Saitoh M, et al. (2002) Regulation of an activated S6 kinase 1 variant reveals a novel mammalian target of rapamycin phosphorylation site. J Biol Chem 277, 20104-12 11914378

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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T252-p - p70S6K (human) ▲

Modsite: HDGtVtHtFCGtIEY SwissProt Entrez-Gene Orthologous residues p70S6K (human): T252‑p, p70S6K iso2 (human): T229‑p, p70S6K (mouse): T252‑p, p70S6K (rat): T252‑p, p70S6K iso2 (rat): T229‑p, p70S6K (fruit fly): T238‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes insulin increase wortmannin insulin inhibit treatment-induced increase rapamycin insulin inhibit treatment-induced increase

S394-p - p70S6K (human) ▲

Modsite: TRQtPVDsPDDStLS SwissProt Entrez-Gene Orthologous residues p70S6K (human): S394‑p, p70S6K iso2 (human): S371‑p, p70S6K (mouse): S394‑p, p70S6K (rat): S394‑p, p70S6K iso2 (rat): S371‑p, p70S6K (fruit fly): S380‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, phospho-antibody, western blotting Relevant cell lines - cell types - tissues:  293 (epithelial) Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE mTOR (human) Upstream Regulation Potential in vivo enzymes for site:  Type Enzyme Evidence Notes KINASE mTOR (human) genetic transfer of wild-type enzyme, genetic transfer of dominant-negative enzyme, phospho-antibody Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes insulin increase wortmannin insulin inhibit treatment-induced increase rapamycin insulin inhibit treatment-induced increase serum increase rapamycin serum inhibit treatment-induced increase Downstream Regulation Effect of modification (function):  activity, induced

T412-p - p70S6K (human) ▲
Modsite: NQVFLGFtyVAPsVL SwissProt Entrez-Gene Orthologous residues p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p