Curated Information
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Home > Curated Information Page > PubMed Id: 18215134
Zhou QL, et al. (2008) Akt substrate TBC1D1 regulates GLUT1 expression through the mTOR pathway in 3T3-L1 adipocytes. Biochem J 411, 647-55 18215134
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T412-p - p70S6K (mouse)
Modsite: NQVFLGFtYVAPSVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast), CHO (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
siRNA TBC1D1 (mouse) increase
insulin increase
siRNA insulin TBC1D1 (mouse) no effect upon treatment-induced increase

S235-p - S6 (mouse)
Modsite: IAKRRRLssLRAsts SwissProt Entrez-Gene
Orthologous residues
S6 (human): S235‑p, S6 (mouse): S235‑p, S6 (rat): S235‑p, S6 (fruit fly): A234‑p, S6 (cow): S235‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast), CHO (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
siRNA TBC1D1 (mouse) increase
insulin increase
siRNA insulin TBC1D1 (mouse) no effect upon treatment-induced increase
rapamycin decrease

S236-p - S6 (mouse)
Modsite: AKRRRLssLRAstsK SwissProt Entrez-Gene
Orthologous residues
S6 (human): S236‑p, S6 (mouse): S236‑p, S6 (rat): S236‑p, S6 (fruit fly): S235‑p, S6 (cow): S236‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast), CHO (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster, mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
siRNA TBC1D1 (mouse) increase
insulin increase
siRNA insulin TBC1D1 (mouse) no effect upon treatment-induced increase
rapamycin decrease

T590-p - TBC1D1 (mouse)
Modsite: AFRRRANtLsHFPVE SwissProt Entrez-Gene
Orthologous residues
TBC1D1 (human): T596‑p, TBC1D1 iso2 (human): T596‑p, TBC1D1 (mouse): T590‑p, TBC1D1 iso2 (mouse): T590‑p, TBC1D1 (rat): T590‑p
Characterization
Methods used to characterize site in vivo immunoprecipitation, mass spectrometry, mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  3T3-L1 (fibroblast), CHO (fibroblast)
Cellular systems studied:  cell lines
Species studied:  hamster, mouse
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (mouse) modification site within consensus motif, phospho-motif antibody, pharmacological activator of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
insulin increase
Downstream Regulation
Effect of modification (function):  phosphorylation
Comments:  p70S6K phosphorylation