Curated Information
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Home > Curated Information Page > PubMed Id: 21617041
Wild P, et al. (2011) Phosphorylation of the autophagy receptor optineurin restricts Salmonella growth. Science 333, 228-33 21617041
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S177-p - optineurin (human)
Modsite: ssGssEDsFVEIRMA SwissProt Entrez-Gene
Orthologous residues
optineurin (human): S177‑p, optineurin iso2 (human): S177‑p, optineurin (mouse): S187‑p, optineurin (rat): S188‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, microscopy-colocalization with upstream kinase
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE TBK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase
BX795 LPS inhibit treatment-induced increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  autophagy, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SPTLC3 (human) Induces co-immunoprecipitation
Comments:  autophagic clearance of cytosolic Salmonella

S388-p - IRF3 (mouse)
Modsite: KTVDLHIsNSQPISL SwissProt Entrez-Gene
Orthologous residues
IRF3 (human): S396‑p, IRF3 (mouse): S388‑p, IRF3 (rat): S390‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase
BX795 LPS inhibit treatment-induced increase

S187-p - optineurin (mouse)
Modsite: SGGSsEDsFVEIRMT SwissProt Entrez-Gene
Orthologous residues
optineurin (human): S177‑p, optineurin iso2 (human): S177‑p, optineurin (mouse): S187‑p, optineurin (rat): S188‑p
Characterization
Methods used to characterize site in vivo mass spectrometry, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE TBK1 (human) pharmacological inhibitor of upstream enzyme, phospho-antibody, modification site within consensus motif, microscopy-colocalization