Bradshaw NJ, et al. (2011) PKA Phosphorylation of NDE1 Is DISC1/PDE4 Dependent and Modulates Its Interaction with LIS1 and NDEL1. J Neurosci 31, 9043-54 21677187

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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T132-p - NDEL1 (human) ▲
Modsite: LERAKRAtIVsLEDF SwissProt Entrez-Gene Orthologous residues NDEL1 (human): T132‑p, NDEL1 iso2 (human): T132‑p, NDEL1 iso3 (human): T132‑p, NDEL1 (mouse): T132‑p, NDEL1 (rat): T132‑p

T131-p - NUDE1 iso1 (human) ▲

Modsite: LERAKRAtIMSLEDF SwissProt Entrez-Gene Orthologous residues NUDE1 (human): T131‑p, NUDE1 iso1 (human): T131‑p, NUDE1 (mouse): T131‑p, NUDE1 iso3 (mouse): T131‑p, NUDE1 (rat): T131‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody Disease tissue studied:  adrenal cancer, pheochromocytoma Relevant cell lines - cell types - tissues:  COS (fibroblast), PC-12 (chromaffin) Cellular systems studied:  cell lines Species studied:  green monkey Comments:  PC-12 neuronal cell line (NS-1) Enzymes shown to modify site in vitro:  Type Enzyme KINASE PKACA (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PKACA (human) phospho-motif antibody, pharmacological activator of upstream enzyme, modification site within consensus motif, mutation in upstream enzyme recognition motif Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes DISC1 (human) increase Downstream Regulation Effect of modification (function):  molecular association, regulation, protein conformation Effect of modification (process):  cytoskeletal reorganization Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays NDEL1 (human) Induces protein conformation co-immunoprecipitation PAFAH1B1 (human) Disrupts co-immunoprecipitation Comments:  phosphomimetic mutant inhibits neurite extension

S306-p - NUDE1 iso1 (human) ▲

Modsite: DGGERRPsstsVPLG SwissProt Entrez-Gene Orthologous residues NUDE1 (human): S306‑p, NUDE1 iso1 (human): S306‑p, NUDE1 (mouse): G306‑p, NUDE1 iso3 (mouse): G306‑p, NUDE1 (rat): G306‑p Characterization Methods used to characterize site in vivo:  mutation of modification site, phospho-antibody Disease tissue studied:  adrenal cancer, pheochromocytoma Relevant cell lines - cell types - tissues:  COS (fibroblast), PC-12 (chromaffin) Cellular systems studied:  cell lines Species studied:  green monkey Comments:  PC-12 neuronal cell line (NS-1) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE PKACA (human) phospho-motif antibody, pharmacological activator of upstream enzyme, modification site within consensus motif, mutation in upstream enzyme recognition motif Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes DISC1 (human) increase