Curated Information
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Home > Curated Information Page > PubMed Id: 21908610
Dickinson RJ, et al. (2011) Phosphorylation of the kinase interaction motif in mitogen-activated protein (MAP) kinase phosphatase-4 mediates cross-talk between protein kinase A and MAP kinase signaling pathways. J Biol Chem 286, 38018-26 21908610
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T202-p - ERK1 (human)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p

Y204-p - ERK1 (human)
Modsite: HtGFLtEyVAtRWyr SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p

T185-p - ERK2 (human)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p

Y187-p - ERK2 (human)
Modsite: HtGFLtEyVAtRWyr SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p

T180-p - P38A (human)
Modsite: RHtDDEMtGyVAtRW SwissProt Entrez-Gene
Orthologous residues
P38A (human): T180‑p, P38A iso2 (human): T180‑p, P38A (mouse): T180‑p, P38A iso3 (mouse): T180‑p, P38A (rat): T180‑p, P38A (salmonid): T181‑p

Y182-p - P38A (human)
Modsite: tDDEMtGyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
P38A (human): Y182‑p, P38A iso2 (human): Y182‑p, P38A (mouse): Y182‑p, P38A iso3 (mouse): Y182‑p, P38A (rat): Y182‑p, P38A (salmonid): Y183‑p

S63-p - ATF-1 (mouse)
Modsite: GILARRPsYRKILKD SwissProt Entrez-Gene
Orthologous residues
ATF‑1 (human): S63‑p, ATF‑1 (mouse): S63‑p

S133-p - CREB (mouse)
Modsite: EILsRRPsyRkILND SwissProt Entrez-Gene
Orthologous residues
CREB (human): S133‑p, CREB iso2 (human): S119‑p, CREB (mouse): S133‑p, CREB (rat): S133‑p

S58-p - DUSP9 (mouse)
Modsite: LRRLRRGsMSVRSLL SwissProt Entrez-Gene
Orthologous residues
DUSP9 (human): S58‑p, DUSP9 (mouse): S58‑p
Characterization
Methods used to characterize site in vivo [32P] ATP in vitro, immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast), E.coli (bacterial), MEF (fibroblast), placenta
Cellular systems studied:  cell lines, tissue
Species studied:  bacteria, green monkey, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKACA (mouse)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKACA (mouse) pharmacological activator of upstream enzyme, transfection of constitutively active enzyme, modification site within consensus motif
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
NKH_477 increase
okadaic_acid NKH_477 augment treatment-induced increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, inhibited, molecular association, regulation
Effect of modification (process):  signaling pathway regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
ERK2 (human) Disrupts phosphorylation, enzymatic activity, induced in vitro, pull-down assay, yeast two-hybrid
P38A (human) Disrupts phosphorylation, enzymatic activity, induced in vitro, pull-down assay, yeast two-hybrid
Comments:  regulates MAPK signaling pathway