Curated Information
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Home > Curated Information Page > PubMed Id: 12042314
Fujita N, Sato S, Katayama K, Tsuruo T (2002) Akt-dependent phosphorylation of p27Kip1 promotes binding to 14-3-3 and cytoplasmic localization. J Biol Chem 277, 28706-13 12042314
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S58-p - 14-3-3 zeta (human)
Modsite: VVGARRssWRVVssI SwissProt Entrez-Gene
Orthologous residues
14‑3‑3 zeta (human): S58‑p, 14‑3‑3 zeta (mouse): S58‑p, 14‑3‑3 zeta (rat): S58‑p, 14‑3‑3 zeta (sheep): S58‑p

S10-p - p27Kip1 (human)
Modsite: NVRVSNGsPsLErMD SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): S10‑p, p27Kip1 (mouse): S10‑p, p27Kip1 (rat): S10‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  HEK293T (epithelial) [Akt1 (human)], HEK293T (epithelial) [p27Kip1 (human)]
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)

T187-p - p27Kip1 (human)
Modsite: NAGsVEQtPKKPGLR SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): T187‑p, p27Kip1 (mouse): T187‑p, p27Kip1 (rat): T187‑p

T198-p - p27Kip1 (human)
Modsite: PGLRRRQt_______ SwissProt Entrez-Gene
Orthologous residues
p27Kip1 (human): T198‑p, p27Kip1 (mouse): T197‑p, p27Kip1 (rat): T197‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody
Relevant cell lines - cell types - tissues:  HEK293T (epithelial) [Akt1 (human)], HEK293T (epithelial) [p27Kip1 (human)]
Cellular systems studied:  cell lines
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE Akt1 (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE Akt1 (human)
Downstream Regulation
Effect of modification (function):  intracellular localization, molecular association, regulation
Effect of modification (process):  cell cycle regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
14-3-3 theta (human) 14-3-3 Induces intracellular localization co-immunoprecipitation
14-3-3 epsilon (human) 14-3-3 Induces intracellular localization co-immunoprecipitation
14-3-3 eta (human) 14-3-3 Induces intracellular localization co-immunoprecipitation
Comments:  14-3-3-theta binds strongly to phospho-T198 in the co-precipitation assay, while -epsilon and -eta bind weakly.