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Home > Curated Information Page > PubMed Id: 21423803
Schiappacassi M, et al. (2011) Role of T198 modification in the regulation of p27(Kip1) protein stability and function. PLoS One 6, e17673 21423803
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y74-p - p27Kip1 (human)
Modsite: HkPLEGKyEWQEVEk SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): Y74‑p, p27Kip1 (mouse): Y74‑p, p27Kip1 (rat): Y74‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Disease tissue studied:  fibrosarcoma of soft tissue
Relevant cell lines - cell types - tissues:  HT1080 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  protein degradation

Y88-p - p27Kip1 (human)
Modsite: kGsLPEFyyRPPRPP SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): Y88‑p, p27Kip1 (mouse): Y88‑p, p27Kip1 (rat): Y88‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Disease tissue studied:  fibrosarcoma of soft tissue
Relevant cell lines - cell types - tissues:  HT1080 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  protein degradation

T187-p - p27Kip1 (human)
Modsite: NAGsVEQtPKKPGLR SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): T187‑p, p27Kip1 (mouse): T187‑p, p27Kip1 (rat): T187‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Disease tissue studied:  brain cancer, glioblastoma, glioma, ovarian cancer, cancer, squamous cell carcinoma
Relevant cell lines - cell types - tissues:  MDAH2774 (ovarian), SCC-9 (squamous), U87MG (glial)
Cellular systems studied:  cell lines
Species studied:  human

T198-p - p27Kip1 (human)
Modsite: PGLRRRQt_______ SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): T198‑p, p27Kip1 (mouse): T197‑p, p27Kip1 (rat): T197‑p
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, mutation of modification site, western blotting
Disease tissue studied:  brain cancer, glioblastoma, glioma, ovarian cancer, fibrosarcoma of soft tissue, cancer, squamous cell carcinoma
Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast), E.coli (bacterial), HT1080 (fibroblast), MDAH2774 (ovarian), SCC-9 (squamous), U87MG (glial)
Cellular systems studied:  cell lines
Species studied:  bacteria, human
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  cell motility, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
STMN1 (human) Induces cell motility, induced co-immunoprecipitation, microscopy-colocalization