Dammer EB, Sewer MB (2008) Phosphorylation of CtBP1 by cAMP-dependent protein kinase modulates induction of CYP17 by stimulating partnering of CtBP1 and 2. J Biol Chem 283, 6925-34 18184656

This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.

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S100-p - CTBP1 (human) ▲

Modsite: RIIVRIGsGFDNIDI SwissProt Entrez-Gene Orthologous residues CTBP1 (human): S100‑p, CTBP1 iso2 (human): S89‑p, CTBP1 (mouse): S100‑p, CTBP1 (rat): S89‑p Characterization Methods used to characterize site in vivo:  [32P] ATP in vitro, immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  NCI-H295R Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE PAK6 (human) Downstream Regulation Effect of modification (function):  molecular association, regulation Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays SF1 (human) Induces mammalian two-hybrid

T144-p - CTBP1 (human) ▲

Modsite: LNLYRRAtWLHQALR SwissProt Entrez-Gene Orthologous residues CTBP1 (human): T144‑p, CTBP1 iso2 (human): T133‑p, CTBP1 (mouse): T144‑p, CTBP1 (rat): T133‑p Characterization Methods used to characterize site in vivo:  [32P] ATP in vitro, immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  NCI-H295R Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE PKACA (human) Downstream Regulation Effect of modification (function):  molecular association, regulation, protein conformation Effect of modification (process):  transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays CTBP2 (human) Induces co-immunoprecipitation GCN5 (human) Disrupts mammalian two-hybrid

S158-p - CTBP1 (human) ▲

Modsite: REGTRVQsVEQIREV SwissProt Entrez-Gene Orthologous residues CTBP1 (human): S158‑p, CTBP1 iso2 (human): S147‑p, CTBP1 (mouse): S158‑p, CTBP1 (rat): S147‑p Characterization Methods used to characterize site in vivo:  [32P] ATP in vitro, immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  NCI-H295R Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE PAK6 (human) Downstream Regulation Effect of modification (function):  molecular association, regulation Effect of modification (process):  transcription, induced Modification regulates interactions with:  Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays STF-1 (human) Disrupts co-immunoprecipitation

S164-p - CTBP2 (human) ▲

Modsite: REGTRVQsVEQIREV SwissProt Entrez-Gene Orthologous residues CTBP2 (human): S164‑p, CTBP2 iso2 (human): S704‑p, CTBP2 (mouse): S164‑p, CTBP2 iso2 (mouse): S707‑p, CTBP2 (rat): S164‑p, CTBP2 iso2 (rat): S707‑p Characterization Methods used to characterize site in vivo:  [32P] ATP in vitro, immunoprecipitation, mutation of modification site, western blotting Relevant cell lines - cell types - tissues:  NCI-H295R Cellular systems studied:  cell lines Species studied:  human Enzymes shown to modify site in vitro:  Type Enzyme KINASE PAK6 (human) KINASE PKACA (human)