Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 21775625
Sato K, et al. (2011) Numb controls E-cadherin endocytosis through p120 catenin with aPKC. Mol Biol Cell 22, 3103-3119 21775625
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

S7-p - NUMB iso3 (human)
Modsite: _MNKLRQsFRRKKDV SwissProt Entrez-Gene
Orthologous residues
NUMB (human): S7‑p, NUMB iso2 (human): S7‑p, NUMB iso3 (human): S7‑p, NUMB (mouse): S7‑p, NUMB iso2 (mouse): S7‑p, NUMB (rat): S7‑p
Characterization
Methods used to characterize site in vivo immunoassay, mutation of modification site, phospho-antibody
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-7 (breast cell), MDCKII (epithelial)
Cellular systems studied:  cell lines
Species studied:  dog, human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKCZ (human) microscopy-colocalization, siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme
Downstream Regulation
Effect of modification (function):  intracellular localization, molecular association, regulation
Effect of modification (process):  endocytosis, inhibited
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
CTNND1 (human) Disrupts co-immunoprecipitation
AP2A1 (human) Disrupts co-immunoprecipitation
CDH1 (human) Disrupts co-immunoprecipitation

S265-p - NUMB iso3 (human)
Modsite: EQLARQGsFRGFPAL SwissProt Entrez-Gene
Orthologous residues
NUMB (human): S276‑p, NUMB iso2 (human): S276‑p, NUMB iso3 (human): S265‑p, NUMB (mouse): S276‑p, NUMB iso2 (mouse): S276‑p, NUMB (rat): S275‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-7 (breast cell), MDCKII (epithelial)
Cellular systems studied:  cell lines
Species studied:  dog, human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKCZ (human) microscopy-colocalization, siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme
Downstream Regulation
Effect of modification (function):  intracellular localization, molecular association, regulation
Effect of modification (process):  endocytosis, inhibited
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
CTNND1 (human) Disrupts co-immunoprecipitation
AP2A1 (human) Disrupts co-immunoprecipitation
CDH1 (human) Disrupts co-immunoprecipitation

S284-p - NUMB iso3 (human)
Modsite: sPFKRQLsLRINELP SwissProt Entrez-Gene
Orthologous residues
NUMB (human): S295‑p, NUMB iso2 (human): S295‑p, NUMB iso3 (human): S284‑p, NUMB (mouse): S295‑p, NUMB iso2 (mouse): S295‑p, NUMB (rat): S294‑p
Characterization
Methods used to characterize site in vivo mutation of modification site
Disease tissue studied:  breast cancer
Relevant cell lines - cell types - tissues:  MCF-7 (breast cell), MDCKII (epithelial)
Cellular systems studied:  cell lines
Species studied:  dog, human
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKCZ (human) microscopy-colocalization, siRNA inhibition of enzyme, pharmacological inhibitor of upstream enzyme
Downstream Regulation
Effect of modification (function):  intracellular localization, molecular association, regulation
Effect of modification (process):  endocytosis, inhibited
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
AP2A1 (human) Disrupts co-immunoprecipitation
CDH1 (human) Disrupts co-immunoprecipitation
CTNND1 (human) Disrupts co-immunoprecipitation