Li JG, Haines DS, Liu-Chen LY (2008) Agonist-promoted Lys63-linked polyubiquitination of the human kappa-opioid receptor is involved in receptor down-regulation. Mol Pharmacol 73, 1319-30 18212250

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

Click on the protein name to open the protein page, and on the RSD number to open the site page.

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K338-ub - KOR-1 (human) ▲

Modsite: AFLDENFkRCFRDFC SwissProt Entrez-Gene Orthologous residues KOR‑1 (human): K338‑ub, KOR‑1 (mouse): K338‑ub, KOR‑1 (rat): K338‑ub Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  CHO (fibroblast) Cellular systems studied:  cell lines Species studied:  hamster Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes U50488 increase dynorphin_A increase dynorphin_A CYLD (human) inhibit treatment-induced increase Downstream Regulation Effect of modification (function):  protein degradation, receptor internalization, induced Comments:  ubiquitination regulates but is not required for receptor downregulation

K349-ub - KOR-1 (human) ▲

Modsite: RDFCFPLkMRMERQS SwissProt Entrez-Gene Orthologous residues KOR‑1 (human): K349‑ub, KOR‑1 (mouse): K349‑ub, KOR‑1 (rat): K349‑ub Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  CHO (fibroblast) Cellular systems studied:  cell lines Species studied:  hamster Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes U50488 increase dynorphin_A increase dynorphin_A CYLD (human) inhibit treatment-induced increase Downstream Regulation Effect of modification (function):  protein degradation, receptor internalization, induced Comments:  ubiquitination regulates but is not required for receptor downregulation

S358-p - KOR-1 (human) ▲

Modsite: RMERQSTsRVRNTVQ SwissProt Entrez-Gene Orthologous residues KOR‑1 (human): S358‑p, KOR‑1 (mouse): N358‑p, KOR‑1 (rat): N358‑p Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  CHO (fibroblast) Cellular systems studied:  cell lines Species studied:  hamster Downstream Regulation Effect of modification (function):  ubiquitination Comments:  S358 phosphorylation enhances KOR-1 ubiquitination

K378-ub - KOR-1 (human) ▲

Modsite: RDIDGMNkPV_____ SwissProt Entrez-Gene Orthologous residues KOR‑1 (human): K378‑ub, KOR‑1 (mouse): K378‑ub, KOR‑1 (rat): K378‑ub Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  CHO (fibroblast) Cellular systems studied:  cell lines Species studied:  hamster Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes U50488 increase dynorphin_A increase dynorphin_A CYLD (human) inhibit treatment-induced increase Downstream Regulation Effect of modification (function):  protein degradation, receptor internalization, induced Comments:  ubiquitination regulates but is not required for receptor downregulation

K63-ub - ubiquitin (human) ▲

Modsite: LsDyNIQkEstLHLV SwissProt Orthologous residues ubiquitin (human): K63‑ub, ubiquitin (mouse): K63‑ub, ubiquitin (rat): K63‑ub, ubiquitin (cow): K63‑ub Characterization Methods used to characterize site in vivo:  immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  CHO (fibroblast) Cellular systems studied:  cell lines Species studied:  hamster Upstream Regulation Treatments, proteins and their effect on site modification:  Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes U50488 increase dynorphin_A increase dynorphin_A CYLD (human) inhibit treatment-induced increase Downstream Regulation Effect of modification (function):  protein degradation, receptor internalization, induced Comments:  ubiquitination regulates but is not required for receptor downregulation