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Home > Curated Information Page > PubMed Id: 21868363
Hatziapostolou M, et al. (2011) Tumor progression locus 2 mediates signal-induced increases in cytoplasmic calcium and cell migration. Sci Signal 4, ra55 21868363
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T203-p - ERK1 (mouse)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
thrombin increase
PTX thrombin inhibit treatment-induced increase
Cot (human) increase
thrombin G-alpha i2 (human) augment treatment-induced increase siRNA inhibits
SGPL1 (mouse) Cot (human) augment treatment-induced increase
thrombin increase
GF109203X thrombin inhibit treatment-induced increase
thrombin G-beta 1 (mouse) augment treatment-induced increase siRNA inhibits

Y205-p - ERK1 (mouse)
Modsite: HtGFLtEyVAtRWyR SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
thrombin increase
PTX thrombin inhibit treatment-induced increase
Cot (human) increase
thrombin G-alpha i2 (human) augment treatment-induced increase siRNA inhibits
SGPL1 (mouse) Cot (human) augment treatment-induced increase
thrombin increase
GF109203X thrombin inhibit treatment-induced increase
thrombin G-beta 1 (mouse) augment treatment-induced increase siRNA inhibits

T183-p - ERK2 (mouse)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
thrombin increase
PTX thrombin inhibit treatment-induced increase
Cot (human) increase
thrombin G-alpha i2 (human) augment treatment-induced increase siRNA inhibits
SGPL1 (mouse) Cot (human) augment treatment-induced increase
thrombin increase
GF109203X thrombin inhibit treatment-induced increase
thrombin G-beta 1 (mouse) augment treatment-induced increase siRNA inhibits

Y185-p - ERK2 (mouse)
Modsite: HtGFLtEyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
thrombin increase
PTX thrombin inhibit treatment-induced increase
Cot (human) increase
thrombin G-alpha i2 (human) augment treatment-induced increase siRNA inhibits
SGPL1 (mouse) Cot (human) augment treatment-induced increase
thrombin increase
GF109203X thrombin inhibit treatment-induced increase
thrombin G-beta 1 (mouse) augment treatment-induced increase siRNA inhibits

T183-p - JNK1 (mouse)
Modsite: AGtsFMMtPyVVtRY SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): T183‑p, JNK1 iso2 (human): T183‑p, JNK1 iso3 (human): T183‑p, JNK1 (mouse): T183‑p, JNK1 (rat): T183‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
thrombin increase
PTX thrombin inhibit treatment-induced increase
Cot (human) increase
thrombin G-alpha i2 (human) augment treatment-induced increase siRNA inhibits
SGPL1 (mouse) Cot (human) augment treatment-induced increase

Y185-p - JNK1 (mouse)
Modsite: tsFMMtPyVVtRYYR SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): Y185‑p, JNK1 iso2 (human): Y185‑p, JNK1 iso3 (human): Y185‑p, JNK1 (mouse): Y185‑p, JNK1 (rat): Y185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
thrombin increase
PTX thrombin inhibit treatment-induced increase
Cot (human) increase
thrombin G-alpha i2 (human) augment treatment-induced increase siRNA inhibits
SGPL1 (mouse) Cot (human) augment treatment-induced increase

T183-p - JNK2 (mouse)
Modsite: ACTNFMMtPyVVtRY SwissProt Entrez-Gene
Orthologous residues
JNK2 (human): T183‑p, JNK2 iso2 (human): T183‑p, JNK2 iso3 (human): T183‑p, JNK2 (mouse): T183‑p, JNK2 (rat): T183‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
thrombin increase
PTX thrombin inhibit treatment-induced increase
Cot (human) increase
thrombin G-alpha i2 (human) augment treatment-induced increase siRNA inhibits
SGPL1 (mouse) Cot (human) augment treatment-induced increase

Y185-p - JNK2 (mouse)
Modsite: TNFMMtPyVVtRYYR SwissProt Entrez-Gene
Orthologous residues
JNK2 (human): Y185‑p, JNK2 iso2 (human): Y185‑p, JNK2 iso3 (human): Y185‑p, JNK2 (mouse): Y185‑p, JNK2 (rat): Y185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
thrombin increase
PTX thrombin inhibit treatment-induced increase
Cot (human) increase
thrombin G-alpha i2 (human) augment treatment-induced increase siRNA inhibits
SGPL1 (mouse) Cot (human) augment treatment-induced increase

T638-p - PKCA (mouse)
Modsite: TRGQPVLtPPDQLVI SwissProt Entrez-Gene
Orthologous residues
PKCA (human): T638‑p, PKCA (mouse): T638‑p, PKCA (rat): T638‑p, PKCA (cow): T638‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
thrombin increase
PTX thrombin inhibit treatment-induced increase
thrombin G-alpha i2 (mouse) augment treatment-induced increase siRNA inhibits

S657-p - PKCA (mouse)
Modsite: QsDFEGFsyVNPQFV SwissProt Entrez-Gene
Orthologous residues
PKCA (human): S657‑p, PKCA (mouse): S657‑p, PKCA (rat): S657‑p, PKCA (cow): S657‑p

T642-p - PKCB (mouse)
Modsite: TRQPVELtPTDKLFI SwissProt Entrez-Gene
Orthologous residues
PKCB (human): T642‑p, PKCB iso2 (human): , PKCB (mouse): T642‑p, PKCB iso2 (mouse): , PKCB (rat): T642‑p, PKCB iso2 (rat): , PKCB (cow):
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
thrombin increase
thrombin increase
PTX thrombin inhibit treatment-induced increase
thrombin G-alpha i2 (mouse) augment treatment-induced increase siRNA inhibits

S661-p - PKCB (mouse)
Modsite: QNEFAGFsyTNPEFV SwissProt Entrez-Gene
Orthologous residues
PKCB (human): S661‑p, PKCB iso2 (human): , PKCB (mouse): S661‑p, PKCB iso2 (mouse): , PKCB (rat): S661‑p, PKCB iso2 (rat): , PKCB (cow):

T505-p - PKCD (mouse)
Modsite: FGEGRAstFCGtPDY SwissProt Entrez-Gene
Orthologous residues
PKCD (human): T507‑p, PKCD iso2 (human): T538‑p, PKCD (mouse): T505‑p, PKCD iso2 (mouse): T531‑p, PKCD (rat): T505‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
thrombin increase

S643-p - PKCD (mouse)
Modsite: LNEKPQLsFsDKNLI SwissProt Entrez-Gene
Orthologous residues
PKCD (human): S645‑p, PKCD iso2 (human): S676‑p, PKCD (mouse): S643‑p, PKCD iso2 (mouse): S669‑p, PKCD (rat): S643‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
thrombin increase

S410-p - PKCI (mouse)
Modsite: LRPGDttstFCGtPN SwissProt Entrez-Gene
Orthologous residues
PKCI (human): S411‑p, PKCI (mouse): S410‑p, PKCI (rat): S411‑p

T538-p - PKCT (mouse)
Modsite: LGDAKtNtFCGTPDY SwissProt Entrez-Gene
Orthologous residues
PKCT (human): T538‑p, PKCT (mouse): T538‑p, PKCT (rat): T538‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
thrombin increase

T410-p - PKCZ (mouse)
Modsite: GPGDTTstFCGTPNY SwissProt Entrez-Gene
Orthologous residues
PKCZ (human): T410‑p, PKCZ iso2 (human): T227‑p, PKCZ iso3 (human): T306‑p, PKCZ (mouse): T410‑p, PKCZ (rat): T410‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse

S537-p - PLCB3 (mouse)
Modsite: TSLEPQKsLGEEsLS SwissProt Entrez-Gene
Orthologous residues
PLCB3 (human): S537‑p, PLCB3 iso2 (human): S470‑p, PLCB3 (mouse): S537‑p, PLCB3 (rat): S535‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  fibroblast-lung, HEK293T (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Cot (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Cot (mouse) transfection of wild-type enzyme, genetic knockout/knockin of upstream enzyme, siRNA inhibition of enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
thrombin increase
thrombin augment treatment-induced increase
KN-93 thrombin inhibit treatment-induced increase partial inhibition
siRNA decrease siRNA for Cot decreases phosphorylation
Downstream Regulation
Effect of modification (function):  phosphorylation
Effect of modification (process):  cell motility, induced, signaling pathway regulation
Comments:  FAK-Y397 phosphorylation, Tpl2-induced cell migration pathway

S744-p - PRKD1 (mouse)
Modsite: ARIIGEksFRRsVVG SwissProt Entrez-Gene
Orthologous residues
PRKD1 (human): S738‑p, PRKD1 (mouse): S744‑p, PRKD1 (rat): S744‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
thrombin increase

S748-p - PRKD1 (mouse)
Modsite: GEksFRRsVVGtPAy SwissProt Entrez-Gene
Orthologous residues
PRKD1 (human): S742‑p, PRKD1 (mouse): S748‑p, PRKD1 (rat): S748‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
thrombin increase

S916-p - PRKD1 (mouse)
Modsite: KALSERVsIL_____ SwissProt Entrez-Gene
Orthologous residues
PRKD1 (human): S910‑p, PRKD1 (mouse): S916‑p, PRKD1 (rat): S916‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HEK293T (epithelial), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
thrombin increase