Curated Information
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Home > Curated Information Page > PubMed Id: 21542582
Gauthier E, Guo X, Mohandas N, An X (2011) Phosphorylation-Dependent Perturbations of the 4.1R-Associated Multiprotein Complex of the Erythrocyte Membrane. Biochemistry 50, 4561-7 21542582
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S312-p - EPB41 iso4 (human)
Modsite: QAQTRQAsALIDRPA SwissProt Entrez-Gene
Orthologous residues
EPB41 (human): S521‑p, EPB41 iso2 (human): S521‑p, EPB41 iso4 (human): S312‑p, EPB41 iso5 (human): S486‑p, EPB41 (mouse): S522‑p, EPB41 iso3 (mouse): , EPB41 iso6 (mouse): S245‑p, EPB41 (rat): S520‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  erythrocyte-blood
Cellular systems studied:  primary cells
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCA (human)
Downstream Regulation
Effect of modification (function):  molecular association, regulation, protein conformation
Effect of modification (process):  cytoskeletal reorganization
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
DARC (human) Induces pull-down assay
GPC1 (human) Induces pull-down assay
SPTA1 (human) Disrupts pull-down assay
XK (human) Induces pull-down assay
Comments:  interactions are induced by phosphorylation of either of the sites

S331-p - EPB41 iso4 (human)
Modsite: RTASKRAsRSLDGAA SwissProt Entrez-Gene
Orthologous residues
EPB41 (human): S540‑p, EPB41 iso2 (human): S540‑p, EPB41 iso4 (human): S331‑p, EPB41 iso5 (human): S505‑p, EPB41 (mouse): S541‑p, EPB41 iso3 (mouse): , EPB41 iso6 (mouse): S264‑p, EPB41 (rat): S539‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  erythrocyte-blood
Cellular systems studied:  primary cells
Species studied:  human
Enzymes shown to modify site in vitro
Type Enzyme
KINASE PKCA (human)
Downstream Regulation
Effect of modification (function):  molecular association, regulation, protein conformation
Effect of modification (process):  cytoskeletal reorganization
Modification regulates interactions with: 
Interacting molecule Interacting domains Effect Consequences (function) Consequences (process) Detection assays
SPTA1 (human) Disrupts pull-down assay
DARC (human) Induces pull-down assay
XK (human) Induces pull-down assay
GPC1 (human) Induces pull-down assay
Comments:  interactions are induced by phosphorylation of either of the sites