Curated Information
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Home > Curated Information Page > PubMed Id: 11997497
Liu J, Kimura A, Baumann CA, Saltiel AR (2002) APS facilitates c-Cbl tyrosine phosphorylation and GLUT4 translocation in response to insulin in 3T3-L1 adipocytes. Mol Cell Biol 22, 3599-609 11997497
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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Y371-p - CBL (human)
Modsite: TQEQyELyCEMGSTF SwissProt Entrez-Gene
Orthologous residues
CBL (human): Y371‑p, CBL (mouse): Y369‑p, CBL (rat): Y370‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast), CHO (fibroblast) [EphB1 (human), transfection]
Cellular systems studied:  cell lines
Species studied:  hamster, mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE INSR (rat) phospho-motif antibody, pharmacological activator of upstream enzyme, co-immunoprecipitation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
PP2 insulin no effect upon treatment-induced increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
CRK (human) SH2 Induces co-immunoprecipitation

Y700-p - CBL (human)
Modsite: EGEEDtEyMtPssRP SwissProt Entrez-Gene
Orthologous residues
CBL (human): Y700‑p, CBL (mouse): Y698‑p, CBL (rat): Y699‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast), CHO (fibroblast) [EphB1 (human), transfection]
Cellular systems studied:  cell lines
Species studied:  hamster, mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE INSR (rat) phospho-motif antibody, pharmacological activator of upstream enzyme, co-immunoprecipitation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
PP2 insulin no effect upon treatment-induced increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
CRK (human) SH2 Induces co-immunoprecipitation

Y774-p - CBL (human)
Modsite: sENEDDGyDVPKPPV SwissProt Entrez-Gene
Orthologous residues
CBL (human): Y774‑p, CBL (mouse): Y780‑p, CBL (rat): Y781‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast), CHO (fibroblast) [EphB1 (human), transfection]
Cellular systems studied:  cell lines
Species studied:  hamster, mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE INSR (rat) phospho-motif antibody, pharmacological activator of upstream enzyme, co-immunoprecipitation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
PP2 insulin no effect upon treatment-induced increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
CRK (human) SH2 Induces co-immunoprecipitation

Y618-p - APS (rat)
Modsite: ARAVENQySFY____ SwissProt Entrez-Gene
Orthologous residues
APS (human): Y629‑p, APS iso2 (human): Y672‑p, APS (mouse): Y618‑p, APS (rat): Y618‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast), CHO (fibroblast) [EphB1 (human), transfection]
Cellular systems studied:  cell lines
Species studied:  hamster, mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE INSR (rat) phospho-motif antibody, pharmacological activator of upstream enzyme, co-immunoprecipitation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
Cbl-c (human) Induces molecular association, regulation, phosphorylation co-immunoprecipitation