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Home > Curated Information Page > PubMed Id: 21441935
Liu X, et al. (2011) Intracellular MHC class II molecules promote TLR-triggered innate immune responses by maintaining activation of the kinase Btk. Nat Immunol 12, 416-24 21441935
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y223-p - Btk (human)
Modsite: LKKVVALyDyMPMNA SwissProt Entrez-Gene
Orthologous residues
Btk (human): Y223‑p, Btk iso2 (human): Y257‑p, Btk (mouse): Y223‑p, Btk (rat): Y224‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  macrophage-peritoneum
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Comments:  MHC class II-deficient (H2-/-) mice
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase reduced in H2(-/-) mice

Y551-p - Btk (human)
Modsite: RYVLDDEytsSVGSk SwissProt Entrez-Gene
Orthologous residues
Btk (human): Y551‑p, Btk iso2 (human): Y585‑p, Btk (mouse): Y551‑p, Btk (rat): Y552‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  macrophage-peritoneum
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Comments:  MHC class II-deficient (H2-/-) mice
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase reduced in H2(-/-) mice

T203-p - ERK1 (mouse)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  macrophage-peritoneum
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Comments:  MHC class II-deficient (H2-/-) mice
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase reduced in H2(-/-) mice

Y205-p - ERK1 (mouse)
Modsite: HtGFLtEyVAtRWyR SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  macrophage-peritoneum
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Comments:  MHC class II-deficient (H2-/-) mice
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase reduced in H2(-/-) mice

T183-p - ERK2 (mouse)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  macrophage-peritoneum
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Comments:  MHC class II-deficient (H2-/-) mice
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase reduced in H2(-/-) mice

Y185-p - ERK2 (mouse)
Modsite: HtGFLtEyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  macrophage-peritoneum
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Comments:  MHC class II-deficient (H2-/-) mice
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase reduced in H2(-/-) mice

S32-p - IkB-alpha (mouse)
Modsite: LVDDRHDsGLDsMKD SwissProt Entrez-Gene
Orthologous residues
IkB‑alpha (human): S32‑p, IkB‑alpha (mouse): S32‑p, IkB‑alpha (rat): S32‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  macrophage-peritoneum
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Comments:  MHC class II-deficient (H2-/-) mice
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase reduced in H2(-/-) mice

S36-p - IkB-alpha (mouse)
Modsite: RHDsGLDsMKDEEYE SwissProt Entrez-Gene
Orthologous residues
IkB‑alpha (human): S36‑p, IkB‑alpha (mouse): S36‑p, IkB‑alpha (rat): S36‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  macrophage-peritoneum
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Comments:  MHC class II-deficient (H2-/-) mice
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase reduced in H2(-/-) mice

S388-p - IRF3 (mouse)
Modsite: KTVDLHIsNSQPISL SwissProt Entrez-Gene
Orthologous residues
IRF3 (human): S396‑p, IRF3 (mouse): S388‑p, IRF3 (rat): S390‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  macrophage-peritoneum
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Comments:  MHC class II-deficient (H2-/-) mice
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase reduced in H2(-/-) mice

T183-p - JNK1 (mouse)
Modsite: AGtsFMMtPyVVtRY SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): T183‑p, JNK1 iso2 (human): T183‑p, JNK1 iso3 (human): T183‑p, JNK1 (mouse): T183‑p, JNK1 (rat): T183‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  macrophage-peritoneum
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Comments:  MHC class II-deficient (H2-/-) mice
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase reduced in H2(-/-) mice

Y185-p - JNK1 (mouse)
Modsite: tsFMMtPyVVtRYYR SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): Y185‑p, JNK1 iso2 (human): Y185‑p, JNK1 iso3 (human): Y185‑p, JNK1 (mouse): Y185‑p, JNK1 (rat): Y185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  macrophage-peritoneum
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Comments:  MHC class II-deficient (H2-/-) mice
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase reduced in H2(-/-) mice

T180-p - P38A (mouse)
Modsite: RHtDDEMtGyVAtRW SwissProt Entrez-Gene
Orthologous residues
P38A (human): T180‑p, P38A iso2 (human): T180‑p, P38A (mouse): T180‑p, P38A iso3 (mouse): T180‑p, P38A (rat): T180‑p, P38A (salmonid): T181‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  macrophage-peritoneum
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Comments:  MHC class II-deficient (H2-/-) mice
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase reduced in H2(-/-) mice

Y182-p - P38A (mouse)
Modsite: tDDEMtGyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
P38A (human): Y182‑p, P38A iso2 (human): Y182‑p, P38A (mouse): Y182‑p, P38A iso3 (mouse): Y182‑p, P38A (rat): Y182‑p, P38A (salmonid): Y183‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  macrophage-peritoneum
Cellular systems studied:  primary cultured cells
Species studied:  mouse
Comments:  MHC class II-deficient (H2-/-) mice
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase reduced in H2(-/-) mice