Curated Information
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Home > Curated Information Page > PubMed Id: 20932475
Hinz M, et al. (2010) A cytoplasmic ATM-TRAF6-cIAP1 module links nuclear DNA damage signaling to ubiquitin-mediated NF-κB activation. Mol Cell 40, 63-74 20932475
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S1981-p - ATM (human)
Modsite: sLAFEEGsQSTtIss SwissProt Entrez-Gene
Orthologous residues
ATM (human): S1981‑p, ATM (mouse): S1987‑p, ATM (rat): S1988‑p
Characterization
Methods used to characterize site in vivo peptide sequencing, western blotting
Disease tissue studied:  liver cancer
Relevant cell lines - cell types - tissues:  HepG2 (hepatic)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase membrane, cytosol, and nuclear extracts
KU-55933 ionizing_radiation inhibit treatment-induced increase
ionizing_radiation IKKG (human) no effect upon treatment-induced increase

S32-p - IkB-alpha (human)
Modsite: LLDDRHDsGLDsMkD SwissProt Entrez-Gene
Orthologous residues
IkB‑alpha (human): S32‑p, IkB‑alpha (mouse): S32‑p, IkB‑alpha (rat): S32‑p
Characterization
Methods used to characterize site in vivo peptide sequencing, western blotting
Disease tissue studied:  liver cancer
Relevant cell lines - cell types - tissues:  HepG2 (hepatic)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
ionizing_radiation IKKG (human) inhibit treatment-induced increase K285R IKKG mutant

S36-p - IkB-alpha (human)
Modsite: RHDsGLDsMkDEEyE SwissProt Entrez-Gene
Orthologous residues
IkB‑alpha (human): S36‑p, IkB‑alpha (mouse): S36‑p, IkB‑alpha (rat): S36‑p
Characterization
Methods used to characterize site in vivo peptide sequencing, western blotting
Disease tissue studied:  liver cancer
Relevant cell lines - cell types - tissues:  HepG2 (hepatic)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
ionizing_radiation IKKG (human) inhibit treatment-induced increase K285R IKKG mutant

S176-p - IKKA (human)
Modsite: AKDVDQGsLCtsFVG SwissProt Entrez-Gene
Orthologous residues
IKKA (human): S176‑p, IKKA (mouse): S176‑p, IKKA (rat): S176‑p
Characterization
Methods used to characterize site in vivo peptide sequencing, western blotting
Disease tissue studied:  liver cancer
Relevant cell lines - cell types - tissues:  HepG2 (hepatic)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
ionizing_radiation CLASP1 (human) increase siRNA CLASP1 inhibits
ionizing_radiation IKKG (human) inhibit treatment-induced increase K285R IKKG mutation

S180-p - IKKA (human)
Modsite: DQGsLCtsFVGTLQY SwissProt Entrez-Gene
Orthologous residues
IKKA (human): S180‑p, IKKA (mouse): S180‑p, IKKA (rat): S180‑p
Characterization
Methods used to characterize site in vivo peptide sequencing, western blotting
Disease tissue studied:  liver cancer
Relevant cell lines - cell types - tissues:  HepG2 (hepatic)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
ionizing_radiation CLASP1 (human) increase siRNA CLASP1 inhibits
ionizing_radiation IKKG (human) inhibit treatment-induced increase K285R IKKG mutation

K285-ub - IKKG (human)
Modsite: QEVIDkLkEEAEQHk SwissProt Entrez-Gene
Orthologous residues
IKKG (human): K285‑ub, IKKG iso2 (human): K353‑ub, IKKG (mouse): K278‑ub, IKKG (rat): K278‑ub
Characterization
Methods used to characterize site in vivo mass spectrometry, mutation of modification site
Relevant cell lines - cell types - tissues:  293 (epithelial)
Cellular systems studied:  cell lines
Species studied:  human
Downstream Regulation
Effect of modification (function):  activity, induced
Comments:  activation by genotoxic stress

T184-p - TAK1 (human)
Modsite: GtACDIQtHMtNNKG SwissProt Entrez-Gene
Orthologous residues
TAK1 (human): T184‑p, TAK1 (mouse): T184‑p, TAK1 iso3 (mouse): T184‑p, TAK1 (rat): T184‑p
Characterization
Methods used to characterize site in vivo peptide sequencing, western blotting
Disease tissue studied:  liver cancer
Relevant cell lines - cell types - tissues:  HepG2 (hepatic)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
ionizing_radiation RALBP1 (human) no effect upon treatment-induced increase RALBP1 siRNA
ionizing_radiation TRAF6 (human) no effect upon treatment-induced increase TRAF6 siRNA
BAPTA-AM ionizing_radiation inhibit treatment-induced increase membranes and cytosol fractions
ionizing_radiation ATM (human) increase ATM siRNA inhibits increase
ionizing_radiation UBE2N (human) increase siRNA UBE2N inhibits increase
ionizing_radiation CLASP1 (human) increase CLASP1 siRNA inhibits increase

T187-p - TAK1 (human)
Modsite: CDIQtHMtNNKGsAA SwissProt Entrez-Gene
Orthologous residues
TAK1 (human): T187‑p, TAK1 (mouse): T187‑p, TAK1 iso3 (mouse): T187‑p, TAK1 (rat): T187‑p
Characterization
Methods used to characterize site in vivo peptide sequencing, western blotting
Disease tissue studied:  liver cancer
Relevant cell lines - cell types - tissues:  HepG2 (hepatic)
Cellular systems studied:  cell lines
Species studied:  human
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ionizing_radiation increase
ionizing_radiation RALBP1 (human) no effect upon treatment-induced increase RALBP1 siRNA
ionizing_radiation TRAF6 (human) no effect upon treatment-induced increase TRAF6 siRNA
BAPTA-AM ionizing_radiation inhibit treatment-induced increase membranes and cytosol fractions
ionizing_radiation ATM (human) increase ATM siRNA inhibits increase
ionizing_radiation UBE2N (human) increase siRNA UBE2N inhibits increase
ionizing_radiation CLASP1 (human) increase CLASP1 siRNA inhibits increase