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Home > Curated Information Page > PubMed Id: 10559253
Ago T, Nunoi H, Ito T, Sumimoto H (1999) Mechanism for phosphorylation-induced activation of the phagocyte NADPH oxidase protein p47(phox). Triple replacement of serines 303, 304, and 328 with aspartates disrupts the SH3 domain-mediated intramolecular interaction in p47(phox), thereby activating the oxidase. J Biol Chem 274, 33644-53 10559253
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S303-p - p47phox (human)
Modsite: RGAPPRRssIRNAHs SwissProt Entrez-Gene
Orthologous residues
p47phox (human): S303‑p, p47phox (mouse): S304‑p, p47phox (rat): S304‑p
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
CYBA (human) Induces yeast two-hybrid, pull-down assay

S304-p - p47phox (human)
Modsite: GAPPRRssIRNAHsI SwissProt Entrez-Gene
Orthologous residues
p47phox (human): S304‑p, p47phox (mouse): T305‑p, p47phox (rat): T305‑p
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
CYBA (human) Induces yeast two-hybrid, pull-down assay

S315-p - p47phox (human)
Modsite: AHsIHQRsRKRLsQD SwissProt Entrez-Gene
Orthologous residues
p47phox (human): S315‑p, p47phox (mouse): S316‑p, p47phox (rat): S316‑p

S320-p - p47phox (human)
Modsite: QRsRKRLsQDAYRRN SwissProt Entrez-Gene
Orthologous residues
p47phox (human): S320‑p, p47phox (mouse): S321‑p, p47phox (rat): S321‑p

S328-p - p47phox (human)
Modsite: QDAYRRNsVRFLQQR SwissProt Entrez-Gene
Orthologous residues
p47phox (human): S328‑p, p47phox (mouse): S329‑p, p47phox (rat): S329‑p
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
CYBA (human) Induces yeast two-hybrid, pull-down assay

S345-p - p47phox (human)
Modsite: QARPGPQsPGsPLEE SwissProt Entrez-Gene
Orthologous residues
p47phox (human): S345‑p, p47phox (mouse): S346‑p, p47phox (rat): S346‑p

S348-p - p47phox (human)
Modsite: PGPQsPGsPLEEERQ SwissProt Entrez-Gene
Orthologous residues
p47phox (human): S348‑p, p47phox (mouse): G349‑p, p47phox (rat): S349‑p

S359-p - p47phox (human)
Modsite: EERQtQRsKPQPAVP SwissProt Entrez-Gene
Orthologous residues
p47phox (human): S359‑p, p47phox (mouse): V359‑p, p47phox (rat): A358‑p

S370-p - p47phox (human)
Modsite: PAVPPRPsADLILNR SwissProt Entrez-Gene
Orthologous residues
p47phox (human): S370‑p, p47phox (mouse): S370‑p, p47phox (rat): S369‑p

S379-p - p47phox (human)
Modsite: DLILNRCsEstKRKL SwissProt Entrez-Gene
Orthologous residues
p47phox (human): S379‑p, p47phox (mouse): T379‑p, p47phox (rat): T378‑p