Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus®
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 20708687
Karyo R, et al. (2010) Identification of eukaryotic elongation factor-2 as a novel cellular target of lithium and glycogen synthase kinase-3. Mol Cell Neurosci 45, 449-55 20708687
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

T57-p - EEF2 (human)
Modsite: rAGEtRFtDtRkDEQ SwissProt Entrez-Gene
Orthologous residues
EEF2 (human): T57‑p, EEF2 (mouse): T57‑p, EEF2 (rat): T57‑p, EEF2 (rabbit): T56‑p, EEF2 (cow): T57‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  SH-SY5Y (neural crest)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
lithium decrease
serum_withdrawal lithium augment treatment-induced decrease low serum
glucose_starvation lithium augment treatment-induced decrease glucose-depleted medium
valproic_acid no change compared to control
carbamazepine no change compared to control
IP(3) no change compared to control
lithium IP(3) decrease
peptide inhibitor increase L803-mts, [N-Myristol-GKEAPPAPPQS(p)P]
TDZD-8 increase
BIO increase BIO-6
SB216763 increase
GSK3B (human) decrease
GSK3A (human) decrease
lithium SB216763 inhibit treatment-induced increase
lithium TDZD-8 inhibit treatment-induced increase

S366-p - EEF2K (human)
Modsite: sPQVRtLsGSRPPLL SwissProt Entrez-Gene
Orthologous residues
EEF2K (human): S366‑p, EEF2K (mouse): S365‑p, EEF2K (rat): S365‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  SH-SY5Y (neural crest)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
glucose increase high glucose
low_glucose decrease

S21-p - GSK3A (human)
Modsite: sGrARtssFAEPGGG SwissProt Entrez-Gene
Orthologous residues
GSK3A (human): S21‑p, GSK3A (mouse): S21‑p, GSK3A (rat): S21‑p, GSK3A (cow): S21‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  SH-SY5Y (neural crest)
Cellular systems studied:  cell lines
Species studied:  human

S9-p - GSK3B (human)
Modsite: SGRPRttsFAEsCkP SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S9‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  SH-SY5Y (neural crest)
Cellular systems studied:  cell lines
Species studied:  human

S240-p - S6 (human)
Modsite: RLssLRAstsKsEss SwissProt Entrez-Gene
Orthologous residues
S6 (human): S240‑p, S6 (mouse): S240‑p, S6 (rat): S240‑p, S6 (fruit fly): S239‑p, S6 (cow): S240‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  SH-SY5Y (neural crest)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SB216763 decrease
lithium increase
GSK3A (human) increase
GSK3B (human) increase

S244-p - S6 (human)
Modsite: LRAstsKsEssQK__ SwissProt Entrez-Gene
Orthologous residues
S6 (human): S244‑p, S6 (mouse): S244‑p, S6 (rat): S244‑p, S6 (fruit fly): V243‑p, S6 (cow): S244‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Disease tissue studied:  neuroblastoma
Relevant cell lines - cell types - tissues:  SH-SY5Y (neural crest)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
SB216763 decrease
lithium increase
GSK3A (human) increase
GSK3B (human) increase

T57-p - EEF2 (mouse)
Modsite: RAGEtRFtDtRKDEQ SwissProt Entrez-Gene
Orthologous residues
EEF2 (human): T57‑p, EEF2 (mouse): T57‑p, EEF2 (rat): T57‑p, EEF2 (rabbit): T56‑p, EEF2 (cow): T57‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, hippocampus'
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
lithium decrease

S9-p - GSK3B (mouse)
Modsite: SGRPRttsFAEsCKP SwissProt Entrez-Gene
Orthologous residues
GSK3B (human): S9‑p, GSK3B iso2 (human): S9‑p, GSK3B (mouse): S9‑p, GSK3B (rat): S9‑p, GSK3B (rabbit): S9‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, hippocampus'
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
lithium increase

S240-p - S6 (mouse)
Modsite: RLssLRAstsKsEss SwissProt Entrez-Gene
Orthologous residues
S6 (human): S240‑p, S6 (mouse): S240‑p, S6 (rat): S240‑p, S6 (fruit fly): S239‑p, S6 (cow): S240‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, hippocampus'
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
lithium increase

S244-p - S6 (mouse)
Modsite: LRAstsKsEssQK__ SwissProt Entrez-Gene
Orthologous residues
S6 (human): S244‑p, S6 (mouse): S244‑p, S6 (rat): S244‑p, S6 (fruit fly): V243‑p, S6 (cow): S244‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  'brain, hippocampus'
Cellular systems studied:  tissue
Species studied:  mouse
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
lithium increase