Vasilescu J, et al. (2007) The proteomic reactor facilitates the analysis of affinity-purified proteins by mass spectrometry: application for identifying ubiquitinated proteins in human cells. J Proteome Res 6, 298-305 17203973

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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K334-ub - 5-HT(1A) (human) ▲

Modsite: RNAEAKRkMALARER SwissProt Entrez-Gene Orthologous residues 5‑HT(1A) (human): K334‑ub, 5‑HT(1A) (mouse): K334‑ub, 5‑HT(1A) (rat): K334‑ub Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary) [VCP (human), transfection, VCP siRNA construct was used to transfect cells] Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes siRNA increase VCP siRNA VCP (human) decrease

K402-ub - ASH1L (human) ▲

Modsite: SAMGLVNkDIGkKLM SwissProt Entrez-Gene Orthologous residues ASH1L (human): K402‑ub, ASH1L iso2 (human): K402‑ub, ASH1L (mouse): K400‑ub, ASH1L (rat): K400‑ub Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary) [VCP (human), transfection, VCP siRNA construct was used to transfect cells] Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes siRNA increase VCP siRNA VCP (human) decrease

K406-ub - ASH1L (human) ▲

Modsite: LVNkDIGkKLMSCPL SwissProt Entrez-Gene Orthologous residues ASH1L (human): K406‑ub, ASH1L iso2 (human): K406‑ub, ASH1L (mouse): K404‑ub, ASH1L (rat): K404‑ub Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary) [VCP (human), transfection, VCP siRNA construct was used to transfect cells] Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes siRNA increase VCP siRNA VCP (human) decrease

K46-ub - ATAD1 (human) ▲

Modsite: DAIDPTRkQKVEAQk SwissProt Entrez-Gene Orthologous residues ATAD1 (human): K46‑ub, ATAD1 (mouse): K46‑ub, ATAD1 (rat): K46‑ub Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary) [VCP (human), transfection, VCP siRNA construct was used to transfect cells] Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes siRNA increase VCP siRNA VCP (human) decrease

K96-ub - ATRIP (human) ▲

Modsite: RLLDGMSkNPSGKNR SwissProt Entrez-Gene Orthologous residues ATRIP (human): K96‑ub, ATRIP iso2 (human): K96‑ub, ATRIP (mouse): N96‑ub, ATRIP (rat): N96‑ub Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary) [VCP (human), transfection, VCP siRNA construct was used to transfect cells] Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes siRNA increase VCP siRNA VCP (human) decrease

K816-ub - BAT2L iso5 (human) ▲

Modsite: LPEAsSKkAEKEAKL SwissProt Entrez-Gene Orthologous residues BAT2L (human): K1510‑ub, BAT2L iso5 (human): K816‑ub, BAT2L (mouse): K827‑ub, BAT2L iso3 (mouse): K1514‑ub, BAT2L (rat): K1359‑ub Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary), NCI-H1299 (pulmonary) [VCP (human), transfection, VCP siRNA construct was used to transfect cells] Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes siRNA increase VCP siRNA VCP (human) decrease

K535-ub - CBFA2T3 (human) ▲

Modsite: ERALAEAkRQAsEDA SwissProt Entrez-Gene Orthologous residues CBFA2T3 (human): K535‑ub, CBFA2T3 (mouse): K512‑ub, CBFA2T3 (rat): K511‑ub Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary) [VCP (human), transfection, VCP siRNA construct was used to transfect cells] Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes siRNA increase VCP siRNA VCP (human) decrease

K295-ub - CCDC138 (human) ▲

Modsite: EASEENRkIDIQAkR SwissProt Entrez-Gene Orthologous residues CCDC138 (human): K295‑ub, CCDC138 iso2 (human): K295‑ub, CCDC138 (mouse): K311‑ub, CCDC138 (rat): K312‑ub Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary), NCI-H1299 (pulmonary) [VCP (human), transfection, VCP siRNA construct was used to transfect cells] Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes siRNA increase VCP siRNA VCP (human) decrease

K225-ub - FAM76B iso2 (human) ▲

Modsite: KKPKLESkPSNGDRS SwissProt Entrez-Gene Orthologous residues FAM76B (human): K225‑ub, FAM76B iso2 (human): K225‑ub, FAM76B (mouse): K225‑ub, FAM76B (rat): K225‑ub Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary) [VCP (human), transfection, VCP siRNA construct was used to transfect cells] Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes siRNA increase VCP siRNA VCP (human) decrease

K113-ub - Fos (human) ▲

Modsite: YSRAGVVktMtGGRA SwissProt Entrez-Gene Orthologous residues Fos (human): K113‑ub, Fos (mouse): K113‑ub, Fos (rat): K113‑ub Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary) [VCP (human), transfection, VCP siRNA construct was used to transfect cells] Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes siRNA increase VCP siRNA VCP (human) decrease

K102-ub - GUF1 (human) ▲
Modsite: IDKTKNNkQVLDKLQ SwissProt Entrez-Gene Orthologous residues GUF1 (human): K102‑ub, GUF1 (mouse): K84‑ub Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary) Cellular systems studied:  cell lines Species studied:  human

K191-ub - HOXB7 (human) ▲
Modsite: WFQNRRMkWkKENKT SwissProt Entrez-Gene Orthologous residues HOXB7 (human): K191‑ub, HOXB7 (mouse): K191‑ub, HOXB7 (rat): K191‑ub Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary) Cellular systems studied:  cell lines Species studied:  human

K193-ub - HOXB7 (human) ▲
Modsite: QNRRMkWkKENKTAG SwissProt Entrez-Gene Orthologous residues HOXB7 (human): K193‑ub, HOXB7 (mouse): K193‑ub, HOXB7 (rat): K193‑ub Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary) Cellular systems studied:  cell lines Species studied:  human

K483-ub - HUNK (human) ▲

Modsite: QNTKALLkDRKASKS SwissProt Entrez-Gene Orthologous residues HUNK (human): K483‑ub, HUNK (mouse): K483‑ub, HUNK (rat): K483‑ub Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary) [VCP (human), transfection, VCP siRNA construct was used to transfect cells] Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes siRNA increase VCP siRNA VCP (human) decrease

K275-ub - KIF14 (human) ▲
Modsite: NKFGsLEkRtPTKCT SwissProt Entrez-Gene Orthologous residues KIF14 (human): K275‑ub, KIF14 (mouse): T260‑ub Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary) Cellular systems studied:  cell lines Species studied:  human

K685-ub - KIF16B iso5 (human) ▲

Modsite: KFEEERLkDLVQQKD SwissProt Entrez-Gene Orthologous residues KIF16B (human): K841‑ub, KIF16B iso2 (human): K841‑ub, KIF16B iso5 (human): K685‑ub, KIF16B (mouse): K841‑ub, KIF16B iso2 (mouse): K438‑ub Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary), NCI-H1299 (pulmonary) [VCP (human), transfection, VCP siRNA construct was used to transfect cells] Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes siRNA increase VCP siRNA VCP (human) decrease

K216-ub - MLL (human) ▲

Modsite: KIKKKDSkSIEkkRG SwissProt Entrez-Gene Orthologous residues MLL (human): K216‑ub, MLL iso2 (human): K216‑ub, MLL iso3 (human): K216‑ub, MLL (mouse): K214‑ub, MLL iso5 (mouse): K247‑ub, MLL (rat): ‑ Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary) Cellular systems studied:  cell lines Species studied:  human

K220-ub - MLL (human) ▲

Modsite: KDSkSIEkkRGRPPT SwissProt Entrez-Gene Orthologous residues MLL (human): K220‑ub, MLL iso2 (human): K220‑ub, MLL iso3 (human): K220‑ub, MLL (mouse): K218‑ub, MLL iso5 (mouse): K251‑ub, MLL (rat): ‑ Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary) Cellular systems studied:  cell lines Species studied:  human

K221-ub - MLL (human) ▲

Modsite: DSkSIEkkRGRPPTF SwissProt Entrez-Gene Orthologous residues MLL (human): K221‑ub, MLL iso2 (human): K221‑ub, MLL iso3 (human): K221‑ub, MLL (mouse): K219‑ub, MLL iso5 (mouse): K252‑ub, MLL (rat): ‑ Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary) Cellular systems studied:  cell lines Species studied:  human

K222-ub - TARS (human) ▲

Modsite: AFERLEVkkETLLAM SwissProt Entrez-Gene Orthologous residues TARS (human): K222‑ub, TARS iso2 (human): K255‑ub, TARS (mouse): K221‑ub, TARS (rat): K194‑ub Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary) [VCP (human), transfection, VCP siRNA construct was used to transfect cells] Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes siRNA increase VCP siRNA VCP (human) decrease

K267-ub - TRAF3IP3 (human) ▲
Modsite: ysPWGMKkVLLEMED SwissProt Entrez-Gene Orthologous residues TRAF3IP3 (human): K267‑ub, TRAF3IP3 (mouse): K268‑ub Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary) Cellular systems studied:  cell lines Species studied:  human

K48-ub - ubiquitin (human) ▲

Modsite: QRLIFAGkQLEDGRT SwissProt Orthologous residues ubiquitin (human): K48‑ub, ubiquitin (mouse): K48‑ub, ubiquitin (rat): K48‑ub, ubiquitin (cow): K48‑ub Characterization Methods used to characterize site in vivo:  mass spectrometry Relevant cell lines - cell types - tissues:  NCI-H1299 (pulmonary), NCI-H1299 (pulmonary) [VCP (human), transfection, VCP siRNA construct was used to transfect cells] Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes siRNA increase VCP siRNA VCP (human) decrease