Curated Information
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Home > Curated Information Page > PubMed Id: 18032450
Takatori A, et al. (2008) Differential transmission of MEKK1 morphogenetic signals by JNK1 and JNK2. Development 135, 23-32 18032450
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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T203-p - ERK1 (mouse)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p

Y205-p - ERK1 (mouse)
Modsite: HtGFLtEyVAtRWyR SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p

T183-p - ERK2 (mouse)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p

Y185-p - ERK2 (mouse)
Modsite: HtGFLtEyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p

T183-p - JNK1 (mouse)
Modsite: AGtsFMMtPyVVtRY SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): T183‑p, JNK1 iso2 (human): T183‑p, JNK1 iso3 (human): T183‑p, JNK1 (mouse): T183‑p, JNK1 (rat): T183‑p
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), ES (stem)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE MKK4 (mouse)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MKK4 (mouse) genetic knockout/knockin of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TGF-alpha increase JNK1 phosphorylation was highly induced, JNK2 was much weaker
TGF-beta increase JNK1 phosphorylation was highly induced, JNK2 was much weaker
activin MEK1 (mouse) increase JNK1 phosphorylation was highly induced, JNK2 was much weaker, MEKK1 is required
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Effect of modification (process):  cell motility, induced, transcription, inhibited
Comments:  GTS sequence motif within the activation loop offers a higher phosphorylation efficiency and therefore functional activation ofJNK1 over JNK2., transcription leading to gene expression, epithelial cell migration and eyelid closure.

Y185-p - JNK1 (mouse)
Modsite: tsFMMtPyVVtRYYR SwissProt Entrez-Gene
Orthologous residues
JNK1 (human): Y185‑p, JNK1 iso2 (human): Y185‑p, JNK1 iso3 (human): Y185‑p, JNK1 (mouse): Y185‑p, JNK1 (rat): Y185‑p
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), ES (stem)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE MKK4 (mouse)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MKK4 (mouse) genetic knockout/knockin of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TGF-alpha increase JNK1 phosphorylation was highly induced, JNK2 was much weaker
TGF-beta increase JNK1 phosphorylation was highly induced, JNK2 was much weaker
activin MEK1 (mouse) increase JNK1 phosphorylation was highly induced, JNK2 was much weaker, MEKK1 is required
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Effect of modification (process):  cell motility, induced, transcription, inhibited
Comments:  GTS sequence motif within the activation loop offers a higher phosphorylation efficiency and therefore functional activation ofJNK1 over JNK2., transcription leading to gene expression, epithelial cell migration and eyelid closure.

T183-p - JNK2 (mouse)
Modsite: ACTNFMMtPyVVtRY SwissProt Entrez-Gene
Orthologous residues
JNK2 (human): T183‑p, JNK2 iso2 (human): T183‑p, JNK2 iso3 (human): T183‑p, JNK2 (mouse): T183‑p, JNK2 (rat): T183‑p
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), ES (stem)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE MKK4 (mouse)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MKK4 (mouse) genetic knockout/knockin of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TGF-alpha increase JNK1 phosphorylation was highly induced, JNK2 was much weaker
TGF-beta increase JNK1 phosphorylation was highly induced, JNK2 was much weaker
activin MEK1 (mouse) increase JNK1 phosphorylation was highly induced, JNK2 was much weaker, MEKK1 is required
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Effect of modification (process):  cell motility, induced, transcription, inhibited
Comments:  GTS sequence motif within the activation loop offers a higher phosphorylation efficiency and therefore functional activation ofJNK1 over JNK2., transcription leading to gene expression, epithelial cell migration and eyelid closure.

Y185-p - JNK2 (mouse)
Modsite: TNFMMtPyVVtRYYR SwissProt Entrez-Gene
Orthologous residues
JNK2 (human): Y185‑p, JNK2 iso2 (human): Y185‑p, JNK2 iso3 (human): Y185‑p, JNK2 (mouse): Y185‑p, JNK2 (rat): Y185‑p
Characterization
Methods used to characterize site in vivo immunoassay, immunoprecipitation, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  293 (epithelial), ES (stem)
Cellular systems studied:  cell lines
Species studied:  human, mouse
Enzymes shown to modify site in vitro
Type Enzyme
KINASE MKK4 (mouse)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE MKK4 (mouse) genetic knockout/knockin of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TGF-alpha increase JNK1 phosphorylation was highly induced, JNK2 was much weaker
TGF-beta increase JNK1 phosphorylation was highly induced, JNK2 was much weaker
activin MEK1 (mouse) increase JNK1 phosphorylation was highly induced, JNK2 was much weaker, MEKK1 is required
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced
Effect of modification (process):  cell motility, induced, transcription, inhibited
Comments:  GTS sequence motif within the activation loop offers a higher phosphorylation efficiency and therefore functional activation ofJNK1 over JNK2., transcription leading to gene expression, epithelial cell migration and eyelid closure.

S63-p - Jun (mouse)
Modsite: kNsDLLtsPDVGLLK SwissProt Entrez-Gene
Orthologous residues
Jun (human): S63‑p, Jun (mouse): S63‑p, Jun (rat): S63‑p, Jun (cow): S63‑p

S255-p - MKK4 (mouse)
Modsite: ISGQLVDsIAKtRDA SwissProt Entrez-Gene
Orthologous residues
MKK4 (human): S257‑p, MKK4 iso2 (human): S268‑p, MKK4 (mouse): S255‑p, MKK4 (rat): S255‑p

T259-p - MKK4 (mouse)
Modsite: LVDsIAKtRDAGCRP SwissProt Entrez-Gene
Orthologous residues
MKK4 (human): T261‑p, MKK4 iso2 (human): T272‑p, MKK4 (mouse): T259‑p, MKK4 (rat): T259‑p

S287-p - MKK7 (mouse)
Modsite: ISGRLVDsKAKtRSA SwissProt Entrez-Gene
Orthologous residues
MKK7 (human): S271‑p, MKK7 iso3 (human): S287‑p, MKK7 (mouse): S287‑p, MKK7 iso2 (mouse): S271‑p, MKK7 iso6 (mouse): S287‑p

T291-p - MKK7 (mouse)
Modsite: LVDsKAKtRSAGCAA SwissProt Entrez-Gene
Orthologous residues
MKK7 (human): T275‑p, MKK7 iso3 (human): T291‑p, MKK7 (mouse): T291‑p, MKK7 iso2 (mouse): T275‑p, MKK7 iso6 (mouse): T291‑p

S465-p - SMAD2 (mouse)
Modsite: sPSVRCSsMs_____ SwissProt Entrez-Gene
Orthologous residues
SMAD2 (human): S465‑p, SMAD2 (mouse): S465‑p, SMAD2 (rat): S465‑p, SMAD2 (cow): S465‑p

S467-p - SMAD2 (mouse)
Modsite: SVRCSsMs_______ SwissProt Entrez-Gene
Orthologous residues
SMAD2 (human): S467‑p, SMAD2 (mouse): S467‑p, SMAD2 (rat): S467‑p, SMAD2 (cow): S467‑p