Curated Information
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Home > Curated Information Page > PubMed Id: 21726807
Sarkar S, et al. (2011) Complex inhibitory effects of nitric oxide on autophagy. Mol Cell 43, 19-32 21726807
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S70-p - Bcl-2 (human)
Modsite: RDPVARtsPLQtPAA SwissProt Entrez-Gene
Orthologous residues
Bcl‑2 (human): S70‑p, Bcl‑2 (mouse): S70‑p, Bcl‑2 (rat): S70‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cortical'-brain, HeLa (cervical), MEF (fibroblast)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE JNK1 (human) transfection of constitutively active enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nitric_oxide decrease
rapamycin no change compared to control
TSC2 (human) no change compared to control
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
beclin 1 (human) Disrupts molecular association, regulation autophagy, inhibited co-immunoprecipitation
Comments:  increases beclin1-hVps34 interaction

T183-p - JNK1 (human)
Modsite: AGtsFMMtPyVVtRY SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): T183‑p, JNK1 iso2 (human): T183‑p, JNK1 iso3 (human): T183‑p, JNK1 (mouse): T183‑p, JNK1 (rat): T183‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cortical'-brain, HeLa (cervical), MEF (fibroblast)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nitric_oxide decrease

Y185-p - JNK1 (human)
Modsite: tsFMMtPyVVtRYYR SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): Y185‑p, JNK1 iso2 (human): Y185‑p, JNK1 iso3 (human): Y185‑p, JNK1 (mouse): Y185‑p, JNK1 (rat): Y185‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cortical'-brain, HeLa (cervical), MEF (fibroblast)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nitric_oxide decrease

T412-p - p70S6K (human)
Modsite: NQVFLGFtyVAPsVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cortical'-brain, HeLa (cervical), MEF (fibroblast)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nitric_oxide increase

S235-p - S6 (human)
Modsite: IAKRRRLssLRAsts SwissProt Entrez-Gene
Orthologous residues
S6 (human): S235‑p, S6 (mouse): S235‑p, S6 (rat): S235‑p, S6 (fruit fly): A234‑p, S6 (cow): S235‑p
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cortical'-brain, HeLa (cervical), MEF (fibroblast)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nitric_oxide increase

S236-p - S6 (human)
Modsite: AKRRRLssLRAstsK SwissProt Entrez-Gene
Orthologous residues
S6 (human): S236‑p, S6 (mouse): S236‑p, S6 (rat): S236‑p, S6 (fruit fly): S235‑p, S6 (cow): S236‑p
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cortical'-brain, HeLa (cervical), MEF (fibroblast)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nitric_oxide increase

T183-p - AMPKA1 (mouse)
Modsite: sDGEFLRtsCGsPNY SwissProt Entrez-Gene
Orthologous residues
AMPKA1 (human): T183‑p, AMPKA1 iso2 (human): T198‑p, AMPKA1 (mouse): T183‑p, AMPKA1 (rat): T183‑p, AMPKA1 (fruit fly): T184‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cortical'-brain, HeLa (cervical), MEF (fibroblast)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nitric_oxide decrease

S176-p - IKKA (mouse)
Modsite: AKDVDQGsLCTsFVG SwissProt Entrez-Gene
Orthologous residues
IKKA (human): S176‑p, IKKA (mouse): S176‑p, IKKA (rat): S176‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cortical'-brain, HeLa (cervical), MEF (fibroblast)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nitric_oxide decrease

S180-p - IKKA (mouse)
Modsite: DQGsLCTsFVGTLQY SwissProt Entrez-Gene
Orthologous residues
IKKA (human): S180‑p, IKKA (mouse): S180‑p, IKKA (rat): S180‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cortical'-brain, HeLa (cervical), MEF (fibroblast)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nitric_oxide decrease

S177-p - IKKB (mouse)
Modsite: AKELDQGsLCTsFVG SwissProt Entrez-Gene
Orthologous residues
IKKB (human): S177‑p, IKKB (mouse): S177‑p, IKKB (rat): S177‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nitric_oxide decrease

S181-p - IKKB (mouse)
Modsite: DQGsLCTsFVGTLQy SwissProt Entrez-Gene
Orthologous residues
IKKB (human): S181‑p, IKKB (mouse): S181‑p, IKKB (rat): S181‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nitric_oxide decrease

T412-p - p70S6K (mouse)
Modsite: NQVFLGFtYVAPSVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cortical'-brain, HeLa (cervical), MEF (fibroblast)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nitric_oxide increase

S235-p - S6 (mouse)
Modsite: IAKRRRLssLRAsts SwissProt Entrez-Gene
Orthologous residues
S6 (human): S235‑p, S6 (mouse): S235‑p, S6 (rat): S235‑p, S6 (fruit fly): A234‑p, S6 (cow): S235‑p
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cortical'-brain, HeLa (cervical), MEF (fibroblast)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nitric_oxide increase

S236-p - S6 (mouse)
Modsite: AKRRRLssLRAstsK SwissProt Entrez-Gene
Orthologous residues
S6 (human): S236‑p, S6 (mouse): S236‑p, S6 (rat): S236‑p, S6 (fruit fly): S235‑p, S6 (cow): S236‑p
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'neuron, cortical'-brain, HeLa (cervical), MEF (fibroblast)
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
nitric_oxide increase

Y383-p - Cortactin (rat)
Modsite: RPPsSPIyEDtAPLK SwissProt Entrez-Gene
Orthologous residues
Cortactin (human): Y421‑p, Cortactin iso2 (human): Y384‑p, Cortactin iso3 (human): Y384‑p, Cortactin (mouse): Y421‑p, Cortactin (rat): Y383‑p