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Home > Curated Information Page > PubMed Id: 21188622
Waiwut P, et al. (2011) Gomisin N enhances TNF-α-induced apoptosis via inhibition of the NF-κB and EGFR survival pathways. Mol Cell Biochem 350, 169-75 21188622
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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T693-p - EGFR (human)
Modsite: RELVEPLtPsGEAPN SwissProt Entrez-Gene
Orthologous residues
EGFR (human): T693‑p, EGFR iso2 (human): , EGFR iso5 (human): T693‑p, EGFR (mouse): T695‑p, EGFR (rat): T694‑p, EGFR (pig): T693‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
gomisin_A TNF no effect upon treatment-induced increase
gomisin_N TNF no effect upon treatment-induced increase

S1070-p - EGFR (human)
Modsite: DsFLQRyssDPtGAL SwissProt Entrez-Gene
Orthologous residues
EGFR (human): S1070‑p, EGFR iso2 (human): , EGFR iso5 (human): S1070‑p, EGFR (mouse): S1070‑p, EGFR (rat): S1069‑p, EGFR (pig): S1069‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
gomisin_A TNF no effect upon treatment-induced increase
gomisin_N TNF inhibit treatment-induced increase

S1071-p - EGFR (human)
Modsite: sFLQRyssDPtGALt SwissProt Entrez-Gene
Orthologous residues
EGFR (human): S1071‑p, EGFR iso2 (human): , EGFR iso5 (human): S1071‑p, EGFR (mouse): S1071‑p, EGFR (rat): S1070‑p, EGFR (pig): S1070‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
gomisin_A TNF no effect upon treatment-induced increase
gomisin_N TNF inhibit treatment-induced increase

T202-p - ERK1 (human)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): T202‑p, ERK1 iso2 (human): T202‑p, ERK1 iso3 (human): T202‑p, ERK1 (mouse): T203‑p, ERK1 (rat): T203‑p, ERK1 (hamster): T192‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
gomisin_A TNF no effect upon treatment-induced increase
gomisin_N TNF inhibit treatment-induced increase
TNF increase
gomisin_A TNF no effect upon treatment-induced increase
gomisin_N TNF no effect upon treatment-induced increase

Y204-p - ERK1 (human)
Modsite: HtGFLtEyVAtRWyr SwissProt Entrez-Gene
Orthologous residues
ERK1 (human): Y204‑p, ERK1 iso2 (human): Y204‑p, ERK1 iso3 (human): Y204‑p, ERK1 (mouse): Y205‑p, ERK1 (rat): Y205‑p, ERK1 (hamster): Y194‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
gomisin_A TNF no effect upon treatment-induced increase
gomisin_N TNF no effect upon treatment-induced increase

S32-p - IkB-alpha (human)
Modsite: LLDDRHDsGLDsMkD SwissProt Entrez-Gene
Orthologous residues
IkB‑alpha (human): S32‑p, IkB‑alpha (mouse): S32‑p, IkB‑alpha (rat): S32‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
gomisin_A TNF no effect upon treatment-induced increase
gomisin_N TNF inhibit treatment-induced increase

S36-p - IkB-alpha (human)
Modsite: RHDsGLDsMkDEEyE SwissProt Entrez-Gene
Orthologous residues
IkB‑alpha (human): S36‑p, IkB‑alpha (mouse): S36‑p, IkB‑alpha (rat): S36‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
gomisin_A TNF no effect upon treatment-induced increase
gomisin_N TNF inhibit treatment-induced increase

S176-p - IKKA (human)
Modsite: AKDVDQGsLCtsFVG SwissProt Entrez-Gene
Orthologous residues
IKKA (human): S176‑p, IKKA (mouse): S176‑p, IKKA (rat): S176‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
gomisin_A TNF no effect upon treatment-induced increase
gomisin_N TNF inhibit treatment-induced increase

S180-p - IKKA (human)
Modsite: DQGsLCtsFVGTLQY SwissProt Entrez-Gene
Orthologous residues
IKKA (human): S180‑p, IKKA (mouse): S180‑p, IKKA (rat): S180‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
gomisin_A TNF no effect upon treatment-induced increase
gomisin_N TNF inhibit treatment-induced increase

T334-p - MAPKAPK2 (human)
Modsite: QstKVPQtPLHtsRV SwissProt Entrez-Gene
Orthologous residues
MAPKAPK2 (human): T334‑p, MAPKAPK2 iso2 (human): T334‑p, MAPKAPK2 (mouse): T320‑p, MAPKAPK2 (rat): T320‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
gomisin_N TNF inhibit treatment-induced increase
SB203580 TNF inhibit treatment-induced increase
TAK1_inhibitor TNF inhibit treatment-induced increase

S218-p - MKK3 (human)
Modsite: IsGyLVDsVAKtMDA SwissProt Entrez-Gene
Orthologous residues
MKK3 (human): S218‑p, MKK3 iso2 (human): S189‑p, MKK3 iso3 (human): S223‑p, MKK3 (mouse): S218‑p, MKK3 (rat): S290‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
SB203580 TNF augment treatment-induced increase
TAK1_inhibitor TNF inhibit treatment-induced increase

S207-p - MKK6 (human)
Modsite: IsGyLVDsVAKtIDA SwissProt Entrez-Gene
Orthologous residues
MKK6 (human): S207‑p, MKK6 iso2 (human): S151‑p, MKK6 (mouse): S207‑p, MKK6 (rat): S207‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
SB203580 TNF augment treatment-induced increase
TAK1_inhibitor TNF inhibit treatment-induced increase

S536-p - NFkB-p65 (human)
Modsite: sGDEDFSsIADMDFS SwissProt Entrez-Gene
Orthologous residues
NFkB‑p65 (human): S536‑p, NFkB‑p65 (mouse): S534‑p, NFkB‑p65 (rat): S535‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
gomisin_A TNF no effect upon treatment-induced increase
gomisin_N TNF inhibit treatment-induced increase

T180-p - P38A (human)
Modsite: RHtDDEMtGyVAtRW SwissProt Entrez-Gene
Orthologous residues
P38A (human): T180‑p, P38A iso2 (human): T180‑p, P38A (mouse): T180‑p, P38A iso3 (mouse): T180‑p, P38A (rat): T180‑p, P38A (salmonid): T181‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
gomisin_A TNF no effect upon treatment-induced increase
gomisin_N TNF inhibit treatment-induced increase
TNF increase
SB203580 TNF no effect upon treatment-induced increase
TAK1_inhibitor TNF inhibit treatment-induced increase

Y182-p - P38A (human)
Modsite: tDDEMtGyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
P38A (human): Y182‑p, P38A iso2 (human): Y182‑p, P38A (mouse): Y182‑p, P38A iso3 (mouse): Y182‑p, P38A (rat): Y182‑p, P38A (salmonid): Y183‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
gomisin_A TNF no effect upon treatment-induced increase
gomisin_N TNF inhibit treatment-induced increase
TNF increase
SB203580 TNF no effect upon treatment-induced increase
TAK1_inhibitor TNF inhibit treatment-induced increase

T187-p - TAK1 (human)
Modsite: CDIQtHMtNNKGsAA SwissProt Entrez-Gene
Orthologous residues
TAK1 (human): T187‑p, TAK1 (mouse): T187‑p, TAK1 iso3 (mouse): T187‑p, TAK1 (rat): T187‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
gomisin_A TNF no effect upon treatment-induced increase
gomisin_N TNF no effect upon treatment-induced increase
TNF increase
SB203580 TNF augment treatment-induced increase
TAK1_inhibitor TNF inhibit treatment-induced increase

T183-p - ERK2 (rat)
Modsite: HDHtGFLtEyVAtRW SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): T185‑p, ERK2 (mouse): T183‑p, ERK2 (rat): T183‑p, ERK2 (chicken): T193‑p, ERK2 (cow): T185‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
gomisin_A TNF no effect upon treatment-induced increase
gomisin_N TNF no effect upon treatment-induced increase

Y185-p - ERK2 (rat)
Modsite: HtGFLtEyVAtRWYR SwissProt Entrez-Gene
Orthologous residues
ERK2 (human): Y187‑p, ERK2 (mouse): Y185‑p, ERK2 (rat): Y185‑p, ERK2 (chicken): Y195‑p, ERK2 (cow): Y187‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  HeLa (cervical)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
TNF increase
gomisin_A TNF no effect upon treatment-induced increase
gomisin_N TNF no effect upon treatment-induced increase