Mollah S, et al. (2007) Targeted mass spectrometric strategy for global mapping of ubiquitination on proteins. Rapid Commun Mass Spectrom 21, 3357-64 17883243

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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K115-ub - RIPK1 (human) ▲

Modsite: MSTPLSVkGRIILEI SwissProt Entrez-Gene Orthologous residues RIPK1 (human): K115‑ub, RIPK1 iso2 (human): ‑, RIPK1 (mouse): K115‑ub, RIPK1 (rat): K115‑ub Characterization Methods used to characterize site in vivo:  electrophoretic mobility shift, mass spectrometry, modification-specific antibody, multiple reaction monitoring (MRM), western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme DEUBIQUITINASE USP18 (human)

K571-ub - RIPK1 (human) ▲

Modsite: FkEEPAAkYQAIFDN SwissProt Entrez-Gene Orthologous residues RIPK1 (human): K571‑ub, RIPK1 iso2 (human): K525‑ub, RIPK1 (mouse): R556‑ub, RIPK1 (rat): R558‑ub Characterization Methods used to characterize site in vivo:  electrophoretic mobility shift, mass spectrometry, modification-specific antibody, multiple reaction monitoring (MRM), western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme DEUBIQUITINASE USP18 (human)

K604-ub - RIPK1 (human) ▲

Modsite: HWKNCARkLGFTQsQ SwissProt Entrez-Gene Orthologous residues RIPK1 (human): K604‑ub, RIPK1 iso2 (human): K558‑ub, RIPK1 (mouse): K589‑ub, RIPK1 (rat): K591‑ub Characterization Methods used to characterize site in vivo:  electrophoretic mobility shift, mass spectrometry, modification-specific antibody, multiple reaction monitoring (MRM), western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme DEUBIQUITINASE USP18 (human)

K627-ub - RIPK1 (human) ▲

Modsite: ERDGLKEkVYQMLQk SwissProt Entrez-Gene Orthologous residues RIPK1 (human): K627‑ub, RIPK1 iso2 (human): K581‑ub, RIPK1 (mouse): K612‑ub, RIPK1 (rat): K614‑ub Characterization Methods used to characterize site in vivo:  electrophoretic mobility shift, mass spectrometry, modification-specific antibody, multiple reaction monitoring (MRM), western blotting Relevant cell lines - cell types - tissues:  293 (epithelial), HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme DEUBIQUITINASE USP18 (human)