Curated Information
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Home > Curated Information Page > PubMed Id: 20841359
Song B, et al. (2010) Inhibitory phosphorylation of GSK-3 by CaMKII couples depolarization to neuronal survival. J Biol Chem 285, 41122-34 20841359
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T308-p - Akt1 (rat)
Modsite: KDGATMKtFCGTPEy SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): T308‑p, Akt1 iso2 (human): T246‑p, Akt1 (mouse): T308‑p, Akt1 (rat): T308‑p, Akt1 (fruit fly): T423‑p, Akt1 (cow): T308‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  neuron-'brain, cerebral cortex'
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
depolarization increase
wortmannin depolarization inhibit treatment-induced increase
LY294002 depolarization inhibit treatment-induced increase
IGF-1 increase
wortmannin IGF-1 inhibit treatment-induced increase
LY294002 IGF-1 inhibit treatment-induced increase

S473-gl - Akt1 (rat)
Modsite: RPHFPQFsYSASGTA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑gl, Akt1 iso2 (human): S411‑gl, Akt1 (mouse): S473‑gl, Akt1 (rat): S473‑gl, Akt1 (fruit fly): S586‑gl, Akt1 (cow): S473‑gl
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  neuron-'brain, cerebral cortex'
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
depolarization increase
wortmannin depolarization inhibit treatment-induced increase
LY294002 depolarization inhibit treatment-induced increase
IGF-1 increase
wortmannin IGF-1 inhibit treatment-induced increase
LY294002 IGF-1 inhibit treatment-induced increase

T514-p - CRMP-2 (rat)
Modsite: SVtPKTVtPASsAKT SwissProt Entrez-Gene
Orthologous residues
CRMP‑2 (human): T514‑p, CRMP‑2 iso3 (human): T619‑p, CRMP‑2 (mouse): T514‑p, CRMP‑2 (rat): T514‑p, CRMP‑2 (chicken): T514‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  neuron-'brain, cerebral cortex'
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
depolarization increase
SB415286 depolarization inhibit treatment-induced increase
AR-A014418 depolarization inhibit treatment-induced increase

T32-p - FOXO3A (rat)
Modsite: QSRPRSCtWPLQRPE SwissProt Entrez-Gene
Orthologous residues
FOXO3A (human): T32‑p, FOXO3A (mouse): T32‑p, FOXO3A (rat): T32‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  neuron-'brain, cerebral cortex'
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
depolarization increase
wortmannin depolarization inhibit treatment-induced increase
LY294002 depolarization inhibit treatment-induced increase
IGF-1 increase
wortmannin IGF-1 inhibit treatment-induced increase
LY294002 IGF-1 inhibit treatment-induced increase

S21-p - GSK3A (rat)
Modsite: SGRARtssFAEPGGG SwissProt Entrez-Gene
Orthologous residues
GSK3A (human): S21‑p, GSK3A (mouse): S21‑p, GSK3A (rat): S21‑p, GSK3A (cow): S21‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  neuron-'brain, cerebral cortex'
Cellular systems studied:  primary cultured cells
Species studied:  rat
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2B (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CAMK2B (rat) pharmacological inhibitor of upstream enzyme, microscopy-colocalization, transfection of wild-type enzyme, co-immunoprecipitation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
depolarization increase
depolarization increase
wortmannin depolarization no effect upon treatment-induced increase
LY294002 depolarization inhibit treatment-induced increase
U0126 depolarization no effect upon treatment-induced increase
PD98059 depolarization no effect upon treatment-induced increase
PKI depolarization no effect upon treatment-induced increase
KN-62 depolarization inhibit treatment-induced increase
STO-609 depolarization no effect upon treatment-induced increase
IGF-1 increase
wortmannin IGF-1 no effect upon treatment-induced increase
LY294002 IGF-1 no effect upon treatment-induced increase

Y279-p - GSK3A (rat)
Modsite: RGEPNVsyICsRyYR SwissProt Entrez-Gene
Orthologous residues
GSK3A (human): Y279‑p, GSK3A (mouse): Y279‑p, GSK3A (rat): Y279‑p, GSK3A (cow): Y279‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  neuron-'brain, cerebral cortex'
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
depolarization increase

S9-p - GSK3B (rat)
Modsite: SGRPRTTsFAESCKP SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S9‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  neuron-'brain, cerebral cortex'
Cellular systems studied:  primary cultured cells
Species studied:  rat
Enzymes shown to modify site in vitro
Type Enzyme
KINASE CAMK2B (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE CAMK2B (rat) pharmacological inhibitor of upstream enzyme, microscopy-colocalization, transfection of wild-type enzyme, co-immunoprecipitation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
depolarization increase
depolarization increase
wortmannin depolarization no effect upon treatment-induced increase
LY294002 depolarization inhibit treatment-induced increase
U0126 depolarization no effect upon treatment-induced increase
PD98059 depolarization no effect upon treatment-induced increase
PKI depolarization no effect upon treatment-induced increase
KN-62 depolarization inhibit treatment-induced increase
STO-609 depolarization no effect upon treatment-induced increase
IGF-1 increase
wortmannin IGF-1 no effect upon treatment-induced increase
LY294002 IGF-1 no effect upon treatment-induced increase

Y216-p - GSK3B (rat)
Modsite: RGEPNVsyICsRyYR SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): Y216‑p, GSK3B iso2 (human): Y216‑p, GSK3B (mouse): Y216‑p, GSK3B (rat): Y216‑p, GSK3B (rabbit): Y216‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  neuron-'brain, cerebral cortex'
Cellular systems studied:  primary cultured cells
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
depolarization increase