Curated Information
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Home > Curated Information Page > PubMed Id: 21724995
Guan YJ, et al. (2011) Phospho-SXXE/D Motif Mediated TNF Receptor 1-TRADD Death Domain Complex Formation for T Cell Activation and Migration. J Immunol 187, 1289-97 21724995
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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Y360-p - TNF-R1 (human)
Modsite: tDDPATLyAVVENVP SwissProt Entrez-Gene
Orthologous residues
TNF‑R1 (human): Y360‑p, TNF‑R1 (mouse): Y360‑p, TNF‑R1 (rat): Y367‑p

S381-p - TNF-R1 (human)
Modsite: FVRRLGLsDHEIDRL SwissProt Entrez-Gene
Orthologous residues
TNF‑R1 (human): S381‑p, TNF‑R1 (mouse): S381‑p, TNF‑R1 (rat): S388‑p
Characterization
Methods used to characterize site in vivo [32P] ATP in vitro, [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, western blotting
Disease tissue studied:  leukemia, T cell leukemia, lymphoma, Burkitt's lymphoma
Relevant cell lines - cell types - tissues:  Daudi (B lymphocyte), HEK293T (epithelial), HeLa (cervical), Jurkat (T lymphocyte), MEF (fibroblast), splenocyte, thymocyte
Cellular systems studied:  cell lines, primary cultured cells
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE IKKB (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE IKKB (human) genetic knockout/knockin of upstream enzyme, co-immunoprecipitation, pharmacological inhibitor of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
PS-1145 TNF inhibit treatment-induced increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  cell motility, induced, transcription, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
TRADD (human) Induces transcription, induced co-immunoprecipitation
Associated Diseases
Diseases Alterations Comments
Crohn disease, colonic inflamation increased

Y401-p - TNF-R1 (human)
Modsite: RCLREAQysMLAtWR SwissProt Entrez-Gene
Orthologous residues
TNF‑R1 (human): Y401‑p, TNF‑R1 (mouse): Y401‑p, TNF‑R1 (rat): Y408‑p

S215-p - TRADD (human)
Modsite: PVVNRPLsLKDQQTF SwissProt Entrez-Gene
Orthologous residues
TRADD (human): S215‑p, TRADD (mouse): T213‑p
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  transcription, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
FADD (human) Induces co-immunoprecipitation
TNF-R1 (human) Induces co-immunoprecipitation
RIP (human) Induces co-immunoprecipitation

S296-p - TRADD (human)
Modsite: LEENELTsLAEDLLG SwissProt Entrez-Gene
Orthologous residues
TRADD (human): S296‑p, TRADD (mouse): S294‑p
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  transcription, induced
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
FADD (human) Induces co-immunoprecipitation
RIP (human) Induces co-immunoprecipitation
TNF-R1 (human) Induces co-immunoprecipitation