Curated Information
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Curated Information Page > PubMed Id: 21561859
Park JH, Arakawa-Takeuchi S, Jinno S, Okayama H (2011) Rho-associated Kinase Connects a Cell Cycle-controlling Anchorage Signal to the Mammalian Target of Rapamycin Pathway. J Biol Chem 286, 23132-41 21561859
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
Information from this record has been curated, but not yet edited in PhosphoSitePlus® and may be incomplete.
Click on the protein name to open the protein page, and on the RSD number to open the site page.
Download

T508-p - LIMK1 (rat)
Modsite: PDRKKRYtVVGNPYW SwissProt Entrez-Gene
Orthologous residues
LIMK1 (human): T508‑p, LIMK1 (mouse): T508‑p, LIMK1 (rat): T508‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  REF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ROCK1 (rat) increase
Y27632 decrease

T389-p - p70S6K iso2 (rat)
Modsite: NQVFLGFtYVAPSVL SwissProt Entrez-Gene
Orthologous residues
p70S6K (human): T412‑p, p70S6K iso2 (human): T389‑p, p70S6K (mouse): T412‑p, p70S6K (rat): T412‑p, p70S6K iso2 (rat): T389‑p, p70S6K (fruit fly): T398‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  REF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
acadesine decrease
staurosporine decrease in WT and TCS2 iso4-Cdc6-Eker cells
Y27632 decrease in WT and TSC2 iso4-Cdc6-Eker cells
cytochalasin_D decrease in WT and TSC iso4-Cdc6-Eker cells
TSC2 iso4 (human) decrease
ROCK1 (rat) decrease

S772-p - Rb (rat)
Modsite: STRPPTLsPIPHIPR SwissProt Entrez-Gene
Orthologous residues
Rb (human): S780‑p, Rb (mouse): S773‑p, Rb (rat): S772‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  REF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ROCK1 (rat) no change compared to control

S799-p - Rb (rat)
Modsite: PGGNIYIsPLKsPYK SwissProt Entrez-Gene
Orthologous residues
Rb (human): S807‑p, Rb (mouse): S800‑p, Rb (rat): S799‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  REF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ROCK1 (rat) decrease

S803-p - Rb (rat)
Modsite: IYIsPLKsPYKISEG SwissProt Entrez-Gene
Orthologous residues
Rb (human): S811‑p, Rb (mouse): S804‑p, Rb (rat): S803‑p
Characterization
Methods used to characterize site in vivo phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  REF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  rat
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
ROCK1 (rat) decrease

T1203-p - TSC2 (rat)
Modsite: EILVRRPtGNTSWLM SwissProt Entrez-Gene
Orthologous residues
TSC2 (human): T1203‑p, TSC2 iso2 (human): T1160‑p, TSC2 iso3 (human): T1159‑p, TSC2 iso4 (human): T1203‑p, TSC2 (mouse): T1203‑p, TSC2 iso6 (mouse): T1203‑p, TSC2 (rat): T1203‑p, TSC2 iso2 (rat): T1160‑p
Characterization
Methods used to characterize site in vivo mutation of modification site, phospho-antibody, western blotting
Relevant cell lines - cell types - tissues:  REF (fibroblast)
Cellular systems studied:  cell lines
Species studied:  rat
Enzymes shown to modify site in vitro
Type Enzyme
KINASE ROCK1 (rat)
Upstream Regulation
Potential in vivo enzymes for site: 
Type Enzyme Evidence Notes
KINASE ROCK1 (human) phospho-motif antibody, transfection of constitutively active enzyme, pharmacological inhibitor of upstream enzyme, modification site within consensus motif