Curated Information
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Home > Curated Information Page > PubMed Id: 21444715
Vigneron S, et al. (2011) Characterization of the mechanisms controlling Greatwall activity. Mol Cell Biol 31, 2262-75 21444715
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T75-p - MASTL (human)
Modsite: DMINkNMtHQVQAER SwissProt Entrez-Gene
Orthologous residues
MASTL (human): T75‑p, MASTL iso2 (human): T75‑p, MASTL (mouse): T74‑p, MASTL (rat): T74‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (frog)

S512-p - MASTL (human)
Modsite: NkENIVNsFtDkQQt SwissProt Entrez-Gene
Orthologous residues
MASTL (human): S512‑p, MASTL iso2 (human): S512‑p, MASTL (mouse): Y502‑p, MASTL (rat): Y503‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (frog)

S552-p - MASTL (human)
Modsite: KRDYLsssFLCsDDD SwissProt Entrez-Gene
Orthologous residues
MASTL (human): S552‑p, MASTL iso2 (human): S552‑p, MASTL (mouse): N541‑p, MASTL (rat): N542‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (frog)

S597-p - MASTL (human)
Modsite: SDRSIKEssFEESNI SwissProt Entrez-Gene
Orthologous residues
MASTL (human): S597‑p, MASTL iso2 (human): S597‑p, MASTL (mouse): Y586‑p, MASTL (rat): Y587‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (frog)

S598-p - MASTL (human)
Modsite: DRSIKEssFEESNIE SwissProt Entrez-Gene
Orthologous residues
MASTL (human): S598‑p, MASTL iso2 (human): S598‑p, MASTL (mouse): S587‑p, MASTL (rat): S588‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (frog)

T860-p - MASTL (human)
Modsite: QPDDETDtSYFEARN SwissProt Entrez-Gene
Orthologous residues
MASTL (human): T860‑p, MASTL iso2 (human): T821‑p, MASTL (mouse): T846‑p, MASTL (rat): T847‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (frog)
Downstream Regulation
Effect of modification (function):  protein conformation

T873-p - MASTL (human)
Modsite: RNtAQHLtVsGFsL_ SwissProt Entrez-Gene
Orthologous residues
MASTL (human): T873‑p, MASTL iso2 (human): T834‑p, MASTL (mouse): T859‑p, MASTL (rat): T860‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro)
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PLK1 (frog)

S875-p - MASTL (human)
Modsite: tAQHLtVsGFsL___ SwissProt Entrez-Gene
Orthologous residues
MASTL (human): S875‑p, MASTL iso2 (human): S836‑p, MASTL (mouse): S861‑p, MASTL (rat): S862‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mass spectrometry (in vitro), mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  oocyte
Cellular systems studied:  primary cells
Species studied:  frog
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CDK1 (human)
KINASE PLK1 (frog)
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, phosphorylation, protein conformation
Effect of modification (process):  cell cycle regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
RSK2 (human) PIF Induces in vitro
Comments:  phosphorylation induces intramolecular interaction, which inhibits dephosphorylation at the same phospho site.