Curated Information
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Home > Curated Information Page > PubMed Id: 18270262
Richardson C, et al. (2008) Activation of the thiazide-sensitive Na+-Cl- cotransporter by the WNK-regulated kinases SPAK and OSR1. J Cell Sci 121, 675-84 18270262
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T46-p - NCCT (human)
Modsite: SSHPSHLtHSStFCM SwissProt Entrez-Gene
Orthologous residues
NCCT (human): T46‑p, NCCT iso2 (human): T46‑p, NCCT iso3 (human): T46‑p, NCCT (mouse): T44‑p, NCCT (rat): T44‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE STLK3 (human)
KINASE OSR1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hypotonic_buffer increase

T55-p - NCCT (human)
Modsite: SStFCMRtFGYNtID SwissProt Entrez-Gene
Orthologous residues
NCCT (human): T55‑p, NCCT iso2 (human): T55‑p, NCCT iso3 (human): T55‑p, NCCT (mouse): T53‑p, NCCT (rat): T53‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE STLK3 (human)
KINASE OSR1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hypotonic_buffer increase

T60-p - NCCT (human)
Modsite: MRtFGYNtIDVVPtY SwissProt Entrez-Gene
Orthologous residues
NCCT (human): T60‑p, NCCT iso2 (human): T60‑p, NCCT iso3 (human): T60‑p, NCCT (mouse): T58‑p, NCCT (rat): T58‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE STLK3 (human)
KINASE OSR1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hypotonic_buffer increase
Downstream Regulation
Effect of modification (function):  activity, induced

S91-p - NCCT (human)
Modsite: PtLADLHsFLkQEGR SwissProt Entrez-Gene
Orthologous residues
NCCT (human): S91‑p, NCCT iso2 (human): S91‑p, NCCT iso3 (human): S91‑p, NCCT (mouse): S89‑p, NCCT (rat): S89‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, phospho-antibody
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hypotonic_buffer increase

T185-p - OSR1 (human)
Modsite: TRNKVRktFVGtPCW SwissProt Entrez-Gene
Orthologous residues
OSR1 (human): T185‑p, OSR1 (mouse): T185‑p, OSR1 (rat): T185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hypotonic_buffer increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S325-p - OSR1 (human)
Modsite: VRRVPGssGRLHKtE SwissProt Entrez-Gene
Orthologous residues
OSR1 (human): S325‑p, OSR1 (mouse): S325‑p, OSR1 (rat): S325‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hypotonic_buffer increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

T231-p - STLK3 (human)
Modsite: tRNKVRktFVGtPCW SwissProt Entrez-Gene
Orthologous residues
STLK3 (human): T231‑p, STLK3 (mouse): T243‑p, STLK3 (rat): T240‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hypotonic_buffer increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S371-p - STLK3 (human)
Modsite: VRRVPGssGHLHKTE SwissProt Entrez-Gene
Orthologous residues
STLK3 (human): S371‑p, STLK3 (mouse): S383‑p, STLK3 (rat): S380‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hypotonic_buffer increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

S382-p - WNK1 (human)
Modsite: kRAsFAKsVIGTPEF SwissProt Entrez-Gene
Orthologous residues
WNK1 (human): S382‑p, WNK1 iso2 (human): S382‑p, WNK1 iso4 (human): , WNK1 iso5 (human): S382‑p, WNK1 iso7 (human): S382‑p, WNK1 (mouse): S382‑p, WNK1 iso3 (mouse): S382‑p, WNK1 (rat): S382‑p, WNK1 (cow): S387‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hypotonic_buffer increase
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced

T44-p - NCCT (mouse)
Modsite: ssQPsHLtHGstLyM SwissProt Entrez-Gene
Orthologous residues
NCCT (human): T46‑p, NCCT iso2 (human): T46‑p, NCCT iso3 (human): T46‑p, NCCT (mouse): T44‑p, NCCT (rat): T44‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  mpkCCD (renal)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hypotonic_buffer increase

T53-p - NCCT (mouse)
Modsite: GstLyMRtFGYNtID SwissProt Entrez-Gene
Orthologous residues
NCCT (human): T55‑p, NCCT iso2 (human): T55‑p, NCCT iso3 (human): T55‑p, NCCT (mouse): T53‑p, NCCT (rat): T53‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  mpkCCD (renal)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hypotonic_buffer increase

T58-p - NCCT (mouse)
Modsite: MRtFGYNtIDVVPAY SwissProt Entrez-Gene
Orthologous residues
NCCT (human): T60‑p, NCCT iso2 (human): T60‑p, NCCT iso3 (human): T60‑p, NCCT (mouse): T58‑p, NCCT (rat): T58‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Relevant cell lines - cell types - tissues:  mpkCCD (renal)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hypotonic_buffer increase

T185-p - OSR1 (mouse)
Modsite: TRNKVRKtFVGtPCW SwissProt Entrez-Gene
Orthologous residues
OSR1 (human): T185‑p, OSR1 (mouse): T185‑p, OSR1 (rat): T185‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hypotonic_buffer increase

S325-p - OSR1 (mouse)
Modsite: VRRVPGssGRLHKtE SwissProt Entrez-Gene
Orthologous residues
OSR1 (human): S325‑p, OSR1 (mouse): S325‑p, OSR1 (rat): S325‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hypotonic_buffer increase

S382-p - WNK1 (mouse)
Modsite: KRAsFAKsVIGTPEF SwissProt Entrez-Gene
Orthologous residues
WNK1 (human): S382‑p, WNK1 iso2 (human): S382‑p, WNK1 iso4 (human): , WNK1 iso5 (human): S382‑p, WNK1 iso7 (human): S382‑p, WNK1 (mouse): S382‑p, WNK1 iso3 (mouse): S382‑p, WNK1 (rat): S382‑p, WNK1 (cow): S387‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hypotonic_buffer increase

T240-p - STLK3 (rat)
Modsite: TRNKVRKtFVGTPCW SwissProt Entrez-Gene
Orthologous residues
STLK3 (human): T231‑p, STLK3 (mouse): T243‑p, STLK3 (rat): T240‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hypotonic_buffer increase

S380-p - STLK3 (rat)
Modsite: VRRVPGSsGHLHKTE SwissProt Entrez-Gene
Orthologous residues
STLK3 (human): S371‑p, STLK3 (mouse): S383‑p, STLK3 (rat): S380‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
hypotonic_buffer increase