Chen HZ, et al. (2012) Prolyl isomerase Pin1 stabilizes and activates orphan nuclear receptor TR3 to promote mitogenesis. Oncogene 31, 2876-87 22002310

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S95-p - Nur77 (human) ▲

Modsite: TSSSSATsPASASFk SwissProt Entrez-Gene Orthologous residues Nur77 (human): S95‑p, Nur77 iso2 (human): S108‑p, Nur77 (mouse): S97‑p, Nur77 (rat): S94‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes EGF increase SP600125 EGF inhibit treatment-induced increase Downstream Regulation Effect of modification (function):  molecular association, regulation, protein stabilization Effect of modification (process):  cell cycle regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays PIN1 (human) WW Induces molecular association, regulation pull-down assay, co-immunoprecipitation Comments:  interaction with Pin1 enhances Nur77 ability to interact with p300, required for cell proliferation

S140-p - Nur77 (human) ▲

Modsite: GSPCSAPsPStPSFQ SwissProt Entrez-Gene Orthologous residues Nur77 (human): S140‑p, Nur77 iso2 (human): S153‑p, Nur77 (mouse): S142‑p, Nur77 (rat): S139‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  cell cycle regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays PIN1 (human) WW Induces molecular association, regulation pull-down assay, co-immunoprecipitation Comments:  interaction with Pin1 enhances Nur77 ability to interact with p300, required for cell proliferation

S431-p - Nur77 (human) ▲

Modsite: IPGFAELsPADQDLL SwissProt Entrez-Gene Orthologous residues Nur77 (human): S431‑p, Nur77 iso2 (human): S444‑p, Nur77 (mouse): C434‑p, Nur77 (rat): S430‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE ERK2 (human) siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes EGF increase PD98059 EGF inhibit treatment-induced increase siRNA EGF inhibit treatment-induced increase ERK2 targeting siRNA Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  cell cycle regulation, transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays PIN1 (human) WW Induces molecular association, regulation pull-down assay, co-immunoprecipitation Comments:  interaction with Pin1 enhances Nur77 ability to interact with p300, required for cell proliferation