Curated Information
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Home > Curated Information Page > PubMed Id: 16362041
Zhang D, et al. (2006) Identification of MEKK2/3 serine phosphorylation site targeted by the Toll-like receptor and stress pathways. EMBO J 25, 97-107 16362041
This page summarizes selected information from the record referenced above and curated into PhosphoSitePlus®, a comprehensive online resource for the study of protein post-translational modifications (NAR, 2015, 43:D512-20). To learn more about the scope of PhosphoSitePlus®, click here.
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S520-p - MEKK2 (human)
Modsite: LsGtGMKsVtGtPYW SwissProt Entrez-Gene
Orthologous residues
MEKK2 (human): S520‑p, MEKK2 (mouse): S520‑p, MEKK2 (rat): S520‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase
EGF increase
IL-1b increase
TNF increase
peptidoglycan increase
LTA increase
CpG-1668 increase
UV increase
sorbitol increase
anisomycin no change compared to control
nocodazole no change compared to control
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, phosphorylation
Effect of modification (process):  transcription, altered
Comments:  Mutation of S520A inhibits activation and is the key residue. Double mutation of T522A,T524A reduces activation effect. T522A seems to have the least effect of the three single mutations. The triple STT to AAA mutation is akin to kinase dead. Activation leads to Erk5 and JNKK2 phosphorylation/activation.

T522-p - MEKK2 (human)
Modsite: GtGMKsVtGtPYWMS SwissProt Entrez-Gene
Orthologous residues
MEKK2 (human): T522‑p, MEKK2 (mouse): T522‑p, MEKK2 (rat): T522‑p
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, phosphorylation
Effect of modification (process):  transcription, altered
Comments:  Mutation of S520A inhibits activation and is the key residue. Double mutation of T522A,T524A reduces activation effect. T522A seems to have the least effect of the three single mutations. The triple STT to AAA mutation is akin to kinase dead. Activation leads to Erk5 and JNKK2 phosphorylation/activation.

T524-p - MEKK2 (human)
Modsite: GMKsVtGtPYWMSPE SwissProt Entrez-Gene
Orthologous residues
MEKK2 (human): T524‑p, MEKK2 (mouse): T524‑p, MEKK2 (rat): T524‑p
Downstream Regulation
Effect of modification (function):  enzymatic activity, induced, phosphorylation
Effect of modification (process):  transcription, altered
Comments:  Mutation of S520A inhibits activation and is the key residue. Double mutation of T522A,T524A reduces activation effect. T522A seems to have the least effect of the three single mutations. The triple STT to AAA mutation is akin to kinase dead. Activation leads to Erk5 and JNKK2 phosphorylation/activation.

S526-p - MEKK3 (human)
Modsite: MsGTGMRsVtGtPYW SwissProt Entrez-Gene
Orthologous residues
MEKK3 (human): S526‑p, MEKK3 iso2 (human): S557‑p, MEKK3 (mouse): S526‑p, MEKK3 (rat): S526‑p
Upstream Regulation
Treatments, proteins and their effect on site modification: 
Treatments Referenced Treatments Manipulated Protein Referenced Protein Effect Notes
LPS increase

T528-p - MEKK3 (human)
Modsite: GTGMRsVtGtPYWMS SwissProt Entrez-Gene
Orthologous residues
MEKK3 (human): T528‑p, MEKK3 iso2 (human): T559‑p, MEKK3 (mouse): T528‑p, MEKK3 (rat): T528‑p

T530-p - MEKK3 (human)
Modsite: GMRsVtGtPYWMSPE SwissProt Entrez-Gene
Orthologous residues
MEKK3 (human): T530‑p, MEKK3 iso2 (human): T561‑p, MEKK3 (mouse): T530‑p, MEKK3 (rat): T530‑p