Curated Information
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Home > Curated Information Page > PubMed Id: 10636859
Christophe T, et al. (2000) Human complement 5a (C5a) anaphylatoxin receptor (CD88) phosphorylation sites and their specific role in receptor phosphorylation and attenuation of G protein-mediated responses. Desensitization of C5a receptor controls superoxide production but not receptor sequestration in HL-60 cells. J Biol Chem 275, 1656-64 10636859
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S327-p - C5aR (human)
Modsite: RNVLtEEsVVREsKs SwissProt Entrez-Gene
Orthologous residues
C5aR (human): S327‑p, C5aR (mouse): S328‑p, C5aR iso3 (mouse): S328‑p, C5aR (rat): S329‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast), HL60 (myeloid)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
C5a increase

S332-p - C5aR (human)
Modsite: EEsVVREsKsFTRsT SwissProt Entrez-Gene
Orthologous residues
C5aR (human): S332‑p, C5aR (mouse): S333‑p, C5aR iso3 (mouse): S333‑p, C5aR (rat): S334‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast), HL60 (myeloid)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
C5a increase
Downstream Regulation
Effect of modification (function):  receptor desensitization, altered

S334-p - C5aR (human)
Modsite: sVVREsKsFTRsTVD SwissProt Entrez-Gene
Orthologous residues
C5aR (human): S334‑p, C5aR (mouse): T335‑p, C5aR iso3 (mouse): T335‑p, C5aR (rat): S336‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast), HL60 (myeloid)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
C5a increase
Downstream Regulation
Effect of modification (function):  receptor desensitization, altered

S338-p - C5aR (human)
Modsite: EsKsFTRsTVDTMAQ SwissProt Entrez-Gene
Orthologous residues
C5aR (human): S338‑p, C5aR (mouse): S339‑p, C5aR iso3 (mouse): S339‑p, C5aR (rat): S340‑p
Characterization
Methods used to characterize site in vivo [32P] bio-synthetic labeling, mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  COS (fibroblast), HL60 (myeloid)
Cellular systems studied:  cell lines
Species studied:  green monkey, human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
C5a increase
Downstream Regulation
Effect of modification (function):  receptor desensitization, altered