Curated Information
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Home > Curated Information Page > PubMed Id: 12620228
Zhao J, Yuan X, Frödin M, Grummt I (2003) ERK-dependent phosphorylation of the transcription initiation factor TIF-IA is required for RNA polymerase I transcription and cell growth. Mol Cell 11, 405-13 12620228
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S633-p - TIF1A (human)
Modsite: SFDTHFRsPssSVGs SwissProt Entrez-Gene
Orthologous residues
TIF1A (human): S633‑p, TIF1A (mouse): S631‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ERK1 (human) pharmacological inhibitor of upstream enzyme, phosphoaminoacid analysis
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PD98059 decrease

S649-p - TIF1A (human)
Modsite: PVLYMQPsPL_____ SwissProt Entrez-Gene
Orthologous residues
TIF1A (human): S649‑p, TIF1A (mouse): S648‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, phosphopeptide mapping
Relevant cell lines - cell types - tissues:  293 (epithelial), 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout]
Cellular systems studied:  cell lines
Species studied:  human, mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE RSK2 (human) phosphoaminoacid analysis
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
PD98059 decrease
Downstream Regulation
Effect of modification (function):  activity, induced