Curated Information
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Home > Curated Information Page > PubMed Id: 21668450
Kelsall IR, et al. (2011) R3F, a novel membrane-associated glycogen targeting subunit of protein phosphatase 1 regulates glycogen synthase in astrocytoma cells in response to glucose and extracellular signals. J Neurochem 118, 596-610 21668450
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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S641-p - GYS1 (human)
Modsite: YRyPRPAsVPPsPsL SwissProt Entrez-Gene
Orthologous residues
GYS1 (human): S641‑p, GYS1 iso2 (human): S577‑p, GYS1 (mouse): S641‑p, GYS1 (rat): S641‑p, GYS1 (rabbit): S641‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  brain cancer, glioblastoma, glioma
Relevant cell lines - cell types - tissues:  293 (epithelial), U87MG (glial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
adenosine increase
norepinephrine increase
glucose_starvation increase
PPP1R3F (human) increase F39A mutant

S645-p - GYS1 (human)
Modsite: RPAsVPPsPsLsRHs SwissProt Entrez-Gene
Orthologous residues
GYS1 (human): S645‑p, GYS1 iso2 (human): S581‑p, GYS1 (mouse): S645‑p, GYS1 (rat): S645‑p, GYS1 (rabbit): S645‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  brain cancer, glioblastoma, glioma
Relevant cell lines - cell types - tissues:  293 (epithelial), U87MG (glial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
adenosine increase
norepinephrine increase
glucose_starvation increase
PPP1R3F (human) increase F39A mutant

S14-p - PPP1R3F (human)
Modsite: VEPPLrHsAPPsPAA SwissProt Entrez-Gene
Orthologous residues
PPP1R3F (human): S14‑p, PPP1R3F (mouse): P14‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Disease tissue studied:  brain cancer, glioblastoma, glioma
Relevant cell lines - cell types - tissues:  293 (epithelial), U87MG (glial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
adenosine decrease
glucose_starvation no change compared to control
CHIR99021 decrease

S18-p - PPP1R3F (human)
Modsite: LrHsAPPsPAAGEPR SwissProt Entrez-Gene
Orthologous residues
PPP1R3F (human): S18‑p, PPP1R3F (mouse): S18‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Disease tissue studied:  brain cancer, glioblastoma, glioma
Relevant cell lines - cell types - tissues:  293 (epithelial), U87MG (glial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
adenosine decrease
glucose_starvation no change compared to control
CHIR99021 decrease

S400-p - PPP1R3F (human)
Modsite: EEGDVPRssPPVAFT SwissProt Entrez-Gene
Orthologous residues
PPP1R3F (human): S400‑p, PPP1R3F (mouse): S397‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  brain cancer, glioblastoma, glioma
Relevant cell lines - cell types - tissues:  293 (epithelial), U87MG (glial)
Cellular systems studied:  cell lines
Species studied:  human

S401-p - PPP1R3F (human)
Modsite: EGDVPRssPPVAFTE SwissProt Entrez-Gene
Orthologous residues
PPP1R3F (human): S401‑p, PPP1R3F (mouse): N398‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  brain cancer, glioblastoma, glioma
Relevant cell lines - cell types - tissues:  293 (epithelial), U87MG (glial)
Cellular systems studied:  cell lines
Species studied:  human

S420-p - PPP1R3F (human)
Modsite: PAIRIPPssPLCGLG SwissProt Entrez-Gene
Orthologous residues
PPP1R3F (human): S420‑p, PPP1R3F (mouse): A417‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  brain cancer, glioblastoma, glioma
Relevant cell lines - cell types - tissues:  293 (epithelial), U87MG (glial)
Cellular systems studied:  cell lines
Species studied:  human

S421-p - PPP1R3F (human)
Modsite: AIRIPPssPLCGLGG SwissProt Entrez-Gene
Orthologous residues
PPP1R3F (human): S421‑p, PPP1R3F (mouse): T418‑p
Characterization
Methods used to characterize site in vivo mass spectrometry
Disease tissue studied:  brain cancer, glioblastoma, glioma
Relevant cell lines - cell types - tissues:  293 (epithelial), U87MG (glial)
Cellular systems studied:  cell lines
Species studied:  human

S15-p - PYGB (human)
Modsite: sEkRKQIsVRGLAGL SwissProt Entrez-Gene
Orthologous residues
PYGB (human): S15‑p, PYGB (mouse): S15‑p, PYGB (rat): S15‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  brain cancer, glioblastoma, glioma
Relevant cell lines - cell types - tissues:  brain, U87MG (glial)
Cellular systems studied:  cell lines, tissue
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
adenosine decrease
glucose_starvation decrease

S15-p - PYGB (mouse)
Modsite: sERQKQIsVRGIAGL SwissProt Entrez-Gene
Orthologous residues
PYGB (human): S15‑p, PYGB (mouse): S15‑p, PYGB (rat): S15‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  brain cancer, glioblastoma, glioma
Relevant cell lines - cell types - tissues:  brain, U87MG (glial)
Cellular systems studied:  cell lines, tissue
Species studied:  human, mouse