mouse): Y182‑p, P38A (rat): Y182‑p, P38A (salmonid): Y183‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

Y849-p - PRP4 (human) ▲
Modsite: ADNDItPyLVsRFyR SwissProt Entrez-Gene Orthologous residues PRP4 (human): Y849‑p, PRP4 (mouse): Y849‑p, PRP4 (rat): Y849‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

T112-p - Pumilio-1 (human) ▲

Modsite: NskHRWPtGDNIHAE SwissProt Entrez-Gene Orthologous residues Pumilio‑1 (human): T112‑p, Pumilio‑1 iso2 (human): T112‑p, Pumilio‑1 iso3 (human): T112‑p, Pumilio‑1 iso4 (human): T148‑p, Pumilio‑1 (mouse): T112‑p, Pumilio‑1 (rat): T112‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

S754-p - SCYL1 (human) ▲
Modsite: sTQPRPDsWGEDNWE SwissProt Entrez-Gene Orthologous residues SCYL1 (human): S754‑p, SCYL1 iso2 (human): S737‑p, SCYL1 (mouse): S752‑p, SCYL1 (rat): S753‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

S189-p - SPAG6 (human) ▲
Modsite: ALKRIAAsALSDIAK SwissProt Entrez-Gene Orthologous residues SPAG6 (human): S189‑p, SPAG6 (mouse): S189‑p, SPAG6 (rat): S189‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

Y705-p - STAT3 (human) ▲

Modsite: DPGsAAPyLktkFIC SwissProt Entrez-Gene Orthologous residues STAT3 (human): Y705‑p, STAT3 iso2 (human): Y704‑p, STAT3 iso3 (human): Y705‑p, STAT3 (mouse): Y705‑p, STAT3 iso2 (mouse): Y705‑p, STAT3 iso3 (mouse): Y704‑p, STAT3 (rat): Y705‑p, STAT3 (cow): Y705‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human

S121-p - VIPAS39 (human) ▲

Modsite: rGRtrPGsFQsLsDA SwissProt Entrez-Gene Orthologous residues VIPAS39 (human): S121‑p, VIPAS39 iso2 (human): S72‑p, VIPAS39 (mouse): S119‑p, VIPAS39 (rat): S119‑p Characterization Methods used to characterize site in vivo:  mass spectrometry Disease tissue studied:  melanoma skin cancer Relevant cell lines - cell types - tissues:  skin Cellular systems studied:  tissue Species studied:  human