Oeckinghaus A, et al. (2007) Malt1 ubiquitination triggers NF-kappaB signaling upon T-cell activation. EMBO J 26, 4634-45 17948050

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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K644-ub - MALT1 (human) ▲

Modsite: LDLDIDPkDANkGTP SwissProt Entrez-Gene Orthologous residues MALT1 (human): K644‑ub, MALT1 iso2 (human): K633‑ub, MALT1 (mouse): K652‑ub, MALT1 (rat): K652‑ub Characterization Methods used to characterize site in vivo:  mutation of modification site Disease tissue studied:  leukemia, T cell leukemia Relevant cell lines - cell types - tissues:  HEK293T (epithelial), Jurkat (T lymphocyte), T lymphocyte-lymph node, T lymphocyte-spleen Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme UBIQUITIN LIGASE TRAF6 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes UBIQUITIN LIGASE TRAF6 (human) siRNA inhibition of enzyme, transfection of inactive enzyme, co-immunoprecipitation, transfection of wild-type enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation, protein degradation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays IKKA (human) Induces co-immunoprecipitation Comments:  activation of NF-kB pathway and IL-2 induction

K648-ub - MALT1 (human) ▲

Modsite: IDPkDANkGTPEETG SwissProt Entrez-Gene Orthologous residues MALT1 (human): K648‑ub, MALT1 iso2 (human): K637‑ub, MALT1 (mouse): K656‑ub, MALT1 (rat): K656‑ub Characterization Methods used to characterize site in vivo:  mutation of modification site Disease tissue studied:  leukemia, T cell leukemia Relevant cell lines - cell types - tissues:  HEK293T (epithelial), Jurkat (T lymphocyte), T lymphocyte-lymph node, T lymphocyte-spleen Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme UBIQUITIN LIGASE TRAF6 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes UBIQUITIN LIGASE TRAF6 (human) siRNA inhibition of enzyme, transfection of inactive enzyme, co-immunoprecipitation, transfection of wild-type enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation, protein degradation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays IKKA (human) Induces co-immunoprecipitation Comments:  activation of NF-kB pathway and IL-2 induction

K661-ub - MALT1 (human) ▲

Modsite: TGsYLVskDLPkHCL SwissProt Entrez-Gene Orthologous residues MALT1 (human): K661‑ub, MALT1 iso2 (human): K650‑ub, MALT1 (mouse): K669‑ub, MALT1 (rat): K669‑ub Characterization Methods used to characterize site in vivo:  mutation of modification site Disease tissue studied:  leukemia, T cell leukemia Relevant cell lines - cell types - tissues:  HEK293T (epithelial), Jurkat (T lymphocyte), T lymphocyte-lymph node, T lymphocyte-spleen Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme UBIQUITIN LIGASE TRAF6 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes UBIQUITIN LIGASE TRAF6 (human) siRNA inhibition of enzyme, transfection of inactive enzyme, co-immunoprecipitation, transfection of wild-type enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation, protein degradation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays IKKA (human) Induces co-immunoprecipitation Comments:  activation of NF-kB pathway and IL-2 induction

K665-ub - MALT1 (human) ▲

Modsite: LVskDLPkHCLYTRL SwissProt Entrez-Gene Orthologous residues MALT1 (human): K665‑ub, MALT1 iso2 (human): K654‑ub, MALT1 (mouse): K673‑ub, MALT1 (rat): K673‑ub Characterization Methods used to characterize site in vivo:  mutation of modification site Disease tissue studied:  leukemia, T cell leukemia Relevant cell lines - cell types - tissues:  HEK293T (epithelial), Jurkat (T lymphocyte), T lymphocyte-lymph node, T lymphocyte-spleen Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme UBIQUITIN LIGASE TRAF6 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes UBIQUITIN LIGASE TRAF6 (human) siRNA inhibition of enzyme, transfection of inactive enzyme, co-immunoprecipitation, transfection of wild-type enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation, protein degradation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays IKKA (human) Induces co-immunoprecipitation Comments:  activation of NF-kB pathway and IL-2 induction

K677-ub - MALT1 (human) ▲

Modsite: TRLSsLQkLkEHLVF SwissProt Entrez-Gene Orthologous residues MALT1 (human): K677‑ub, MALT1 iso2 (human): K666‑ub, MALT1 (mouse): K685‑ub, MALT1 (rat): K685‑ub Characterization Methods used to characterize site in vivo:  mutation of modification site Disease tissue studied:  leukemia, T cell leukemia Relevant cell lines - cell types - tissues:  HEK293T (epithelial), Jurkat (T lymphocyte), T lymphocyte-lymph node, T lymphocyte-spleen Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme UBIQUITIN LIGASE TRAF6 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes UBIQUITIN LIGASE TRAF6 (human) siRNA inhibition of enzyme, transfection of inactive enzyme, co-immunoprecipitation, transfection of wild-type enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation, protein degradation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays IKKA (human) Induces co-immunoprecipitation Comments:  activation of NF-kB pathway and IL-2 induction

K679-ub - MALT1 (human) ▲

Modsite: LSsLQkLkEHLVFTV SwissProt Entrez-Gene Orthologous residues MALT1 (human): K679‑ub, MALT1 iso2 (human): K668‑ub, MALT1 (mouse): K687‑ub, MALT1 (rat): K687‑ub Characterization Methods used to characterize site in vivo:  mutation of modification site Disease tissue studied:  leukemia, T cell leukemia Relevant cell lines - cell types - tissues:  HEK293T (epithelial), Jurkat (T lymphocyte), T lymphocyte-lymph node, T lymphocyte-spleen Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme UBIQUITIN LIGASE TRAF6 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes UBIQUITIN LIGASE TRAF6 (human) siRNA inhibition of enzyme, transfection of inactive enzyme, co-immunoprecipitation, transfection of wild-type enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation, protein degradation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays IKKA (human) Induces co-immunoprecipitation Comments:  activation of NF-kB pathway and IL-2 induction

K702-ub - MALT1 (human) ▲

Modsite: LEDTVEDkQEVNVGk SwissProt Entrez-Gene Orthologous residues MALT1 (human): K702‑ub, MALT1 iso2 (human): K691‑ub, MALT1 (mouse): K710‑ub, MALT1 (rat): K710‑ub Characterization Methods used to characterize site in vivo:  mutation of modification site Disease tissue studied:  leukemia, T cell leukemia Relevant cell lines - cell types - tissues:  HEK293T (epithelial), Jurkat (T lymphocyte), T lymphocyte-lymph node, T lymphocyte-spleen Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme UBIQUITIN LIGASE TRAF6 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes UBIQUITIN LIGASE TRAF6 (human) siRNA inhibition of enzyme, transfection of inactive enzyme, co-immunoprecipitation, transfection of wild-type enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation, protein degradation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays IKKA (human) Induces signaling pathway regulation co-immunoprecipitation Comments:  activation of NF-kB pathway and IL-2 induction

K709-ub - MALT1 (human) ▲

Modsite: kQEVNVGkPLIAkLD SwissProt Entrez-Gene Orthologous residues MALT1 (human): K709‑ub, MALT1 iso2 (human): K698‑ub, MALT1 (mouse): K717‑ub, MALT1 (rat): K717‑ub Characterization Methods used to characterize site in vivo:  mutation of modification site Disease tissue studied:  leukemia, T cell leukemia Relevant cell lines - cell types - tissues:  HEK293T (epithelial), Jurkat (T lymphocyte), T lymphocyte-lymph node, T lymphocyte-spleen Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme UBIQUITIN LIGASE TRAF6 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes UBIQUITIN LIGASE TRAF6 (human) siRNA inhibition of enzyme, transfection of inactive enzyme, co-immunoprecipitation, transfection of wild-type enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation, protein degradation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays IKKA (human) Induces co-immunoprecipitation Comments:  activation of NF-kB pathway and IL-2 induction

K714-ub - MALT1 (human) ▲

Modsite: VGkPLIAkLDMHRGL SwissProt Entrez-Gene Orthologous residues MALT1 (human): K714‑ub, MALT1 iso2 (human): K703‑ub, MALT1 (mouse): K722‑ub, MALT1 (rat): K722‑ub Characterization Methods used to characterize site in vivo:  mutation of modification site Disease tissue studied:  leukemia, T cell leukemia Relevant cell lines - cell types - tissues:  HEK293T (epithelial), Jurkat (T lymphocyte), T lymphocyte-lymph node, T lymphocyte-spleen Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme UBIQUITIN LIGASE TRAF6 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes UBIQUITIN LIGASE TRAF6 (human) siRNA inhibition of enzyme, transfection of inactive enzyme, co-immunoprecipitation, transfection of wild-type enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation, protein degradation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays IKKA (human) Induces co-immunoprecipitation Comments:  activation of NF-kB pathway and IL-2 induction

K724-ub - MALT1 (human) ▲

Modsite: MHRGLGRkTCFQTCL SwissProt Entrez-Gene Orthologous residues MALT1 (human): K724‑ub, MALT1 iso2 (human): K713‑ub, MALT1 (mouse): K732‑ub, MALT1 (rat): K732‑ub Characterization Methods used to characterize site in vivo:  mutation of modification site Disease tissue studied:  leukemia, T cell leukemia Relevant cell lines - cell types - tissues:  HEK293T (epithelial), Jurkat (T lymphocyte), T lymphocyte-lymph node, T lymphocyte-spleen Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme UBIQUITIN LIGASE TRAF6 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes UBIQUITIN LIGASE TRAF6 (human) siRNA inhibition of enzyme, transfection of inactive enzyme, co-immunoprecipitation, transfection of wild-type enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation, protein degradation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays IKKA (human) Induces co-immunoprecipitation Comments:  activation of NF-kB pathway and IL-2 induction

K824-ub - MALT1 (human) ▲

Modsite: DRLRISEk_______ SwissProt Entrez-Gene Orthologous residues MALT1 (human): K824‑ub, MALT1 iso2 (human): K813‑ub, MALT1 (mouse): N832‑ub, MALT1 (rat): N832‑ub Characterization Methods used to characterize site in vivo:  mutation of modification site Disease tissue studied:  leukemia, T cell leukemia Relevant cell lines - cell types - tissues:  HEK293T (epithelial), Jurkat (T lymphocyte), T lymphocyte-lymph node, T lymphocyte-spleen Cellular systems studied:  cell lines Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme UBIQUITIN LIGASE TRAF6 (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes UBIQUITIN LIGASE TRAF6 (human) siRNA inhibition of enzyme, transfection of inactive enzyme, co-immunoprecipitation, transfection of wild-type enzyme Downstream Regulation Effect of modification (function):  molecular association, regulation, protein degradation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays IKKA (human) Induces co-immunoprecipitation Comments:  activation of NF-kB pathway and IL-2 induction