Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 12621061
Kabotyanski EB, Rosen JM (2003) Signal transduction pathways regulated by prolactin and Src result in different conformations of activated Stat5b. J Biol Chem 278, 17218-27 12621061
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

Y679-p - STAT5B (rat)
Modsite: DRPKDEVySKYYTPV SwissProt Entrez-Gene
Orthologous residues
STAT5B (human): Y679‑p, STAT5B (mouse): Y679‑p, STAT5B (rat): Y679‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Src (human) increase
Downstream Regulation
Effect of modification (function):  intracellular localization
Effect of modification (process):  transcription, altered

Y699-p - STAT5B (rat)
Modsite: TAKAADGyVKPQIKQ SwissProt Entrez-Gene
Orthologous residues
STAT5B (human): Y699‑p, STAT5B (mouse): Y699‑p, STAT5B (rat): Y699‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Src (human) increase
Downstream Regulation
Effect of modification (function):  intracellular localization, molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Induces

Y724-p - STAT5B (rat)
Modsite: DAGSGATyMDQAPsP SwissProt Entrez-Gene
Orthologous residues
STAT5B (human): Y725‑p, STAT5B (mouse): Y724‑p, STAT5B (rat): Y724‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
Src (human) increase

S730-p - STAT5B (rat)
Modsite: TyMDQAPsPVVCPQA SwissProt Entrez-Gene
Orthologous residues
STAT5B (human): S731‑p, STAT5B (mouse): S730‑p, STAT5B (rat): S730‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical), MEF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PRL increase
Src (human) increase