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Dunlop EA, et al. (2011) ULK1 inhibits mTORC1 signaling, promotes multisite Raptor phosphorylation and hinders substrate binding. Autophagy 7, 737-47 21460630
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S2481-p - mTOR (human)
Modsite: tVPEsIHsFIGDGLV SwissProt Entrez-Gene
Orthologous residues
mTOR (human): S2481‑p, mTOR (mouse): S2481‑p, mTOR (rat): S2481‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ULK1 (human) increase
KU-0063794 ULK1 (human) inhibit treatment-induced increase

S696-p - Raptor (human)
Modsite: EKNyALPsPAttEGG SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S696‑p, Raptor (mouse): S696‑p, Raptor (rat): S696‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
KU-0063794 no change compared to control

T706-p - Raptor (human)
Modsite: ttEGGsLtPVRDsPC SwissProt Entrez-Gene
Orthologous residues
Raptor (human): T706‑p, Raptor (mouse): T706‑p, Raptor (rat): T706‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
KU-0063794 no change compared to control

S792-p - Raptor (human)
Modsite: DKMRRAssYSsLNSL SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S792‑p, Raptor (mouse): S792‑p, Raptor (rat): S792‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ULK1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ULK1 (human) transfection of wild-type enzyme, siRNA inhibition of enzyme, transfection of inactive enzyme
KINASE ULK2 (human) transfection of wild-type enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
KU-0063794 no change compared to control
siRNA decrease ULK1-siRNA inhibits
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  signaling pathway regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
4E-BP1 (human) Disrupts far-Western
Comments:  mTORC1 signaling

S855-p - Raptor (human)
Modsite: QRVLDtssLtQsAPA SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S855‑p, Raptor (mouse): S855‑p, Raptor (rat): S855‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ULK1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ULK2 (human) transfection of wild-type enzyme
KINASE ULK1 (human) transfection of wild-type enzyme, siRNA inhibition of enzyme, transfection of inactive enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
KU-0063794 no change compared to control
siRNA decrease ULK1-siRNA inhibits
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  signaling pathway regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
4E-BP1 (human) Disrupts far-Western
Comments:  mTORC1 signaling

S859-p - Raptor (human)
Modsite: DtssLtQsAPAsPtN SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S859‑p, Raptor (mouse): S859‑p, Raptor (rat): S859‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ULK1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ULK2 (human) transfection of wild-type enzyme
KINASE ULK1 (human) transfection of wild-type enzyme, siRNA inhibition of enzyme, transfection of inactive enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
KU-0063794 no change compared to control
siRNA decrease ULK1-siRNA inhibits
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Effect of modification (process):  signaling pathway regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
4E-BP1 (human) Disrupts far-Western
Comments:  mTORC1 signaling

S863-p - Raptor (human)
Modsite: LtQsAPAsPtNkGVH SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S863‑p, Raptor (mouse): S863‑p, Raptor (rat): S863‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ULK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
KU-0063794 decrease

S877-p - Raptor (human)
Modsite: HIHQAGGsPPAssts SwissProt Entrez-Gene
Orthologous residues
Raptor (human): S877‑p, Raptor (mouse): S877‑p, Raptor (rat): S877‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ULK1 (human)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
KU-0063794 no change compared to control