Curated Information
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Home > Curated Information Page > PubMed Id: 21795688
Pöll F, Doll C, Schulz S (2011) Rapid Dephosphorylation of G Protein-coupled Receptors by Protein Phosphatase 1{beta} Is Required for Termination of {beta}-Arrestin-dependent Signaling. J Biol Chem 286, 32931-6 21795688
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S341-p - SSTR2 (rat)
Modsite: GAEDGERsDsKQDKs SwissProt
Orthologous residues
SSTR2 (human): S341‑p, SSTR2 (mouse): S341‑p, SSTR2 (rat): S341‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

S343-p - SSTR2 (rat)
Modsite: EDGERsDsKQDKsRL SwissProt
Orthologous residues
SSTR2 (human): S343‑p, SSTR2 (mouse): S343‑p, SSTR2 (rat): S343‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human

T353-p - SSTR2 (rat)
Modsite: DKsRLNEttEtQRtL SwissProt
Orthologous residues
SSTR2 (human): T353‑p, SSTR2 (mouse): T353‑p, SSTR2 (rat): T353‑p
Characterization
Methods used to characterize site in vivo immunoassay, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PPP1CB (human) inhibition of upstream enzyme, siRNA inhibition of enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
calyculin_A, SST14 no change compared to control
okadaic_acid, SST14 decrease
Downstream Regulation
Effect of modification (function):  activity, induced, intracellular localization
Effect of modification (process):  signaling pathway regulation
Comments:  Dephosphorylation is initiated directly after receptor activation at or near the plasma membrane; leads to disruption of the ß-arrestin-GPCR complex

T354-p - SSTR2 (rat)
Modsite: KsRLNEttEtQRtLL SwissProt
Orthologous residues
SSTR2 (human): T354‑p, SSTR2 (mouse): T354‑p, SSTR2 (rat): T354‑p
Characterization
Methods used to characterize site in vivo immunoassay, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PPP1CB (human) inhibition of upstream enzyme, siRNA inhibition of enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
calyculin_A, SST14 no change compared to control
okadaic_acid, SST14 decrease
Downstream Regulation
Effect of modification (function):  activity, induced, intracellular localization
Effect of modification (process):  signaling pathway regulation
Comments:  Dephosphorylation is initiated directly after receptor activation at or near the plasma membrane; leads to disruption of the ß-arrestin-GPCR complex

T356-p - SSTR2 (rat)
Modsite: RLNEttEtQRtLLNG SwissProt
Orthologous residues
SSTR2 (human): T356‑p, SSTR2 (mouse): T356‑p, SSTR2 (rat): T356‑p
Characterization
Methods used to characterize site in vivo immunoassay, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PPP1CB (human) inhibition of upstream enzyme, siRNA inhibition of enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
calyculin_A, SST14 no change compared to control
okadaic_acid, SST14 decrease
Downstream Regulation
Effect of modification (function):  activity, induced, intracellular localization
Effect of modification (process):  signaling pathway regulation
Comments:  Dephosphorylation is initiated directly after receptor activation at or near the plasma membrane; leads to disruption of the ß-arrestin-GPCR complex

T359-p - SSTR2 (rat)
Modsite: EttEtQRtLLNGDLQ SwissProt
Orthologous residues
SSTR2 (human): T359‑p, SSTR2 (mouse): T359‑p, SSTR2 (rat): T359‑p
Characterization
Methods used to characterize site in vivo immunoassay, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
PHOSPHATASE PPP1CB (human) inhibition of upstream enzyme, siRNA inhibition of enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
calyculin_A, SST14 no change compared to control
okadaic_acid, SST14 decrease
Downstream Regulation
Effect of modification (function):  activity, induced, intracellular localization
Effect of modification (process):  signaling pathway regulation
Comments:  Dephosphorylation is initiated directly after receptor activation at or near the plasma membrane; leads to disruption of the ß-arrestin-GPCR complex