Guan YJ, et al. (2011) Phospho-SXXE/D Motif Mediated TNF Receptor 1-TRADD Death Domain Complex Formation for T Cell Activation and Migration. J Immunol 187, 1289-97 21724995

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Y360-p - TNF-R1 (human) ▲

Modsite: tDDPATLyAVVENVP SwissProt Entrez-Gene Orthologous residues TNF‑R1 (human): Y360‑p, TNF‑R1 (mouse): Y360‑p, TNF‑R1 (rat): Y367‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Downstream Regulation Effect of modification (process):  transcription, induced

S381-p - TNF-R1 (human) ▲

Modsite: FVRRLGLsDHEIDRL SwissProt Entrez-Gene Orthologous residues TNF‑R1 (human): S381‑p, TNF‑R1 (mouse): S381‑p, TNF‑R1 (rat): S388‑p Characterization Methods used to characterize site in vivo:  [32P] ATP in vitro, [32P] bio-synthetic labeling, mutation of modification site, phospho-antibody, western blotting Disease tissue studied:  leukemia, T cell leukemia, lymphoma, Burkitt's lymphoma Relevant cell lines - cell types - tissues:  Daudi (B lymphocyte), HEK293T (epithelial), HeLa (cervical), Jurkat (T lymphocyte), MEF (fibroblast), splenocyte, thymocyte Cellular systems studied:  cell lines, primary cultured cells Species studied:  human, mouse Enzymes shown to modify site in vitro:  Type Enzyme KINASE IKKB (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE IKKB (human) pharmacological inhibitor of upstream enzyme, co-immunoprecipitation, genetic knockout/knockin of upstream enzyme Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes TNF increase PS-1145 TNF inhibit treatment-induced increase Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  cell motility, induced, transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays TRADD (human) Induces transcription, induced co-immunoprecipitation Associated Diseases Diseases Alterations Comments Crohn disease, colonic inflamation increased

Y401-p - TNF-R1 (human) ▲

Modsite: RCLREAQysMLAtWR SwissProt Entrez-Gene Orthologous residues TNF‑R1 (human): Y401‑p, TNF‑R1 (mouse): Y401‑p, TNF‑R1 (rat): Y408‑p Characterization Methods used to characterize site in vivo:  mutation of modification site Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Downstream Regulation Effect of modification (process):  transcription, induced

S215-p - TRADD (human) ▲

Modsite: PVVNRPLsLKDQQTF SwissProt Entrez-Gene Orthologous residues TRADD (human): S215‑p, TRADD (mouse): T213‑p, TRADD (rat): N213‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays TNF-R1 (human) Induces co-immunoprecipitation FADD (human) Induces co-immunoprecipitation RIP (human) Induces co-immunoprecipitation

S296-p - TRADD (human) ▲

Modsite: LEENELTsLAEDLLG SwissProt Entrez-Gene Orthologous residues TRADD (human): S296‑p, TRADD (mouse): S294‑p, TRADD (rat): S294‑p Characterization Methods used to characterize site in vivo:  [32P] bio-synthetic labeling, mutation of modification site Relevant cell lines - cell types - tissues:  HEK293T (epithelial) Cellular systems studied:  cell lines Species studied:  human Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays FADD (human) Induces co-immunoprecipitation RIP (human) Induces co-immunoprecipitation TNF-R1 (human) Induces co-immunoprecipitation