Curated Information
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Home > Curated Information Page > PubMed Id: 12464600
Braun L, Christophe T, Boulay F (2003) Phosphorylation of key serine residues is required for internalization of the complement 5a (C5a) anaphylatoxin receptor via a beta-arrestin, dynamin, and clathrin-dependent pathway. J Biol Chem 278, 4277-85 12464600
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S314-p - C5aR (human)
Modsite: FQGRLRKsLPsLLRN SwissProt Entrez-Gene
Orthologous residues
C5aR (human): S314‑p, C5aR (mouse): S315‑p, C5aR iso3 (mouse): S315‑p, C5aR (rat): S316‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  RIN (epithelial)
Cellular systems studied:  cell lines
Species studied:  rat
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
ARRB1 (human) Induces molecular association, regulation chemical cross-linking
ARRB2 (human) Induces molecular association, regulation chemical cross-linking

S317-p - C5aR (human)
Modsite: RLRKsLPsLLRNVLt SwissProt Entrez-Gene
Orthologous residues
C5aR (human): S317‑p, C5aR (mouse): S318‑p, C5aR iso3 (mouse): S318‑p, C5aR (rat): S319‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  RIN (epithelial)
Cellular systems studied:  cell lines
Species studied:  rat
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
ARRB2 (human) Induces molecular association, regulation chemical cross-linking
ARRB1 (human) Induces molecular association, regulation chemical cross-linking

S327-p - C5aR (human)
Modsite: RNVLtEEsVVREsKs SwissProt Entrez-Gene
Orthologous residues
C5aR (human): S327‑p, C5aR (mouse): S328‑p, C5aR iso3 (mouse): S328‑p, C5aR (rat): S329‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  RIN (epithelial)
Cellular systems studied:  cell lines
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
C5a increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
ARRB2 (human) Induces molecular association, regulation chemical cross-linking
ARRB1 (human) Induces molecular association, regulation chemical cross-linking

S332-p - C5aR (human)
Modsite: EEsVVREsKsFTRsT SwissProt Entrez-Gene
Orthologous residues
C5aR (human): S332‑p, C5aR (mouse): S333‑p, C5aR iso3 (mouse): S333‑p, C5aR (rat): S334‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  RIN (epithelial)
Cellular systems studied:  cell lines
Species studied:  rat
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
ARRB2 (human) Induces molecular association, regulation, intracellular localization microscopy-colocalization
ARRB1 (human) Induces molecular association, regulation, intracellular localization microscopy-colocalization
ARRB2 (human) Induces molecular association, regulation chemical cross-linking
ARRB1 (human) Induces molecular association, regulation chemical cross-linking

S334-p - C5aR (human)
Modsite: sVVREsKsFTRsTVD SwissProt Entrez-Gene
Orthologous residues
C5aR (human): S334‑p, C5aR (mouse): T335‑p, C5aR iso3 (mouse): T335‑p, C5aR (rat): S336‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  RIN (epithelial)
Cellular systems studied:  cell lines
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
C5a increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
ARRB1 (human) Induces molecular association, regulation, intracellular localization microscopy-colocalization
ARRB2 (human) Induces molecular association, regulation chemical cross-linking
ARRB1 (human) Induces molecular association, regulation chemical cross-linking
ARRB2 (human) Induces molecular association, regulation, intracellular localization microscopy-colocalization

S338-p - C5aR (human)
Modsite: EsKsFTRsTVDTMAQ SwissProt Entrez-Gene
Orthologous residues
C5aR (human): S338‑p, C5aR (mouse): S339‑p, C5aR iso3 (mouse): S339‑p, C5aR (rat): S340‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, western blotting
Relevant cell lines - cell types - tissues:  RIN (epithelial)
Cellular systems studied:  cell lines
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
C5a increase
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
ARRB1 (human) Induces molecular association, regulation chemical cross-linking
ARRB2 (human) Induces molecular association, regulation chemical cross-linking
ARRB1 (human) Induces molecular association, regulation, intracellular localization microscopy-colocalization
ARRB2 (human) Induces molecular association, regulation, intracellular localization microscopy-colocalization