Curated Information
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Home > Curated Information Page > PubMed Id: 21474066
Kennedy AL, et al. (2011) Activation of the PIK3CA/AKT pathway suppresses senescence induced by an activated RAS oncogene to promote tumorigenesis. Mol Cell 42, 36-49 21474066
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S473-p - Akt1 (human)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Disease tissue studied:  pancreatic cancer
Relevant cell lines - cell types - tissues:  pancreatic
Cellular systems studied:  tissue
Species studied:  human
Associated Diseases
Diseases Alterations Comments
pancreatic cancer increased phosphorylation associated with poor overall survival

S9-p - GSK3B (human)
Modsite: SGRPRttsFAEsCkP SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S9‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  pancreatic cancer
Relevant cell lines - cell types - tissues:  IMR-90 (fibroblast), pancreatic
Cellular systems studied:  cell lines, tissue
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Akt1 (human) increase constitutively active Akt1
HRas (human) Akt1 (human) no effect upon treatment-induced increase activated HRas
HRas (human) no change compared to control activated HRas
rapamycin no change compared to control
Associated Diseases
Diseases Alterations Comments
pancreatic cancer increased phosphorylation associated with poor overall survival

S2448-p - mTOR (human)
Modsite: RsRtRtDsysAGQsV SwissProt Entrez-Gene
Orthologous residues
mTOR (human): S2448‑p, mTOR (mouse): S2448‑p, mTOR (rat): S2448‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Disease tissue studied:  pancreatic cancer
Relevant cell lines - cell types - tissues:  pancreatic
Cellular systems studied:  tissue
Species studied:  human
Associated Diseases
Diseases Alterations Comments
pancreatic cancer increased phosphorylation associated with poor overall survival

T412-p - p70S6K (human)
Modsite: NQVFLGFtyVAPsVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  IMR-90 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Akt1 (human) increase constitutively active Akt1
HRas (human) Akt1 (human) no effect upon treatment-induced increase activated HRas
HRas (human) no change compared to control activated HRas
rapamycin no change compared to control

S780-p - Rb (human)
Modsite: strPPtLsPIPHIPr SwissProt Entrez-Gene
Orthologous residues
Rb (human): S780‑p, Rb (mouse): S773‑p, Rb (rat): S772‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  IMR-90 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
HRas (human) increase activated HRas
Akt1 (human) decrease activated Akt1

S807-p - Rb (human)
Modsite: PGGNIyIsPLksPyk SwissProt Entrez-Gene
Orthologous residues
Rb (human): S807‑p, Rb (mouse): S800‑p, Rb (rat): S799‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  IMR-90 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
HRas (human) increase activated HRas
Akt1 (human) decrease activated Akt1

S811-p - Rb (human)
Modsite: IyIsPLksPykIsEG SwissProt Entrez-Gene
Orthologous residues
Rb (human): S811‑p, Rb (mouse): S804‑p, Rb (rat): S803‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  IMR-90 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
HRas (human) increase activated HRas
Akt1 (human) decrease activated Akt1

S473-p - Akt1 (mouse)
Modsite: RPHFPQFsysAsGtA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Disease tissue studied:  pancreatic cancer
Relevant cell lines - cell types - tissues:  pancreatic
Cellular systems studied:  tissue
Species studied:  mouse

S9-p - GSK3B (mouse)
Modsite: SGRPRttsFAEsCKP SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S9‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Disease tissue studied:  pancreatic cancer
Relevant cell lines - cell types - tissues:  pancreatic
Cellular systems studied:  tissue
Species studied:  mouse

S2448-p - mTOR (mouse)
Modsite: RsRtRtDsysAGQsV SwissProt Entrez-Gene
Orthologous residues
mTOR (human): S2448‑p, mTOR (mouse): S2448‑p, mTOR (rat): S2448‑p
Characterization
Methods used to characterize site in vivo phospho-antibody
Disease tissue studied:  pancreatic cancer
Relevant cell lines - cell types - tissues:  pancreatic
Cellular systems studied:  tissue
Species studied:  mouse