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Home > Curated Information Page > PubMed Id: 16565486
Ohi N, et al. (2006) Maintenance of Bad phosphorylation prevents apoptosis of rat hepatic sinusoidal endothelial cells in vitro and in vivo. Am J Pathol 168, 1097-106 16565486
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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S376-p - MSK1 (human)
Modsite: EKLFQGysFVAPsIL SwissProt Entrez-Gene
Orthologous residues
MSK1 (human): S376‑p, MSK1 (mouse): S375‑p, MSK1 (rat): S329‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  liver
Cellular systems studied:  primary cells
Species studied:  rat
Comments:  Rat liver sinusoidal endothelial cells (SECs)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
culturing_of_cells decrease 48 hours in culture (after removal from liver)
VEGF culturing_of_cells no effect upon treatment-induced decrease
vanadate culturing_of_cells inhibit treatment-induced decrease
vanadate increase From 48 hour cultured cells
LY294002 vanadate no effect upon treatment-induced increase
PD98059 vanadate inhibit treatment-induced increase
SB202190 vanadate inhibit treatment-induced increase
SB203580 vanadate inhibit treatment-induced increase
ischemia/reperfusion decrease
vanadate ischemia/reperfusion inhibit treatment-induced decrease

S473-p - Akt1 (rat)
Modsite: RPHFPQFsYSASGTA SwissProt Entrez-Gene
Orthologous residues
Akt1 (human): S473‑p, Akt1 iso2 (human): S411‑p, Akt1 (mouse): S473‑p, Akt1 (rat): S473‑p, Akt1 (fruit fly): S586‑p, Akt1 (cow): S473‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  liver
Cellular systems studied:  primary cells
Species studied:  rat
Comments:  Rat liver sinusoidal endothelial cells (SECs)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
culturing_of_cells decrease 48 hours in culture (after removal from liver)
VEGF culturing_of_cells no effect upon treatment-induced decrease
vanadate culturing_of_cells inhibit treatment-induced decrease
vanadate increase From 48 hour cultured cells
LY294002 vanadate inhibit treatment-induced increase
PD98059 vanadate no effect upon treatment-induced increase
SB202190 vanadate inhibit treatment-induced increase
SB203580 vanadate inhibit treatment-induced increase
ischemia/reperfusion decrease
vanadate ischemia/reperfusion inhibit treatment-induced decrease

S113-p - BAD (rat)
Modsite: ETRSRHSsYPAGtEE SwissProt Entrez-Gene
Orthologous residues
BAD (human): S75‑p, BAD (mouse): S112‑p, BAD (rat): S113‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  liver
Cellular systems studied:  primary cells
Species studied:  rat
Comments:  Rat liver sinusoidal endothelial cells (SECs)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
culturing_of_cells decrease 48 hours in culture (after removal from liver)
VEGF culturing_of_cells no effect upon treatment-induced decrease
vanadate culturing_of_cells inhibit treatment-induced decrease
vanadate increase From 48 hour cultured cells
LY294002 vanadate no effect upon treatment-induced increase
PD98059 vanadate inhibit treatment-induced increase
SB202190 vanadate inhibit treatment-induced increase
SB203580 vanadate inhibit treatment-induced increase
ischemia/reperfusion decrease
vanadate ischemia/reperfusion inhibit treatment-induced decrease

S137-p - BAD (rat)
Modsite: PFRGRsRsAPPNLWA SwissProt Entrez-Gene
Orthologous residues
BAD (human): S99‑p, BAD (mouse): S136‑p, BAD (rat): S137‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  liver
Cellular systems studied:  primary cells
Species studied:  rat
Comments:  Rat liver sinusoidal endothelial cells (SECs)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
culturing_of_cells decrease 48 hours in culture (after removal from liver)
VEGF culturing_of_cells no effect upon treatment-induced decrease
vanadate culturing_of_cells inhibit treatment-induced decrease
vanadate increase From 48 hour cultured cells
LY294002 vanadate inhibit treatment-induced increase
PD98059 vanadate no effect upon treatment-induced increase
SB202190 vanadate inhibit treatment-induced increase
SB203580 vanadate inhibit treatment-induced increase
ischemia/reperfusion decrease
vanadate ischemia/reperfusion inhibit treatment-induced decrease

T203-p - ERK1 (rat)
Modsite: HDHTGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  liver
Cellular systems studied:  primary cells
Species studied:  rat
Comments:  Rat liver sinusoidal endothelial cells (SECs)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
culturing_of_cells decrease 48 hours in culture (after removal from liver)
VEGF culturing_of_cells no effect upon treatment-induced decrease
vanadate culturing_of_cells inhibit treatment-induced decrease
vanadate increase From 48 hour cultured cells
LY294002 vanadate no effect upon treatment-induced increase
PD98059 vanadate inhibit treatment-induced increase
SB202190 vanadate no effect upon treatment-induced increase
SB203580 vanadate no effect upon treatment-induced increase
ischemia/reperfusion decrease
vanadate ischemia/reperfusion inhibit treatment-induced decrease

Y205-p - ERK1 (rat)
Modsite: HTGFLtEyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  liver
Cellular systems studied:  primary cells
Species studied:  rat
Comments:  Rat liver sinusoidal endothelial cells (SECs)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
culturing_of_cells decrease 48 hours in culture (after removal from liver)
VEGF culturing_of_cells no effect upon treatment-induced decrease
vanadate culturing_of_cells inhibit treatment-induced decrease
vanadate increase From 48 hour cultured cells
LY294002 vanadate no effect upon treatment-induced increase
PD98059 vanadate inhibit treatment-induced increase
SB202190 vanadate no effect upon treatment-induced increase
SB203580 vanadate no effect upon treatment-induced increase
ischemia/reperfusion decrease
vanadate ischemia/reperfusion inhibit treatment-induced decrease

T183-p - ERK2 (rat)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  liver
Cellular systems studied:  primary cells
Species studied:  rat
Comments:  Rat liver sinusoidal endothelial cells (SECs)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
culturing_of_cells decrease 48 hours in culture (after removal from liver)
VEGF culturing_of_cells no effect upon treatment-induced decrease
vanadate culturing_of_cells inhibit treatment-induced decrease
vanadate increase From 48 hour cultured cells
LY294002 vanadate no effect upon treatment-induced increase
PD98059 vanadate inhibit treatment-induced increase
SB202190 vanadate no effect upon treatment-induced increase
SB203580 vanadate no effect upon treatment-induced increase
ischemia/reperfusion decrease
vanadate ischemia/reperfusion inhibit treatment-induced decrease

Y185-p - ERK2 (rat)
Modsite: HtGFLtEyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  liver
Cellular systems studied:  primary cells
Species studied:  rat
Comments:  Rat liver sinusoidal endothelial cells (SECs)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
culturing_of_cells decrease 48 hours in culture (after removal from liver)
VEGF culturing_of_cells no effect upon treatment-induced decrease
vanadate culturing_of_cells inhibit treatment-induced decrease
vanadate increase From 48 hour cultured cells
LY294002 vanadate no effect upon treatment-induced increase
PD98059 vanadate inhibit treatment-induced increase
SB202190 vanadate no effect upon treatment-induced increase
SB203580 vanadate no effect upon treatment-induced increase
ischemia/reperfusion decrease
vanadate ischemia/reperfusion inhibit treatment-induced decrease

T180-p - P38A (rat)
Modsite: RHTDDEMtGyVATRW SwissProt Entrez-Gene
Orthologous residues
P38A (human): T180‑p, P38A iso2 (human): T180‑p, P38A (mouse): T180‑p, P38A iso3 (mouse): T180‑p, P38A (rat): T180‑p, P38A (salmonid): T181‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  liver
Cellular systems studied:  primary cells
Species studied:  rat
Comments:  Rat liver sinusoidal endothelial cells (SECs)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
culturing_of_cells decrease 48 hours in culture (after removal from liver)
VEGF culturing_of_cells no effect upon treatment-induced decrease
vanadate culturing_of_cells inhibit treatment-induced decrease
vanadate increase From 48 hour cultured cells
LY294002 vanadate no effect upon treatment-induced increase
PD98059 vanadate no effect upon treatment-induced increase
SB202190 vanadate inhibit treatment-induced increase
SB203580 vanadate inhibit treatment-induced increase
ischemia/reperfusion no change compared to control
vanadate ischemia/reperfusion no change compared to control

Y182-p - P38A (rat)
Modsite: TDDEMtGyVATRWYR SwissProt Entrez-Gene
Orthologous residues
P38A (human): Y182‑p, P38A iso2 (human): Y182‑p, P38A (mouse): Y182‑p, P38A iso3 (mouse): Y182‑p, P38A (rat): Y182‑p, P38A (salmonid): Y183‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  liver
Cellular systems studied:  primary cells
Species studied:  rat
Comments:  Rat liver sinusoidal endothelial cells (SECs)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
culturing_of_cells decrease 48 hours in culture (after removal from liver)
VEGF culturing_of_cells no effect upon treatment-induced decrease
vanadate culturing_of_cells inhibit treatment-induced decrease
vanadate increase From 48 hour cultured cells
LY294002 vanadate no effect upon treatment-induced increase
PD98059 vanadate no effect upon treatment-induced increase
SB202190 vanadate inhibit treatment-induced increase
SB203580 vanadate inhibit treatment-induced increase
ischemia/reperfusion no change compared to control
vanadate ischemia/reperfusion no change compared to control

T359-p - p90RSK (rat)
Modsite: DTEFTSRtPRDsPGI SwissProt Entrez-Gene
Orthologous residues
p90RSK (human): T359‑p, p90RSK iso2 (human): T368‑p, p90RSK (mouse): T348‑p, p90RSK iso3 (mouse): , p90RSK (rat): T359‑p, p90RSK (chicken): T377‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  liver
Cellular systems studied:  primary cells
Species studied:  rat
Comments:  Rat liver sinusoidal endothelial cells (SECs)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
culturing_of_cells decrease 48 hours in culture (after removal from liver)
VEGF culturing_of_cells no effect upon treatment-induced decrease
vanadate culturing_of_cells inhibit treatment-induced decrease
vanadate increase From 48 hour cultured cells
LY294002 vanadate no effect upon treatment-induced increase
PD98059 vanadate inhibit treatment-induced increase
SB202190 vanadate no effect upon treatment-induced increase
SB203580 vanadate no effect upon treatment-induced increase
ischemia/reperfusion decrease
vanadate ischemia/reperfusion inhibit treatment-induced decrease

S363-p - p90RSK (rat)
Modsite: TSRtPRDsPGIPPsA SwissProt Entrez-Gene
Orthologous residues
p90RSK (human): S363‑p, p90RSK iso2 (human): S372‑p, p90RSK (mouse): S352‑p, p90RSK iso3 (mouse): , p90RSK (rat): S363‑p, p90RSK (chicken): S381‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  liver
Cellular systems studied:  primary cells
Species studied:  rat
Comments:  Rat liver sinusoidal endothelial cells (SECs)
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
culturing_of_cells decrease 48 hours in culture (after removal from liver)
VEGF culturing_of_cells no effect upon treatment-induced decrease
vanadate culturing_of_cells inhibit treatment-induced decrease
vanadate increase From 48 hour cultured cells
LY294002 vanadate no effect upon treatment-induced increase
PD98059 vanadate inhibit treatment-induced increase
SB202190 vanadate no effect upon treatment-induced increase
SB203580 vanadate no effect upon treatment-induced increase
ischemia/reperfusion decrease
vanadate ischemia/reperfusion inhibit treatment-induced decrease