Curated Information
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Home > Curated Information Page > PubMed Id: 21609790
Wu CY, et al. (2011) Identification of the phosphorylation sites in the survival motor neuron protein by protein kinase A. Biochim Biophys Acta 1814, 1134-9 21609790
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
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S4-p - SMN (human)
Modsite: ____MAMssGGsGGG SwissProt Entrez-Gene
Orthologous residues
SMN (human): S4‑p, SMN iso3 (human): S4‑p, SMN (mouse): G4‑p, SMN (rat): G4‑p, SMN (cow):
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro), mutation of modification site
Relevant cell lines - cell types - tissues:  COS7 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKACA (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKACA (human) pharmacological inhibitor of upstream enzyme, transfection of wild-type enzyme, transfection of dominant-negative enzyme, pharmacological activator of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
NKH_477 increase
KT5720 decrease

S5-p - SMN (human)
Modsite: ___MAMssGGsGGGV SwissProt Entrez-Gene
Orthologous residues
SMN (human): S5‑p, SMN iso3 (human): S5‑p, SMN (mouse): S5‑p, SMN (rat): S5‑p, SMN (cow):
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro), mutation of modification site
Relevant cell lines - cell types - tissues:  COS7 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKACA (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKACA (human) pharmacological inhibitor of upstream enzyme, transfection of wild-type enzyme, transfection of dominant-negative enzyme, pharmacological activator of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
NKH_477 increase
KT5720 decrease

S8-p - SMN (human)
Modsite: MAMssGGsGGGVPEQ SwissProt Entrez-Gene
Orthologous residues
SMN (human): S8‑p, SMN iso3 (human): S8‑p, SMN (mouse): A8‑p, SMN (rat): G8‑p, SMN (cow): G4‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro), mutation of modification site
Relevant cell lines - cell types - tissues:  COS7 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKACA (human)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE PKACA (human) pharmacological inhibitor of upstream enzyme, transfection of wild-type enzyme, transfection of dominant-negative enzyme, pharmacological activator of upstream enzyme
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
NKH_477 increase
KT5720 decrease

T85-p - SMN (human)
Modsite: NKsQKkNtAAsLQQW SwissProt Entrez-Gene
Orthologous residues
SMN (human): T85‑p, SMN iso3 (human): T85‑p, SMN (mouse): A82‑p, SMN (rat): A83‑p, SMN (cow): T80‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro), mutation of modification site
Relevant cell lines - cell types - tissues:  COS7 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKACA (human)
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SIP1 (human) Disrupts pull-down assay
GEMIN8 (human) Disrupts pull-down assay

S187-p - SMN (human)
Modsite: sDNIkPksAPWNsFL SwissProt Entrez-Gene
Orthologous residues
SMN (human): S187‑p, SMN iso3 (human): S187‑p, SMN (mouse): A182‑p, SMN (rat): A183‑p, SMN (cow): A182‑p
Characterization
Methods used to characterize site in vivo mass spectrometry (in vitro), mutation of modification site
Relevant cell lines - cell types - tissues:  COS7 (fibroblast)
Cellular systems studied:  cell lines
Species studied:  green monkey
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKACA (human)
Downstream Regulation
Effect of modification (function):  molecular association, regulation
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SIP1 (human) Disrupts pull-down assay
GEMIN8 (human) Disrupts pull-down assay